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We narrowed to 3 results for: sam ms2 p65 hsf1

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  1. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...incorporating two MS2 RNA aptamers at the tetraloop and stem-loop 2; 3. The MS2-P65-HSF1 plasmid which expresses...CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM is an engineered protein complex for the transcriptional...not been achieved.” That is, until now. Enter the SAM pooled library In a paper published in Nature in ... expresses the activation helper protein. SAM can be combined with a human genome-wide library to activate...Nature paper reports, “Here we have shown that the SAM system is robust, specific, and can facilitate genome-scale...combined with a compact pooled sgRNA library. Our SAM-mediated screens exhibited a high degree of consistency...References & Further Reading: For more information about SAM, including a tool for getting optimized guide sequences...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ...incorporating two MS2 RNA aptamers at the tetraloop and stemloop 2 The MS2-P65-HSF1 activation helper ...Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops ...marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance...references are below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library...please visit the Addgene SAM library page . Targeted gene activation using SAM There are two sets of 3 ...CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery AAV - Cas9 Mouse CRISPR ( ...information. Full references are below. Return to top Cas9 SAM transcriptional activation plasmids and screening...

  3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...inactive Cas9-VP64, a sgRNA with two MS2 RNA aptamers, and the  MS2-P65-HSF1 helper protein (Konermann et al...library substantially identified more hits than the SAM library approach in a comparison study screening ...screening for vemurafenib resistance in A375 cells. The SAM system (CRISPR/Cas9 Synergistic Activation Mediator... in the depletion of essential genes. In the subsampling analysis of the sgRNAs, Brunello even with only...
Showing: 1 - 3 of 3 results