We narrowed to 3 results for: scramble shrna

1. 15 Years of Addgene: The Top 15 Plasmids

   Type

   Blog Post

   ...lentiviral vector that contains a scrambled shRNA. This scrambled shRNA is used as a negative control for...expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini’s lab is...for mammalian RNAi experiments.  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector...transfer plasmid for cloning and expressing new shRNA sequences. For more information, see the Addgene...particles. It’s capable of inducible expression of shRNA in target cells by the addition of tetracycline/...

2. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

   Type

   Blog Post

   ...item of 2005. shRNA - In 2006, we saw an increase in popularity of empty backbones for shRNA expression,...beta-catenin reporter M50 Super 8x TOPFlash, scramble shRNA for use as a negative control, and empty backbones...

3. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ... insert. pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging ...Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B.1 Determine the optimal 21-mer... Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2...which The RNAi Consortium has built a library of shRNAs directed against 15,000 human and 15,000 mouse ...genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific community...lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct...selection. Figure 1 : Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has...