...single experiment wherein Plasmid A (encoding shRNA against human Gene X in backbone Y) was transfected ...variable is the shRNA. Therefore the empty control vector would be Plasmid A sans shRNA, or backbone Y ...every experiment, an investigator needs a standard against which the results can be compared; results from...Experiment: Knockdown the expression of Gene X using shRNA expressed from a plasmid   Figure 1: Expression ... this result one could simply conclude that the shRNA didn’t work, as the expression level of Gene X in...plasmid delivered into the cells successfully? Is the shRNA target correct? Is the purified plasmid DNA viable...constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected with the test ...

... Lentiviral shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression ... shRNA expression Tet-pLKO-puro - Tet-inducible lentiviral shRNA expression ...Retroviral shRNA expression pSico PGK GFP - Conditional shRNA expression through...strength pBV-Luc - Luciferase reporter plasmid with very low basal activity shRNA/RNAi For gene... different genetic element or want to express shRNA. Choose by: Species-specific expression Epitope ...markers Viral expression and packaging Reporters, shRNA expression, transgenics and genome modification...easily identify your protein using an antibody against that epitope. This could allow you to conduct...

...sgRNA (U6) expression, shRNA/shRNA-miR30 constitutive (H1, U6, 7SK) or inducible shRNA-miR30 (CMV-TO) expression...constitutive or inducible, gene knockdown with shRNA or shRNA-miR30, (2) tamoxifen-inducible CreERT2 gene...alternative to the widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids... issues with fluorescent pH sensors, a set of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash...insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation of its substrate, luciferin...containing Cas9, EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with...gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral vector containing...

...Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and prey... to screen the NCC-1 library of approved drugs against the entire kit; a parallel analysis that successfully...expressing GFP, tetracycline response elements, and shRNAs many of which are available at Addgene. Since GMAP...immunoprecipitated from the cells using the Protein A tag. The luciferase activities can then be measured and normalized... assess interactions. Higher relative firefly luciferase activity in the Co-IP compared of a control Co-IP...sgRNA expression vectors (containing ten guides against each of the 8,158 predicted T. gondii protein-coding...

... within bacteria, while allowing for selection against any bacteria not carrying the plasmid. Additionally...out-populate bacteria with plasmids, thus selecting against the propagation of these plasmids through cell ...frequently accomplished through expression of an shRNA targeting the mRNA of the gene of interest. These...plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity...of interest could be inserted upstream of the luciferase gene to determine the level of transcription ...