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Showing: 1 - 3 of 3 results
  1. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...Rodríguez et al., 2011).  Genetics of SPARC Figure 1: SPARC and SPARC2 use the GAL4-UAS system along with...expression of using the SPARC system. The effector is placed downstream of the rest of the SPARC components and...Isaacman-Beck et al., 2019. Applications for SPARC SPARC technology is versatile and can be applied across...Predictive Activity through Recombinase Competition (SPARC), a genetic tool that allows for the labeling of...reliably label any post-mitotic cells in Drosophila. SPARC was developed by Thomas Clandinin’s lab, and incorporates...These sites are target sequences of PhiC31. In the SPARC system, attP sites are located on either side of...integrates an attP site into an attB site. In the SPARC system, PhiC31 irreversibly recombines one of the...
  2. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...Predictive Activity through Recombinase Competition (SPARC) is a fully genetic tool for labeling just a fraction...titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination sites to selectively...labeled), intermediate (12%), and sparse (4%) levels. SPARC is a versatile system that can be used in many cell...interest in the cells you are studying. Read our SPARC blog post for more details. Figure 2: Dense...
Showing: 1 - 3 of 3 results