Overcoming the AAV Size Limitation for CRISPR Delivery
Type
Blog Post
...Zhang lab created an all-in-one SaCas9 and sgRNA construct using the liver-specific serotype AAV8. Since ...control AAV-GFP. Not only did the AAV-SaCas9-sgRNA constructs mediate genome modification, but they did so ... the catalytic domain of RNA deaminase ADAR2. Constructs containing the ADAR2 truncation ADAR2DD(delta984...direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing). Using this ...