...more? Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged...and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral backbone plasmids here! ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here at Addgene...Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene...tailored deactivation rates Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer...

...tools. pLV hUbC-dCas9-MQ1(Q147L)-EGFP is also available for lentiviral transduction. Lysine-specific Demethylase...from Rudolf Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure 2: dCas9-Tet1 ...Core, as well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9-p300 adds ...cytosine demethylation in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation... methylation and activate transcription. A lentiviral vector with the same modifier, Fuw-dCas9-Tet1CD,...) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted cytosine methylation...methylation in mammalian cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced...

...efficiency. Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...Packaging and Envelope plasmids provide the necessary components to make lentiviral particles. Stable, tunable...add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you ...for in vivo viral delivery. Cas9 mRNA and gRNA Plasmids containing gRNA and Cas9 are used in in vitro ...efficiency of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR ...: Traffic Light Reporter System: Can evaluate lentiviral component delivery as well as genome repair by... by non-homologous end joining (NHEJ) or HDR. EGFP validation of sgRNAs: Can evaluate component delivery...

...Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer ... Tat. Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic... Viral Plasmids Lentiviral Plasmids Lentiviral...of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...Looking for lentiviral plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table... empty plasmid with EGFP marker. See plasmid 14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-...

...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity...  Fig. 2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662). See the Applications section on... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ... A novel RNA targeting system Heat-inducible lentiviral Car constructs Anti-GFP [N86/8R] ready-to-use...Colognori et al 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise transcript...of print. PMID: 36690762.   Heat-inducible lentiviral CAR constructs by: Brian O'Neill  A clever system...

...pcDNA3-EGFP - C-terminal GFP for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...expression pLJM1-EGFP - 3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin Mammalian, ... dedicated Zebrafish Plasmids and Resources collection page Return to top Epitope Tag or Fusion Protein...Backbones Choosing Your Perfect Plasmid Backbone Empty backbones are plasmids containing only the elements...blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging ...Collection NLS Nuclear localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls...Hahn's lab at the University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 ... the same number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches ...the Reuben Harris lab has deposited a reporter plasmid that allows for rapid fluorescence-based quantification...deaminases like APOBEC. To achieve this they created a lentiviral vector system that monitors both APOBEC- and ...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...protein to the DNA. They created plasmids for purification of tTALE-eGFP and tTALE-mCherry. In comparison...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Human whole genome...infecting cells with sgRNAiBAR library containing lentiviral vectors the iBARs can be detected by next generation...populations. Figure 3: Micrographs of wild type (top panels) and cells with high intracellular Ca2+ (bottom...digestion of single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., Sci. Rep. 2019...

... Addgene plasmids #61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding...tracrRNA: This plasmid contains three expression cassettes. To target a given site, the plasmid can be digested...PX165; SpCas9 Return to top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both ...references are below. Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR... recombinase-2A-EGFP-KASH 60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described...sgRNA backbone for cloning new targeted plasmids. The plasmid can be digested using SapI, which will reveal...

...Accessory Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...Coronavirus Plasmids & Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top Do you have...particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing Spike and ...expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing firefly luciferase...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...firefly luciferase. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro - Lentiviral vector expressing ...

...using your plasmids. You can submit "Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable strain. For plasmids containing...easy for you to share plasmids. To achieve this goal, we will archive any plasmids you've deposited with...Spreadsheet". Start your plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose... Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered ...

...was to create a one-stop reference guide for plasmids. We've combined our Plasmids 101 blog posts and ...your desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid lovers...with a plasmid that already has the multicistronic element and reporter cloned in. In these plasmids you ...than two genes. Plasmid Name Multicistronic Element Expression Type MSCV-IRES-EGFP IRES Retroviral ...interest. Some of the plasmids listed in the table above (and their related plasmids) are designed to have... an introduction to plasmids, allowing you to spend less time researching plasmid basic features and spend...May 11.) PubMed.   Read more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular biology...

... Plasmid Collections Cre-lox Plasmids Cre-lox Plasmids Additional...hSyn Lentiviral Wang 86794 pCL20c-MSCV-IRES-CRE Cre MSCV Mammalian Roussel 86805 pLV-EGFP-Cre EGFP-Cre ..., removal of EGFP; See also similar plasmids pSico PGK GFP and pSico PGK puro Lentiviral Jacks 11579 pSicoR...Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch plasmid Mammalian Green 22799 Ai9 Rosa26...DsRED and EGFP Cre recombinase reporter Lentiviral Geijsen 65726 pLV-CMV-LoxP-DsRed-LoxP-eGFP Switches ...EF1alpha-EGFPcre Cre-EGFP fusion EF-1 alpha Mammalian Sauer 11955 pBS505 EF1alpha-EGFPcre* Cre-EGFP fusion... pF CAG luc-EGFP-cre puro Luciferase - EGFP- Cre fusion CAG Mammalian Stringer 68448 TOPO rtTA3-2A-Cre...

...you find what you need.   More Plasmid Resources from Addgene: Plasmids 101 blog series Addgene's Molecular...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...need. You will also have to make sure that your plasmid has been incorporated into the host organism, usually... allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently...basic mammalian expression system and go for a lentiviral or a retroviral backbone. This is considered ...open reading frame (ORF) of interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional...details and guidance for choosing your perfect plasmid backbone. Or just ask –  leave a message in the...

... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP - Zebrafish...Jump to Top Cyan Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine...Jump to Top Green Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids iLOV...mEmerald-pBAD - Bacterial Expression EGFP 488 507 34 6 Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP... to Top Yellow Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids EYFP... to Top Orange Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids mKusabira-Orange...

...from three to two. Lentiviral delivery of BioTAP-tagged dCas9 and gRNAs. Using lentiviral delivery of the...more gRNAs Two separate lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP tag, and 2) gRNAs ...CAPTURE 2.0 to be used with primary cells. Find plasmids for CAPTURE 2.0! When compared to the original...that DNA sequences targeted by the gRNAs were the top enriched sequences in the experiment and there was...

...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...Fortunately, a collection of plasmids comprising the multiple lentiviral expression (MuLE) system from...reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral vector containing...secretory routes in various conditions. The plasmids and lentiviral vectors encoding these proteins can also... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61...

... Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...see our CRISPR/Cas Plasmids: Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries ...inducible inactivation. Find the Robinson plasmids or the Royle plasmids and get to work! Robinson et al...sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping...designed the pOSIP (one-step integration plasmid) series of plasmids, vectors that convey both the sequence...protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids from Scot Wolfe's lab. These...mGRASP plasmids. Additionally, the lab recently described the use of another set of mGRASP plasmids in their...

...Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...envelope plasmid Kasparov Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing envelope plasmid Trono 1733 pCI-VSVG...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis...

.... ID Plasmid Description Gene/Insert PI Return to Top Do you have suggestions for other plasmids that ...Collections Luciferase Plasmids Luciferase Plasmid Collection You may also like... Fluorescent Proteins Blog...luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot Plasmids from 2016...luciferase reporter. These plasmids improve over the original STARR-seq plasmids by using the bacterial ORI...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...luciferase expression plasmids; these are noted with the viral service icon . ID Plasmid Luciferase Type Promoter...broad host range plasmid Attila Karsi 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of...