Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
Type
Blog Post
...antibody.
TRIM21, an E3 ubiquitin ligase, is the basis of TRIM-away. In normal physiology, TRIM21 recruits.... To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding that...changes in expression of TRIM21-associated genes, indicating that TRIM21 overexpression does not aberrantly .... Clift et al. found that addition of exogenous TRIM21 protein along with an antibody targeting the protein...that TRIM21 overexpression did not affect cell proliferation. These cells also did not contain changes ...For small cell populations, co-microinjection of TRIM21 protein and the target antibody works well. In ...treatment. In some primary cell lines, endogenous TRIM21 is sufficient for TRIM-Away, and you only need ...