We narrowed to 599 results for: REV;
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TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....journal’s submission rules for review articles Whether submitting a review by invitation or by your own ...discussing the review topic with your peers. Start reading! I started by reading other reviews because, as...Writing a review article is a wonderful way to develop and exercise your scientist skill set. If you ...you dread the thought of writing a review, or if you’re currently stuck trying to write one, hopefully this...field and are the perfect person to be writing the review! Doing so is a great way to develop your ability...information: basically, a scientist’s tool kit. I wrote a review article on histone modifers in B and T cells with...thing I did was google “how to write a scientific review.” The second thing I did was stare in horror at...
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Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m. -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....co-founders of Revolution Bioengineering. Last year we started a company. Revolution Bioengineering ... how feasible they were, conducting literature reviews and having weekly journal clubs outside of work...process, we had an idea that survived the literature review, survived technical feasibility analysis, and was...accomplish the color change until a few months later. RevBio accelerates with help from SynBio Axlr8r We limped... revbio... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m...., etc. Also, a constant in-flow of individuals wherever the cell cultures are kept will increase the risk...detection of mycoplasmas difficult (Ligasová, 2019). Prevention of mycoplasma contamination The best and the ...humans are the major source of contamination, the prevention steps start from you. If you are careful enough...contamination but it’s a very good practice for prevention. Prepare a small quantity of media stock that...that would be used for the experiment, preventing contamination of the rest. Avoid long exposure of the ...only be used to remove mycoplasma and not for preventing mycoplasma growth. Also, prolonged use of a particular...Conclusion To sum up, it is always important to prevent contamination rather than eradicating it later.... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....ori) which allow it to replicate. How can I prevent recombination in my samples? Plasmid recombination...Huang C, Liang Z, Ma X, Wang N, Huo Y-X (2020) Reversed paired-gRNA plasmid cloning strategy for efficient... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog PostPublishedAug. 13, 2014, 3:49 p.m....pyogenes that locks itself together, to produce irreversible protein-peptide interactions. In a study published... peptide tags, which often form only weak and reversible bonds. Howarth’s team has already demonstrated...SpyCatcher system, to be able to grab on to proteins irreversibly in a genetically encoded way. SpyTag is nice... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog PostPublishedNov. 8, 2018, 3:47 p.m....expression vectors. And more broadly, their data revealed that the longer a natural gene sequence is, the... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....early peer-review attempts took place at the beginning of the 18th century. Yet the peer-review was not ...the DNA, were not peer-reviewed (Baldwin, 2015). At its very core, peer-review is a touchstone of the...peer-reviewer will help spot inconsistencies in the work, missing information that would prevent the study... easier for me to go back and finish the review. Peer-review step 2: Detailed assessment After this initial...the peer-reviewer you wish for The last thing that I wanted to mention about the peer-review, is that ... in reviewing the paper of your colleagues. The young scientists that are reading your peer-review comments...perspective of an author submitting a paper, the peer-review seems like another dragon to slay on the way to... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m.... pRSV-Rev - This 3rd generation lentiviral packaging plasmid from the Trono lab contains the rev gene....Didier Trono lab that expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation...pMD2.G, both deposited from the same lab. Find pRSV-Rev. pMDLg/pRRE - This is a 3rd generation lentiviral...contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2.G. Find ...contains all the necessary elements for packaging, reverse transcription, and integration needed for the production... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog PostPublishedJune 27, 2018, 1:16 p.m....plasmid for expressing a novel reverse transcriptase (RT), MarathonRT. Reverse transcriptases are critical...Plasmids Podcast! MarathonRT: A highly processive reverse transcriptase Article contributed by Will Arnold...outperforms other RTs in this regard and is even able to reverse transcribe the extremely long, highly structured...techniques that are dependent on long, error-free, reverse transcription of RNA such as long-read cDNA sequencing...