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  1. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...2022, under CC-BY-NC-ND license.   Find KRaION and Kbp sensor plasmids here!   Torres Cabán CC, et al. Tuning...receptors. Image adapted from Ino et al. 2022, under CC-BY license.   Find MTRIA sensors here!   Ino D, et...vivo. Image reproduced from Liu et al. 2022, under CC-BY-NC license.   Find JEDI-2P plasmids here! Find...localization. Image adapted from Farahani et al. 2022, under CC-BY license.   Find pYtags plasmids here!   Farahani...changes. Image adapted from Koveal et al. 2022, under CC-BY license.   Find LiLac plasmids here!   Koveal ...transfer. Image reproduced from Lu et al. 2022, under CC-BY-NC-ND license.   Find B-gTEMP plasmids here!   ...
  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...assay. Image reused from Anderson et al., 2024 under CC BY license.    Find Anti-V5 [IPI-SV5-Pk1] Antibody...). Image adapted from Kroupova et al., 2024 under CC BY license. This construct can be easily expressed...: 20 µm. Image reused from Cao et al., 2024 under CC-BY license.  These optimized fluorescent proteins... sgRNAs. Image reused from Neo et al., 2024 under CC-BY license.   Find plasmids for CRISPR/Cas9 in...
  3. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...sgRNAs. Image reused from Walton et al. 2024 under a CC-BY-NC-ND license. In this iteration, the size...cells. Image adapted from Yan et al. 2024 under a CC-BY license. Yan, J., et al. (2024) Improving.... Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license.  Integrating hIBA1a promoter...signal. Image reused from Riedmayr et al. 2023 under a CC-BY license.  The authors used REVeRT to functionally...
  4. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    .... Images adapted from Meiring et al., 2022, under CC-BY 4.0 license. A naturally occurring microtubule-severing... Images adapted from Mahlandt et al., 2023, under CC-BY 4.0 license.   Mahlandt et al. used these Opto-RhoGEFs... Image adapted from Aljiboury et al., 2023, under CC-BY 4.0 license.   Building off their earlier work...Images adapted from Tei et al. 2022, bioRxiv, under a CC-BY-NC-ND 4.0 license.   Just make sure you don’t ...
  5. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...inset). Image reused from Ando et al. 2023, under CC-BY license.   The Miyawaki lab generated a series...from Chuapoco, Flytzanis, Goeden, et al. 2023, under CC-BY license.   Find the AAV.CAP-Mac plasmid here...strains and rats. Figure from Chen et al (2023) under CC-BY license.   Find AAV1-X1 plasmid here! Chen...
  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...plants. Adapted from Vu et al., 2024 bioRxiv, under CC-BY-NC-ND 4.0 International license. With this...procedure. Adapted from Smith et al. 2024 under a CC-BY 4.0 license.  The team deposited an array ...cancer). Image adapted from Wu and Wang 2024 under a CC-BY 4.0 license. Find more neuroscience antibodies...
  7. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...Image reused form Doman and Pandey et al., 2023 under CC-BY 4.0 License. Find PE6 plasmids here!  Doman... Image from Strebinger et al., 2023, reused under CC-BY 4.0 License. Importantly, even commercial ...Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0 license. This system provides a unique...
  8. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ....115.178335 Dokshin GA, Ghanta KS, Piscopo KM, Mello CC (2018) Robust Genome Editing with Short Single-Stranded...Gneid H, Edraki A, Watts JK, Sontheimer EJ, Mello CC (2018) 5′ Modifications Improve Potency and Efficacy.... https://doi.org/10.1101/354480 Ghanta KS, Mello CC (2020) Melting dsDNA Donor Molecules Greatly Improves...Ghanta KS, Seth M, Conte D Jr, Shirayama M, Mello CC (2014) A Co-CRISPR Strategy for Efficient Genome ...
  9. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...immunogenicity scores by predictive algorithms. Used under CC BY from Mattei et.al., 2022.   Limitations of... prediction of therapeutic antibodies. Used under CC BY fromGroell et.al, 2018.   3D models are somewhat...
  10. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 International license.  Arimura ...resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license. Protection by the LEAs ...sites. Image reused from Pandey et al. 2024 under CC-BY 4.0 license.   An, M., Raguram, A., Du, S....
  11. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index....mRNA transcript. By Arunreginald at en.wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org/w/index....
  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...labeling system. Image adapted from Lavis, 2016, under CC-BY. Common self-labeling tags Let’s take a look...Image adapted from (Bottanelli et al., 2016) under CC-BY license. Combining orthogonal self-labeling.... Images reused from (Ulrichs et al., 2023) under CC-BY license.   But these tags aren’t limited to...
  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...Image reproduced from Frank Hayward et al. 2024 under CC-BY license. They did more experiments to see ...Image reproduced from Frank Hayward et al. 2024 under CC-BY license.   Video 1: Fluorescence movie of...
  14. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    .... Image adapted from Aggarwal et al. 2025 under a CC-BY-NC-ND 4.0 International license. While the...Furlanis, Dai, Leyva Garcia et al. 2024 bioRxiv, under a CC-BY-NC-ND 4.0 International license. These plasmids...
  15. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...strands. Image from Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion...
  16. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...of accelerating science! Photo courtesy of under a CC license. Many scientists and advocates have argued...
  17. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...McGee et al., 2024, used under the Creative Commons CC BY-NC-ND license.   Once you have the components...
  18. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...emission via FRET.  Image from Beck et al, 2019 under CC BY 4.0.   FRET Chemigenetic Sensors These types...
  19. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    .... Image reused from Kremer and Nemerow 2015 under CC-BY license.   The promise of adenoviral vectors This...
Showing: 1 - 30 of 33 results