..., H., Wang, X., Liu, N., Li, G., Wu, D., Li, Y., Jin, M., Li, H., Wei, Y., Li, T., Yuan, Y., Shi, L., ...Zhao, D., Li, J., Wang, Y., Li, B., Yang, Y., Hou, X., Wang, H., Wei, Z., Liu, X., Li, Y., Li, S., Liu,... Liu, N., Wei, Y., Zhou, Y., Li, Y., Wu, D., Jin, M., Cui, S., Li, H., Li, G., Zhou, J., Yuan, Y., Zhang... H., Wang, X., Liu, Y., Liu, N., Li, Y., Luo, J., Ma, Q., Wu, D., Li, J., Xu, C., & Yang, H. (2023). Programmable... A collaborative team from the David Liu and Dali Li labs reported a similar tool shortly thereafter, ..., Gao, H., Ru, G., Guo, X., Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa, A. A., Qu, C...Guan, Y., Wang, L., Liu, M., Feng, B., Song, G., … Li, D. (2024). Adenine transversion editors enable precise...

...are reached, how do science labs celebrate accomplishments and their team's hard work? Do succesful scientists...How do you celebrate your lab’s achievements? Tom Ellis highlights the importance of acknowledging his teams...-off via a gift, card, and trip to the pub. Dr. Ellis adds “we probably don’t celebrate enough... I’ll.... What does your lab do for fun as a group? The Ellis Lab self-organizes cinema nights, game nights, and...retreat. How do you manage work-life balance? Tom Ellis reveals “I don't. Ask me again in five years and... cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family spend family...! How does your lab celebrate each other's accomplishments? Any tips on making time to relax and/or enjoy...

...may think about sitting amongst a sea of chairs listening to talks all day. But nope, not at the American...everyone - everywhere! From ‘looking at’ to ‘listening to’ microbes But seeing microbes through a microscope...Shapiro from Caltech presented an exciting way of listening to microbes by using non-invasive methods for ...

...postdoc level) and can include the pressure to publish in high-impact journals, and the requirement for...successfully applied and worked as a technical support specialist for 4 years. Read on for insights from my time...personalities and their expectations. If you are willing to listen to a customer’s concerns and are patient enough...Technical Service Representative, Technical Support Specialist, Technical Support Advisor, Technical Correspondent...everyday, it can be fascinating and exciting to listen to the many individual stories and get (sometimes...no signals are obtained. Or someone may heat an ELISA plate during incubation steps in order to enhance... technical support specialist...

...need help putting your list together. Once you have your list of genes, Green Listed can help make the gRNA...Green Listed software can help you do just this, probably in less than a minute (1). Green Listed is a...screen could be used to validate the list of candidate genes. Green Listed, as of now, contains most of the...process quick and easy. Green Listed - a CRISPR Screen Tool Green Listed is a web-based software tool ... freely accessible though http://greenlisted.cmm.ki.se/. Green Listed can be used to design gRNAs for ... model systems. Use Green Listed to extract gRNAs that target your list of selected genes. Start by ...species not currently included in Green Listed.  Provide the list of genes you want to target with your...

...FLIS and Flies  Drosophila, more commonly known as fruit flies, have been an important animal model for...to their small size and inconspicuous coloring.  FLIS plasmid Recently, the Phuels lab deposited a plasmid...experience. The Frequency Light Interval System, or FLIS, incorporates a bioluminescence protein into the...

...world of science publishing, and an ever increasing number of journals and publishers to choose from, ...several considerations. First, it is important to establish the type of audience you want to reach with your...research may stipulate that your article must be published in an open access journal or by using the open...open access option in a conventional journal. Publishing via open access means that your manuscript will...data resulting from their funding should also be published. All these factors will influence your choice ... are writing, it may be useful to consult the checklists that many journals use to verify whether a manuscript...manuscript needs to be revised before it can be published, this can delay publication and manuscripts may...

...indirect ELISA.   Sandwich ELISA Most commercially available ELISA kits are for a sandwich ELISA. This technique...2019.   Competitive ELISA Similar to a sandwich ELISA, the competitive ELISA is also used to detect...sandwich ELISA. While the most common type of competitive ELISA is a modification of the sandwich ELISA as ... 2019.   Indirect ELISA The indirect ELISA is very similar to a direct ELISA, aside from the detection...heavily on antibodies is ELISA, which stands for Enzyme-linked Immunosorbent Assay. ELISA are used to detect...enzymatic reaction. Types of ELISA There are four different types of ELISAs, which differ in the way the...Boguszewska et al., 2019.       Direct ELISA In a direct ELISA, purified proteins or the experimental ...

...and food safety.  Part of the assay’s versatility lies in the user’s ability to modify the protocol to ...bench.   Indirect ELISAs The indirect ELISA process is similar to that of a direct ELISA, but the indirect...cross-reactivity.  Sandwich ELISAs In the most common type of ELISA, the sandwich ELISA, users first coat a multiwell...Scientists value ELISAs for their accuracy, specificity, and sensitivity (a good ELISA can detect picogram... considerations when using the method. ELISA basics All ELISAs follow the same overall process. First ... four types of ELISA are direct, indirect, sandwich and competitive. In a direct ELISA a reporter enzyme... created with BioRender.com  Direct ELISAs A direct ELISA is the quickest and simplest of all varieties...

...researcher at Imperial College London, Dr. Tom Ellis. Dr. Ellis does research in synthetic biology and bioengineering...construct a synthetic yeast chromosome. Listen to learn more about Dr. Ellis, synthetic biology, synthetic chromosomes...becoming an academic researcher. Listen to the interview with Tom Ellis Podcast breakdown Intro: How Tom...and Addgene  Many thanks to our guest, Tom Ellis! Dr. Ellis is a lead researcher at the Centre for Synthetic...Prof Jim Collins and specialising in engineering gene regulation in yeast. Dr. Ellis is now one of the world's...techniques. Chapter 4: Research projects underway in the Ellis lab including yeast and E. coli engineering, microbial... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...Plasmid 1: B. subtilis knock out and knock down libraries. Listen to Our segment on the B. subtilis libraries...you'll learn about new libraries for studying B. subtilis biology, plasmids for enhancing iPSC production...quarterly newsletter or on our hot plasmids webpage. Listen to this Hot Plasmids episode! Hot plasmids covered...fusions modulate iPSC reprogramming efficiency. Listen to the segment on modulating iPSC reprogramming...Plasmid 3: Regulating telomere length with TZAP. Listen to the segment on regulating telomere length with...Hot Plasmid 4: CRISPR tools for use in plants.  Listen to the segment on CRISPR tools for plants. Special... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...Biosensors (Control) Li New viral service pAAV-hSyn-GRAB_ACh3.0 AAV9 Biosensors Li New viral service ...serotype pAAV-hSyn-GRAB_OT1.0 AAV1, AAV9 Biosensors Li New viral service with multiple serotypes pAAV-...

...Meng, J., Li, N., Liu, J., Zhang, H., Xie, L., Ying, W., Li, D., Shi, L., Sun, Y., Li, Y., Li, Y., Sun,.../doi.org/10.1038/s41467-021-25217-y Zhao, D., Li, J., Li, S., Xin, X., Hu, M., Price, M. A., Rosser, S...Luo, S., Liu, L. D., Zhang, S., Zhang, Y., Wo, Y., Li, N., Wu, S., Gui, T., Wang, B., Luo, Y., Cai, Y.,...10.1038/s41587-020-0609-x Yang, C., Dong, X., Ma, Z., Li, B., Bi, C., & Zhang, X. (2022). Pioneer Factor Improves...around the same time. (The papers were actually published in the same journal on the same day.) This team...

...organized chaos of lab research and PhD studies, there lies a hidden gem that has the potential to redefine ... towards inviting serendipity into your research lies in expanding the scope of your exploration. While...bioinformatics or chemistry, even if your primary focus lies within a biological lab. Engaging in such seminars...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with...

...monoallelic diseases In 2013, a team led by Jinsong Li from the Chinese Academy of Sciences found that mice...technology in different labs for different applications.” Li’s team noted that CRISPR/Cas9 has been used to generate...CRISPR to cure genetic disease in the near future,” Li says, though there are some things to sort out first...

...Yiwen Zhang, a Ph.D. candidate in the Yang Lab, Qi Li, a lecturer at Sichuan Normal University, Jieze Zhang...-1 and 1-2 can be done simultaneously. Image from Li et al., 2021.   The updates involved the following... feedback!   Thank you to our guest bloggers! Qi Li obtained her Ph.D. from the Chinese Academy of Sciences...:2506–2514 . https://doi.org/10.1128/aem.04023-14 Li Q, Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021)...Originally published Apr 13, 2021 and last updated Aug 12, 2021. This post was contributed by guest bloggers...

..., H., Bai, S., Li, C., Ding, R., Xue, N., Lei, Z., Chen, Y., Guan, Y., Siwko, S., … Li, D. (2023). Re-... Xie, L., Yu, W., Wang, Y., Li, L., Yin, S., Yang, L., Hu, H., Han, H., Li, Y., Wang, L., Chen, G., Ma...2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of a fusion of both cytidine...Ma, X., Geng, H., Huang, W., Pang, X., Yang, Z., … Li, D. (2020). Dual base editor catalyzes both cytosine...editor was named ABEmax. In 2020, two papers were published describing additional ABEs evolved from ABE7.10...publication highlights In the table below, you'll find a list of publications we've highlighted in this article...

...j.jconrel.2019.02.009 Wang D, Mou H, Li S, Li Y, Hough S, Tran K, Li J, Yin H, Anderson DG, Sontheimer EJ...Originally published Sep 30, 2014 and last updated Dec 10, 2020 by Benoit Giquel.   Adenoviral vectors...Manuel Gonçalves' lab found in a second paper published in Nature Methods the same year that delivering...

...in the model flowering plant Arabidopsis thaliana (Li et al., 2013; Zhang et al., 2018) and the model bryophyte...7:251–257 . https://doi.org/10.1055/s-2005-837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen ....org/10.1105/tpc.113.112235 Morgens DW, Deans RM, Li A, Bassik MC (2016) Systematic comparison of CRISPR...Zhang D, Chen SL, Gong B-Q, Guo Y, Xu L, Zhang X-N, Li J-F (2018) Engineering Artificial MicroRNAs for Multiplex... from both methods did a more comprehensive gene list emerge that accurately reflected the scope of genes...

... reliable cloning technique that was developed by Li, et al., in 2011. This method is ligation independent...Biology 38 (2016): 145-154. PubMed PMID: 27391134. 2. Li, Chaokun, et al. "FastCloning: a highly simplified...

...dLight sensor here! The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar...respectively). Using GRABDA sensors in experiments The Li lab used GRABDA in drosophila, zebrafish, and mouse...

... https://doi.org/10.1089/hgtb.2017.084 Li Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the...check out this review article published in Reviews in Medical Virology (Li et a., 2018).   Figure...

...Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy, Ethan Li, Pranav Vyas, Nibha Akireddy, Chew...of an accessible supply chain for parts that are listed in the bill of material (BOM) of an assembly, the...worldwide. Distributing hardware via globally established makerspace networks and training users via workshops...studying plankton using flow cells, has become an established alternative to high-priced commercial devices...

...recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge SJ. Nat Methods. 2007 Mar;4(3):251-6....method for sequence- and ligation-independent cloning. Li MZ, Elledge SJ. Methods Mol Biol. 2012;852:51-9. ...

... consider FastCloning, first described by Chaokun Li and colleagues. Here you simply PCR-amplify your ...finished constructs at Addgene!). References 1. Li, Chaokun, et al. "FastCloning: a highly simplified...number is a bit smaller, let’s say 4 or less. The list goes on... In this post, we’ve reviewed many of ...

....2014.09.039 Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying...dCas9, also referred to as CRISPRa, was initially published in 2013 (Bikard et al., 2013, Perez-Pinera et ...

...result from excessive nuclease activity in tissues (Li et al., 2018). 4. Selection marker while engineering....e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology...that blocks type II-A CRISPR-Cas systems. A full list of Acr proteins organized by the type of CRISPR-...

...different leaf infiltration methods Co-cultivation (Li et al., 2009): The co-cultivation of Agrobacterium...Humana Press, 2006. 225-229. PubMed PMID: 16739580. Li, Jian-Feng, et al. "The FAST technique: a simplified...precipitate. GUS is usually the first choice for establishing a new transformation protocol as it can easily...might help to get you started. Once you have established a well working protocol in your lab, it can become...

... a reference of highly expressed genes (Sharp and Li, 1987). In 2000 the Ikemura lab created the Codon...683–691. https://doi.org/10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of...

...s13039-018-0368-2  Liu X, Zhang Y, Chen Y, Li M, Zhou F, Li K, Cao H, Ni M, Liu Y, Gu Z, Dickerson KE,...Originally published Dec 7, 2017 and updated Jul 2, 2020. Promoters may be the star of gene regulation...Elements) method. The original CAPTURE method was published in 2017 and addressed many of the drawbacks of...

...critical that the unknown sample’s concentration lies within the dynamic range of the curve. The dynamic...trendline, which may be good enough if your unknown lies within the linear portion of the graph. It does ...resources on the Addgene blog Antibodies 101: ELISAs The Four ELISAs and When to Use Them A Control for All ...viruses; food allergy labs use standard curves with ELISAs to measure allergen concentrations in food; and...BCA assays have linear trendlines while those for ELISAs and other enzymatic assays are sigmoidal. Once ...

...2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted how chromatin substantially... 1–11. https://doi.org/10.1038/s41587-023-01678-y Li, X., Chen, W., Martin, B. K., Calderon, D., Lee, ...efforts. This holds true for any prime editor experimentalist, and especially shines once large-scale prime...

...Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, Fazzio TG,... SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...

...past and present, but the real promise of Addgene lies in our future. As we near our 20th anniversary, ...’d like to take a moment to reflect on our accomplishments from 2022 and share what we’re looking forward...

...adjacent motif (PAM). ABE7.10 can edit target 'A' if it lies in a small genomic window: at protospacer position...Nature plants (2018): 1. PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice and ...

...Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu, Y. “A non-radioactive method ...PMC164612. 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C. “Buoyancy-Activated Cell Sorting Using Targeted...short list of some other common applications and links to relevant papers and webpages are listed below...such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation is the... EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked Immunosorbent Assay) Southern/Northern... Krijgsveld, J., Katsantoni, E., Heck, A., … Strouboulis, J. “Efficient biotinylation and single-step ...

...Lloyd, K., Jardine, J., Coppola, J., Meijers, R., Li, J., & Springer, T. A. (2024). Synthetic integrin...https://doi.org/10.1101/2024.01.26.577394 Jo, M. H., Li, J., Jaumouillé, V., Hao, Y., Coppola, J., Yan, J...recommended for use in flow cytometry. Based on the established applications of the original hybridoma monoclonals...

...in bioluminescent organisms, but their real power lies in the clever ways scientists have adapted them ...

...doi.org/10.1104/pp.15.00636 Ma X, Zhang X, Liu H, Li Z (2020) Highly efficient DNA-free plant genome editing... . https://doi.org/10.1073/pnas.1818290116 Zhu H, Li C, Gao C (2020) Applications of CRISPR–Cas in agriculture...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...

...Zhang X, Su J, Jeong M, Gundry MC, Huang Y-H, Zhou Y, Li W, Goodell MA (2017) Targeted DNA methylation in ...publication at Addgene. Xu X, Tao Y, Gao X, Zhang L, Li X, Zou W, Ruan K, Wang F, Xu G, Hu R (2016) A CRISPR-based...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...are also available as controls. For an up-to-date list of CRISPR epigenetic tools, check out our CRISPR...