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We narrowed to 39 results for: feng zhang

Showing: 1 - 39 of 39 results

  1. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute and was designed ...PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries ...take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR v2 has only been available...

  2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et...producing fluorescent signal. Image modified from Feng et al. 2017 under CC-BY license. Many split-FP...fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017 Bo Huang mNeonGreen3 mNG3K...protein labeling and signal amplification. Zhou S, Feng S, Brown D, Huang B. PLoS One. 2020 Bo Huang Yellow...fluorescent proteins for endogenous protein labeling. Feng et al. Nat Commun. 2017 Bo Huang sfCherry3 Improved...visualization of endogenous proteins and synapses. Feng S et al. Commun Biol. 2019 Bo Huang Near-Infrared...protein-protein interactions in living cells. Shao S, Zhang H, Zeng Y, Li Y, Sun C, Sun Y. Commun Biol. 2021...

  3. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, Y., Dong, A....which helped researchers visualize norepinephrine (Feng et al., 2019). But this first generation of tools...A., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., ...

  4. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...graduate student Silvana Konermann first entered Feng Zhang’s lab at MIT, the use of customizable DNA-binding...new. Now, Konermann and her colleagues including Zhang and Mark Brigham have taken the technology to another...literally at the flip of a (blue) light switch. Zhang’s team devised the plasmids now available in Addgene...introducing the optogenetic tool, Konermann and Zhang showed they could package LITEs in viral vectors...specific epigenetic modifications in the genome,” Zhang said in MIT’s press release. In an accompanying ...

  5. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...example, the authors Patrick Hsu, Eric Lander, and Feng Zhang acknowledge the role of data and materials repositories...

  6. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...with all these questions, Dr. Le Cong and Dr. Feng Zhang developed a Google Forum to allow scientists ...more Q&As which focus on genome editing using the Zhang lab's CRISPR/Cas plasmids. Designing your CRISPR...Double nickase' is a new system, developed by the Zhang lab, which has comparable efficiency to the optimized...nickase system, including one recently from the Zhang group. Learn more here. Q2: When designing oligos...without the G. Because the G is only one base, the Zhang lab usually adds it when they order the oligo. If...

  7. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ... Speaking of CRISPRs, we were excited to have Feng Zhang (of the Broad Institute of MIT and Harvard and...depositing scientist – stop by our booth to say hi! Zhang was awarded one of this year’s SfN Young Investigator...

  8. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...screening - An excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production...using CRISPR/Cas Systems Forum- Moderated by the Feng Zhang lab, this is a useful place to ask experimental...Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional...

  9. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...screening. They and their colleagues including Feng Zhang used the GeCKO library to identify genes essential...titer due to the smaller size of the plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single...Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science. 2013 Dec 12. (PubMed). Genome-Wide Recessive...

  10. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...in December, Konermann and her colleagues in Feng Zhang’s lab describe just such a system, which they ...perfect complement for one conducted earlier by the Zhang lab for GeCKO, a lentiviral CRISPR library enabling...enabling genome-scale, knockout screening). As the Zhang lab's Nature paper reports, “Here we have shown ... Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O. Cell. 2014 Feb 27;156(5):935-49. doi... Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2015 Jan 29;517(7536):583-8. doi: 10.1038...

  11. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...: 237-243. PubMed PMID: 20882. Mei, Yuan, and Feng Zhang. "Molecular tools and approaches for optogenetics... 28772120. PubMed Central PMCID: PMC5830081. Zhang, Feng, et al. "The microbial opsin family of optogenetic...membrane potential in response to visible light (Zhang et al., 2011). What do microbial rhodopsins and ...electrophysiological methods (Deisseroth, 2011; Mei and Zhang, 2012; Towne et al., 2016). Optogenetic neuromodulation...neural activity (Bernstein and Boyden, 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins...

  12. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and multiple ...a smaller, but equally potent Cas9 protein, the Zhang lab took a different approach. They analyzed over...compare the off-target effects of SpCas9 and SaCas9, Zhang’s group used an approach called BLESS (direct in ... test the efficiency of AAV-SaCas9 in vivo, the Zhang lab created an all-in-one SaCas9 and sgRNA construct... Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR... Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13. Science ... Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O (2014) Crystal Structure of Cas9 in ...

  13. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...  Improved nucleic acid sequence detection Feng Zhang’s lab recently developed a platform called SHERLOCK...

  15. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...study author F. Ann Ran, a graduate student in Feng Zhang's lab, in a press release. "In the future, it ...

  16. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...incompatible to genome editing in human cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii...

  17. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...plasmids   Cytosine deaminase for RNA editing Feng Zhang’s lab previously developed a programmable adenosine-to-inosine...

  18. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...s41587-023-02050-w Yi, Z., Zhang, X., Wei, X., Li, J., Ren, J., Zhang, X., Zhang, Y., Tang, H., Chang, X....Hong, M., Luan, C., Gao, H., Ru, G., Guo, X., Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa...., Qu, C., Zhu, Y., Guan, Y., Wang, L., Liu, M., Feng, B., Song, G., … Li, D. (2024). Adenine transversion...., Cui, S., Li, H., Li, G., Zhou, J., Yuan, Y., Zhang, H., Shi, L., Yao, X., & Yang, H. (2023). Programmable... H., Wei, Z., Liu, X., Li, Y., Li, S., Liu, Y., Zhang, X., & Bi, C. (2024). Glycosylase-based base editors...

  19. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas...In addition to publishing in Science, Professor Zhang deposited the associated plasmids with Addgene. ...

  20. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...Note: Cpf1 is also called Cas12a. In 2015, Feng Zhang’s lab characterized two Cpf1 nucleases, distant...regulatory elements. To solve this problem, the Zhang lab has previously used SaCas9 (1053 aa/3.2 kb) ...SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cpf1 Is a Single RNA-Guided Endonuclease of a...

  22. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., ...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...

  23. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9...of a sunny day in Edinburgh.   References 1. Gao, Feng, et al. "DNA-guided genome editing using the Natronobacterium...

  24. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al...end joining (NHEJ). Designing an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13...Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA editing toolkit to allow C ...

  25. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex ...Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease...

  27. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...) Cas13a targeting with a single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a...Ladha et al., 2020). Cas13a in mammalian cells The Zhang lab made the exciting discovery that some Cas13a...

  28. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences to...

  30. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED (...

  31. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries ...different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini (6),...

  32. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...lentiCas9-Blast (Plasmid #52962) was a gift from Feng Zhang and is described in Improved vectors and genome-wide...libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038...

  33. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from....0029875 Pubmed PMID: 22253811 Ni B, Du Z, Guo Z, Zhang Y, Yang R (2008) Curing of four different plasmids...

  35. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...

  37. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...PMC2629495. Zingg, B., Chou, X., Zhang, Z., Mesik, L., Liang, F., Tao, H.W., and Zhang, L.I. (2017). AAV-mediated... S.A., Slaughterbeck, C.R., Wakeman, W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K.E., Bohn,...

  38. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...Cummins, A., Chen, Y., Fan, Y., Zhang, Q., Sharma, J., Duan, Y., Wang, L., Feng, G., Chen, Y., Ip, N. Y., Pickel....2022.05.003 Chen, X., Wolfe, D. A., Bindu, D. S., Zhang, M., Taskin, N., Goertsen, D., Shay, T. F., Sullivan...Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox, G. A., Wade, K., He, J., ...Schneider, G., Stevenson, O., Vergara, J., Sharma, J., Zhang, Q., Franken, T. P., Smith, J., Ibrahim, L. A., ...

  39. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...domain and a mostly c-terminal nuclease domain. Feng Zhang's group utilized the bi-lobed architecture of ... of varying fusion protein expression levels. Zhang et al., PLoS One. 2012;7(8):e43072. doi: 10.1371...
Showing: 1 - 39 of 39 results