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  1. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ... PubMed PMID: 25114216. PubMed Central PMCID: PMC4151723. 2. Doench, John G., et al. "Optimized sgRNA ... PubMed PMID: 26780180. PubMed Central PMCID: PMC4744125. 3. Gilbert, Luke A., et al. "Genome-scale CRISPR-mediated... PubMed PMID: 25307932. PubMed Central PMCID: PMC4253859. 4. Hart, Traver, et al. "High-Resolution CRISPR... PubMed PMID: 25494202. PubMed Central PMCID: PMC4420636. 7. Ma, Hongming, et al. "A CRISPR-based screen... PubMed PMID: 26190106. PubMed Central PMCID: PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9... PubMed PMID: 24336571. PubMed Central PMCID: PMC4089965. 9. Wang, Tim, et al. "Genetic screens in human... PubMed PMID: 26280411. PubMed Central PMCID: PMC4785103. 11. Wong, Alan SL, et al. "Multiplexed barcoded...

  2. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... PubMed PMID: 25337876. PubMed Central PMCID: PMC4270925. Find plasmids from this paper at Addgene. ... PubMed PMID: 25122746. PubMed Central PMCID: PMC4231726. Find plasmids from this paper at Addgene. ... PubMed PMID: 24954249. PubMed Central PMCID: PMC4066266. Find plasmids from this paper at Addgene. ... PubMed PMID: 25751063. PubMed Central PMCID: PMC4396471. Find plasmids from this paper at Addgene. ... PubMed PMID: 24770325. PubMed Central PMCID: PMC4090141. Find plasmids from this paper at Addgene. ... PubMed PMID: 25432517. PubMed Central PMCID: PMC4262988. Find plasmids from this paper at Addgene. ... PubMed PMID: 25733849. PubMed Central PMCID: PMC4371917. Find plasmids from this paper at Addgene. ...

  3. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ... PubMed PMID: 24291815. PubMed Central PMCID: PMC4101921. 19. Dias, J., et al., Generation of red blood... PubMed PMID: 24930130. PubMed Central PMCID: PMC4169698. 24. Lian, X., et al., Robust cardiomyocyte differentiation... PubMed PMID: 26214132. PubMed Central PMCID: PMC4566857. 26. Maffioletti, S.M., et al., Efficient derivation... PubMed PMID: 25578634. PubMed Central PMCID: PMC4412587. 28. Mohsen-Kanson, T., et al., Differentiation... PubMed PMID: 24510784. PubMed Central PMCID: PMC4096605. 30. Lamba, D.A., et al., Generation, purification... PubMed PMID: 27013738. PubMed Central PMCID: PMC4835250. 32. Ma, L., Y. Liu, and S.C. Zhang, Directed... PubMed PMID: 25782665. PubMed Central PMCID: PMC4363833. 35. Eguizabal, C., et al., Dedifferentiation...

  4. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ... PubMed PMID: 24509632. PubMed Central PMCID: PMC4063274. 2. Findlay, S. D., Vincent, K. M., Berman, J... (2016). PubMed PMID: 27089539. PubMed PMCID: PMC4835065. 3. Mock, U., Hauber, I. & Fehse, B. Digital ... PubMed PMID: 25964300. PubMed Central PMCID: PMC4477672. 5. Sedlak, R. H. et al. Digital detection of... PubMed PMID: 26829887. PubMed Central PMCID: PMC4735761. 6. Fu, Y. et al. High-frequency off-target mutagenesis... PubMed PMID: 25566793. PubMed Central PMCID: PMC4349094.  ...

  5. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ... PubMed PMID: 25184501. PubMed Central PMCID: MC4262738. 2. Doench, John G., et al. "Optimized sgRNA design... PubMed PMID: 26780180. PubMed Central PMCID: PMC4744125.  3. Gilbert, Luke A., et al. "Genome-scale CRISPR-mediated... PubMed PMID: 25307932. PubMed Central PMCID: MC4253859.  4. Konermann, Silvana, et al. "Genome-scale .... PubMed PMID: 25494202. PubMed Centra PMCID: MC4420636.  5. Sanson, Kendall R., et al. "Up, down, and... PubMed PMID: 25307933. PubMed Central PMCID: PMC4252608.  Additional Resources on the Addgene Blog Read...

  6. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ... PubMed PMID: 25713381. PubMed Central PMCID: PMC4364232. 2. Baker, Monya. “RNA imaging in situ”. Nature... PubMed PMID: 26000751. PubMed Central PMCID: PMC4441765. 4. Guo, Peixuan AND Haque, Farzin. RNA Nanotechnology... PubMed PMID: 25019067. PubMed Central PMCID: PMC4071757. 6. Hong, He, et al. “Molecular Imaging with ... PubMed PMID: 27446908. PubMed Central PMCID: PMC4923196. 8. Sassolas, Audrey, et al. “Optical detection... PubMed PMID: 26877022. PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based...

  7. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ... PubMed PMID: 25837586. PubMed Central PMCID: PMC4383620. Botman, Dennis, et al. "In vivo characterisation... PubMed PMID: 27240257. PubMed Central PMCID: PMC4927352. Janetopoulos, Chris, Tian Jin, and Peter Devreotes... PubMed PMID: 25875503. PubMed Central PMCID: PMC4397040. Lambert, Talley J. "FPbase: A community-editable... PubMed PMID: 15247428. PubMed Central PMCID: PMC490022. Shaner, Nathan C., et al. "Improving the photostability... PubMed PMID: 26799488. PubMed Central PMCID: PMC4723041. Additional resources on the Addgene blog Read...

  8. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ... PubMed PMID: 25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput... PubMed PMID: 25409780. PubMed Central PMCID: PMC4246457. 4. Davidson CJ., et al. "Improving the limit... PubMed PMID: 25595557. PubMed Central PMCID: PMC4308725. 7. Pattanayak, Vikram, et al. "High-throughput... PubMed PMID: 25513782. PubMed Central PMCID: PMC4320685. 9. Kim, Daesik, et al. "Digenome-seq: genome-wide... PubMed PMID: 25503383. PubMed Central PMCID: PMC4320661. 14. Crosetto, Nicola, et al. "Nucleotide-resolution...

  9. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ... Pubmed PMID: 24733905. Pubmed Central PMCID: PMC4000813. 2. McDowell, Gary S., et al. "Shaping the Future... Pubmed PMID: 25653845. Pubmed Central PMCID: PMC4304227. 3. Sauermann, Henry, and Michael Roach. "Science... Pubmed PMID: 25925677. Pubmed Central PMCID: PMC4484856. 5. Bourne, Henry R. "A fair deal for PhD students... Pubmed PMID: 25291257. Pubmed Central PMCID: PMC4185419. 7. NIH 2012. Biomedical research workforce working...

  10. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...). PubMed: PMID 252409288. PubMed Central: PMCID PMC4˚237163. Find plasmids from this paper at Addgene...PubMed: PMID 25282355. PubMed Central: PMCID: PMC4317352. 3. Beisel, Chase L., Ahmed A. Gomaa, and Rodolphe... PubMed: PMID 25417800. PubMed Central: PMCID PMC4282009. 4. Yosef, Ido, Miriam Manor, Ruth Kiro, and ... PubMed: PMID 26060300. PubMed Central: PMCID PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. ...

  11. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... PubMed PMID: 25555356. PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R.,... PubMed PMID: 24694006. PubMed Central PMCID: PMC4137353. Chan, K.Y., Jang, M.J., Yoo, B.B., Greenbaum... PubMed PMID: 26696844. PubMed Central PMCID: PMC4678200.  Kohara, K., Pignatelli, M., Rivest, A.J., Jung... PubMed PMID: 24336151. PubMed Central PMCID: PMC4004172. Kristensson, K., and Olsson, Y. (1971). Retrograde... PubMed PMID: 25374356. PubMed Central PMCID: PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi...

  12. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ... PubMed PMID: 26611576. PubMed Central PMCID: PMC4971574. Hooper, Andrew, and Jamie Maguire. "Characterization... PubMed PMID: 26934955. PubMed Central PMCID: PMC4987841. Lodish, Harvey et al. Molecular Cell Biology... PubMed PMID: 24700321. PubMed Central PMCID: PMC4100247. Taylor, Norman E., et al. "Optogenetic activation... PubMed PMID: 22196724. PubMed Central PMCID: PMC4166436. Resources on the Addgene blog Learn more about...

  13. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ... PubMed PMID: 25713381. PubMed Central PMCID: PMC4364232. Find the plasmids from this publication at ... PubMed PMID: 27088723. PubMed Central PMCID: PMC4864854. Find the plasmids from this publication at ... PubMed PMID: 26344044. PubMed Central PMCID: PMC4666719. Find plasmids from this publication at Addgene...

  14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ... Pubmed PMID: 24733905. Pubmed Central PMCID: PMC4000813. 2. Taylor, Justin Q., et al. “Avoiding a lost... Pubmed PMID: 27175853. Pubmed Central PMCID: PMC4866822. 3. McDowell, Gary S., et al. "Shaping the Future... Pubmed PMID: 25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations...

  15. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ... PubMed PMID: 26344044. PubMed Central PMCID: PMC4666719. 4. Mali, Prashant, et al. "RNA-guided human ... PubMed PMID: 26436575. PubMed Central PMCID: PMC4747789. 6. Platt, Randall J., et al. "CRISPR-Cas9 knockin... PubMed PMID: 25263330. PubMed Central PMCID: PMC4265475. Additional Resources on the Addgene Blog Listen...

  16. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ... PubMed PMID: 26837755. PubMed Central PMCID: PMC4905529. Schmid-Burgk, Jonathan L., et al. “CRISPaint... PubMed PMID: 27465542. PubMed Central PMCID: PMC4974478. Natsume, Toyoaki, et al. “Rapid Protein Depletion... PubMed PMID: 27172193. PubMed Central PMCID: PMC4889673. Additional Resources on the Addgene Blog Learn...

  17. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...44. PubMed PMID: 7597057. PubMed Central PMCID: PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon...95. PubMed PMID: 7624356. PubMed Central PMCID: PMC41457. 8. Schnütgen, Frank et al. 2003. “A Directional... PubMed PMID: 26853302. PubMed Central PMCID: PMC4760889. Additional Resources on the Addgene Blog Learn...

  18. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ... PubMed PMID: 25337688. PubMed Central PMCID: PMC4244833. Kellenberger, Colleen A., et al. "RNA-based ... PubMed PMID: 27446908. PubMed Central PMCID: PMC4923196. Panchapakesan, Shanker Shyam S., et al. "Ribonucleoprotein... PubMed PMID: 25026079. PubMed Central PMCID: PMC4143336. Additional resources on the Addgene blog Learn...

  19. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... PubMed PMID: 25980573. PubMed Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu... PubMed PMID: 26224555. PubMed Central PMCID: PMC4884002. 10. Boer, E. de, Rodriguez, P., Bonte, E., Krijgsveld... PubMed PMID: 25993512. PubMed Central PMCID: PMC4439073. 12. Ludwig, L. B., Hughes, B. J., & Schwartz...

  20. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... PubMed PMID: 26492139. PubMed Central PMCID: PMC4862005. Jiang, Wenyan, et al. "RNA-guided editing of...2015): e83-e83. PubMed PMID: 25800749. PMCID: PMC4513841. Latynski US, Valentovich LN. "DNA tuner: a computer...PubMed PMID: 26002895. PubMed Central  PMCID: PMC4495200. Reisch, Chris R., and Kristala LJ Prather. "...

  21. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...5467. PubMed PMID: 271968. PubMed Central PMCID: PMC431765. 8. Heather, James M., and Benjamin Chain. "The... PubMed PMID: 26554401. PubMed Central PMCID: PMC4727787. 9. Hunkapiller, Tom, et al. "Large-scale and...PubMed PMID:  25743089 . PubMed Central PMCID: PMC4411503. Resources on the Addgene Blog Learn More about...

  22. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ... PubMed PMID: 26538064. PubMed Central PMCID: PMC4633726 Find SAM plasmids used in this publication at... PubMed PMID: 26939770. PubMed Central PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids... PubMed PMID: 26035283. PubMed Central PMCID: PMC4649911. Khalili K, Kaminski R, Gordon J, Cosentino L...

  23. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ... PubMed PMID: 25492562. PubMed Central PMCID: PMC4351462. 8. Ousterout DG, Kabadi AM, Thakore PI, Majoros... PubMed PMID: 25692716. PubMed Central PMCID: PMC4335351. 9. Ran FA, Cong L, Yan WX, Scott DA, Gootenberg... PubMed PMID: 25830891. PubMed Central PMCID: PMC4393360. Additional Resources on the Addgene Blog Learn...

  24. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... PubMed PMID: 27465542. PubMed Central PMCID: PMC4974478. 3. Schaub, F.X., et al. (2015) Fluorophore-NanoLuc... PubMed PMID: 26424696. PubMed Central PMCID: PMC4668208. 4. Chu, J., et al. (2016) A bright cyan-excitable... PubMed PMID: 27240196. PubMed Central PMCID: PMC4942401. 5. Susuki, K., et al. (2016) Five colour variants...

  25. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ... PubMed PMID: 26211958. PubMed Central PMCID: PMC4663986. 2.  A. M. Singh, V. V Adjan Steffey, T. Yeshi... PubMed PMID: 26702451. PubMed Central PMCID: PMC4686154. 3. A. M. Singh, D. W. Perry, V. V. A. Steffey... PubMed PMID: 26299960. PubMed Central PMCID: PMC4559351. 8. A. G. Uren, H. Mikkers, J. Kool, L. van der...

  26. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...PubMed PMID: 25075903. PubMed Central PMCID: PMC4486245.2. pXPR_011 (Plasmid #59702) was a gift from John... PubMed PMID: 25184501. PubMed Central PMCID: PMC4262738. 3. EGFP gRNA (BRDN0000561167) (Plasmid #80034... PubMed PMID: 26780180. PubMed Central PMCID: PMC4744125. Additional Resources on the Addgene Blog Genome-wide...

  27. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ... PubMed PMID: 21527931. PubMed Central PMCID: PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and... PubMed PMID: 25884622. PubMed Central PMCID: PMC4401519. 13. Auffray, Cedric, et al. "Monitoring of blood... PubMed PMID: 26755602. PubMed Central PMCID: PMC4743831. 17. Cavanagh, Lois L., and Wolfgang Weninger...

  28. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... PubMed PMID: 26780180. PubMed Central PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng... PubMed PMID: 25075903. PubMed Central PMCID: PMC4486245. 4. Ma, Hongming, et al. "A CRISPR-based screen... PubMed PMID: 26190106. PubMed Central PMCID: PMC4559080. 5. Tzelepis, Konstantinos, et al. "A CRISPR ...

  29. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...-659. PubMed PMID:18727145. PubMed Central PMCID:PMC4007248. 11.Hess, Samuel T., Thanu PK Girirajan, and...-218. PubMed PMID:25581799. PubMed Central PMCID:PMC4344411. 13.Berlin, Shai, et al. "Photoactivatable...-858. PubMed PMID:26167640. PubMed Central PMCID:PMC4597790. 14.Wang, Yingxiao, John Y-J. Shyy, and Shu...

  30. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ... PubMed PMID: 24336571. PubMed Central PMCID: PMC4089965. 2. Sanjana, Neville E., Ophir Shalem, and Feng... PubMed PMID: 25075903. PubMed Central PMCID: PMC4486245. Resources on the Addgene Blog Browse Our CRISPR...

  31. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... PubMed PMID: 24531762. PubMed Central PMCID: PMC4697943. Find plasmids from this publication at Addgene... PubMed PMID: 25925567. PubMed Central PMCID: PMC4446448. Additional Resources on the Addgene Blog Read...

  33. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...(6228) (2015): 1367-71. PMID: 25792328 PMCID: PMC4451088 Find plasmids from this paper at Addgene. 3... 165(4) (2016): 976-89. PMID: 27153498 PMCID: PMC4889334 Find plasmids from this paper at Addgene. Additional...

  34. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ... PubMed PMID: 26808669. PubMed Central PMCID: PMC4916159. 2. Jacobson, K., Rajfur, Z., Vitriol, E., & ... PubMed PMID: 18706812. PubMed Central PMCID: PMC4445427. 3. Bulina, M. E., Chudakov, D. M., Britanova...

  35. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... PubMed PMID: 25497837. PubMed Central PMCID: PMC4383097. 3. Kim, Sojung, et al. "Highly efficient RNA-guided... PubMed PMID: 24696461. PubMed Central PMCID: PMC4032847. Resources on the Addgene Blog Brush Up on Your...

  36. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    .... PubMed PMID: 8491198. PubMed Central PMCID: PMC413431. 2. Bukrinsky MI, et al. “Active nuclear import...6584. PubMed PMID: 1631159. PubMed Central PMCID: PMC49545. 3. Coffin J, Hughes S, Varmus H. Retroviruses...

  37. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ... PubMed PMID: 25775042. PubMed Central PMCID: PMC4414919. 5. Yarmolinsky, Michael B., and L. Gabriel. ... PubMed PMID: 24458642. PubMed Central PMCID: PMC4121734. Additional Resources on the Addgene Blog Fluorescent...

  40. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... PubMed PMID: 26837755. PubMed Central PMCID: PMC4905529.  Schwartz, M. L., & Jorgensen, E. M. "SapTrap... PubMed PMID: 26552885. PubMed Central PMCID: PMC4689222. Additional resources on the Addgene blog Learn...
Showing: 1 - 40 of 126 results