We narrowed to 222 results for: Mac

...bias observed in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed...primary cells Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific...rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human...2023). They found the best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier...to identification and characterization of AAV.CAP-Mac. Image reproduced from Chuapoco, Flytzanis, Goeden...2023, under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R., Flytzanis, N.C.,...

... our paper, “Synthetic recombinase-based state machines in living cells” (1), into a more straight-forward...our engineered devices, recombinase-based state machines for your own experimental needs. For more context...the paper. Why might one be interested in state machine technology? Biological research has produced a ...timing of these factors. Recombinase-based state machines (RSMs), which take on a particular DNA-sequence...terminators, etc) are composed. Fear not – the state machine design space is highly degenerate and often times...Roquet et al., Synthetic recombinase-based state machines in living cells. Science. 353, aad8559 (2016).... recombinase-based state machines...

...ab1 chromatogram file 4Peaks (Mac) SnapGene Viewer (Mac/PC) FinchTV(Mac/PC) Chromas (PC) 2. You should...

...web and desktop version that works with Windows, Mac, and Linux operating systems.  Zotero  Zotero is ...is a desktop based program that’s compatible with Mac, Windows, and Linux.  Mendeley and Zotero have a ...

...is magnetic bead-activated cell sorting, or MACS. But MACS usually only allows for sorting based on a ...achieves greater purity of your sorted cell sample than MACS. An effective strategy to sort out your cells of...

...This post was contributed by guest blogger Sean Mac Fhearraigh, co-founder of www.assaygenie.com. No ... cycle.  Many thanks to our Guest Blogger, Sean Mac Fhearraigh! Seán was a PhD student at University...

...al, 2023 AAV.CAP-Mac CNS: neurons (rats, infant green monkey, rhesus macaque); vasculature (adult...Old World monkeys AAV.CAP-Mac is largely neurotropic in infant rhesus macaques and green monkeys (Chuapoco...caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid, developed and deposited by ...selected from a library screened in marmosets. AAV.CAP-Mac effectively transduces vasculature in adult marmosets...astrocytes (Chuapoco et al., 2023). Additionally, AAV.CAP-Mac has a lower transduction rate than AAV9 in the liver... rhesus macaques (Chen et al., 2022). AAV.MaCPNS2 is slightly more effective than AAV.MaCPNS1 at transducing...rats); CNS neurons (infant rhesus macaques) Mice, rats, rhesus macaques No (mechanism unknown)   Chen...

...source and available for desktop use for Windows, Mac OS X, and Linux. Inkscape is versatile and has a ...

...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well...death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career ...

... Pubmed PMID: 3325793 Schumacher MA (2012) Bacterial plasmid partition machinery: a minimalist approach...origin of replication and the replication control machinery (Need a refresher on the Origin of Replication...they compete for the same replication control machinery within the cell. Most plasmids encode a negative...through inhibiting translation of replication machinery proteins, or directly inhibit replication by binding...origin of replication and blocking replication machinery (del Solar, 1998). As the copy number of the plasmid... structure that physically blocks replication machinery when plasmid copy number is high (del Solar et...replication of each plasmid using unique replication machinery. However, when plasmids have the same origin of...

...and most machines have a protocol that automatically performs the normalization step.  Machines Most general-use...different brand of qPCR machine, especially if you’re using a rapid-cycle machine such as a LightCycler,...previously-generated validation data, be aware that different machines, samples, and treatments may affect the assay....running a multiplex reaction or on a rapid cycle machine. Melt Curves An advantage of Sybr chemistry is ... by bubbles in wells, variations in volume, or machine surge errors and allow for well-to-well normalization... likely need to re-optimize protocols for that machine. (Teo, et al, 2002; see paper for more information...information on optimizing for LightCycler machines.) Multiplexing It is possible to multiplex qPCR when using...

...that you come into the lab wishing there was a machine that could make your life a hundred times easier...off our hands. Good news! There is probably a machine or robot out there that does exactly what you want...lot of time. Enter the Biomek FXP! We wanted a machine that would improve our transformation protocol ...played a factor in our decision to go with this machine, but we also really liked that Beckman was flexible...apart before plating. With this fine piece of machinery we have been able to transform 96 samples in about...lab team. Enter the Liquid Handler!     This machine fits on a table top and can be easily programmed...t electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful...

...Thanks to our guest blogger Cameron MacPherson! Cameron R. MacPherson is Lead Data Scientist for the Milieu...post was contributed by guest blogger Cameron MacPherson at the Institut Pasteur CRISPR software and the...such as ApE or commercial workbenches such as MacVector or Benchling. Utility functions: These functions... can also download the software for use on any machine with OpenCL enabled hardware. This OpenCL dependency...genomes), then it is worthwhile buying a dedicated machine. On the offline front, there is a Python script...Pasteur, Paris. You can follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al....

...code of life. In 1944, Oswald Avery, Colin MacLeod, and Maclyn McCarty’s experiment debunked the myth that...to stars in space ships and the rise of killer machines that exterminate entire humanity were the two ... technologies rely on the cellular DNA repair machinery, which can lead to high frequency of indel formation... the use of molecular dynamics simulations and machine learning to develop a predictive understanding ...://doi.org/10.1038/s41586-019-1711-4 Avery OT, MacLeod CM, McCarty M (1944) STUDIES ON THE CHEMICAL NATURE...

...experiment. Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in... cell. These so-called recombinase-based state machines could potentially be used to report upon and/or...post to learn more about recombinase-based state machines and how they function. Adapting Toehold Switches... describes R-bodies, “force generating protein machines that can extend and retract over many microns ...

... CN, Yang KK, Robinson JE, Mackey ED, Gradinaru V, Arnold FH (2019) Machine learning-guided channelrhodopsin... Arnold’s lab and Viviana Gradinaru’s lab used machine learning to design new channelrhodopsins that are...channelrhodopsin that’s successful in vivo. Using machine-learning to overcome channelrhodopsin design challenges...challenges, the Arnold and Gradinaru Labs developed a machine-learning model to help them construct “designer...

... passes through the machine’s lasers and gives off a signal read by the machine’s sensors. (Usually this...or unbound antibodies.) Each event read by the machine is plotted as a dot on an X,Y axis to generate ...variations in fluorescent signals, and how the machine reads/interprets the signals, the axis is instead...populations. This shape is called a ‘gate’; it tells the machine that everything inside the gate is one population...

...your macromolecule of interest To utilize the natural binding of biotin and avidin, macromolecules of interest...process by which biotin is added to a protein or macromolecule. Biotin can be added enzymatically or chemically...soluble molecule that does not interfere with the macromolecules it is added to. Biotin also has an exposed ...developed several methods to chemically biotinylate macromolecules of interest both in vitro and in vivo. Biotinylation...

...therapeutic proteins. European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft...further differentiated into dentritic cells or macrophages through cytokine induction (Groell et.al, 2018...Immunotoxicology Testing of Monoclonal Antibodies in Macaca fascicularis: From Manufacturing to Preclinical...

...The most commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as ...the system. Since then, multiple biotech and pharmaceutical companies (along with some research laboratories...the expression of recombinant proteins by the pharmaceutical and biotech industries as a protein expression...specifically glycosylation. The native glycosylation machinery of K. phaffii lacks certain terminal mannose glycopeptides...

...Hippel-Lindau (VHL) to bring the endogenous degradation machinery into close proximity with the target protein, ...degraders provide distinct advantages over pharmacological inhibitors including rapidly depleting a protein-of-interest...to co-opt the endogenous ubiquitin proteasome machinery to degrade any protein. We are particularly excited...FKBP12F36V-fusion and the ubiquitin proteasome machinery through binding with cereblon, leading to rapid...

...Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine...et al., 2019). Using PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed...be specifically and potently activated by pharmacologically selective effector molecules (PSEMs). Higher...

... efficiency and building prediction tools with machine learning (PRIDICT, PRIDICT2.0).   How to apply...chromatin-based prediction tool (ePRIDICT). In summary, machine learning-based prime editing prediction models ...computational biology. His expertise includes applying machine learning techniques to make sense of biology, with...B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction of prime editing efficiency...

... will be recognized by its host’s replication machinery. A shuttle vector designed for unrelated bacteria...requires compatible cell lines that express viral machinery. Alternatively, some slow-growing cells, particularly...nutrient. The vector provides the missing synthesis machinery, and the cells are switched to minimal media, ...transfection into producer cells, which have the machinery to manufacture infectious viral particles. Those...

...oncology, virology, immunology, microbiology, and pharmacology (many of which are represented in Addgene's ...cell culture over the last few decades some pharmacologists have tended to avoid cell culture in favour...experience through working for a variety of pharmaceutical companies including GSK, AstraZeneca-MedImmune...talks and seminars on cell culture and other pharmaceutical topics. The LGC Group also provides standards...

...., & MacRae, I. J. (2009). The RNA-induced Silencing Complex: a versatile gene-silencing machine. Journal... plasmids, shRNAs hijack the endogenous miRNA machinery for processing (Figure 1). The shRNA sequence ...the bound miRISC can simply block translation machinery. The composition of the RISC varies, but at the...

...typical PCR machines, the authors use affordable, field-ready, Bluetooth-enabled PCR machines that can even...approximately 1-2 hours Equipment needed: Mini PCR machines by DIY-Bio, P51 cardboard fluorescence visualizer...mentioned, likely ~1-2 hours Equipment needed: PCR machines/Heat blocks, Lateral flow strips, Agarose gel ...

...greater than the one that can be achieved using pharmacological or electrophysiological methods (Deisseroth...depression-like behaviors: a critical analysis." Pharmacology Biochemistry and Behavior 122 (2014): 144-157...circuits in response to drug exposure." Neuropsychopharmacology 41.10 (2016): 2424. PubMed PMID: 26934955...applications of optogenetics." Current protocols in pharmacology 75.1 (2016): 11-19. PubMed PMID: 27960028. Van...

...phagocytosis (ADCP), Fcγ receptors on macrophages bind IgG antibodies. The macrophages then engulf and digest the...antibody-dependent cellular phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171...

...chromosomal DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally...lower temperatures and allows the replication machinery room to come in and get busy making copies.   ...incompatible because they will compete for the same machinery, creating an unstable and unpredictable environment...

...organelles, such as mitochondria, as well as macromolecules like actin and myosin, and enzymes for transmitter...two pathways also use different cytoskeletal machineries to facilitate transport: retrograde transport...interaction with uptake or intracellular transport machinery. Addgene offers viral preparations of AAV2-retro...serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex, Neurosci Res 93, 144-157. PubMed...

...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...intended edit further weakened recognition by MMR machinery and improved editing efficiency. The lab also ...

...Bhang, a research investigator in the In Vivo Pharmacology group at Novartis Oncology, is an exciting new...her new role as an investigator in the In Vivo Pharmacology group at Novartis Oncology, Bhang has been working... Bhang is a research investigator in In Vivo Pharmacology at Novartis Oncology and is particularly interested...

... which genes should be ‘read’ by the cellular machinery. This is how most cells in the body contain the...compacted it becomes. This prevents the cellular machinery from physically interacting with the genes in ... transposon methylation underlies the mantled somaclonal variant of oil palm." Nature 525.7570 (2015):...

... it has been challenging to introduce CRISPRi machinery into diverse bacteria species. Existing protocols...bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient...contain a chromosomal copy of the RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the...

...and departments will have qPCR machines available for use. ddPCR machines are not as commonly available.... PMID: 21554069. Haridan, U. S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., Shueb, R. H., Zaid, ...

...doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing cells....convert adenine to inosine, which the translational machinery treats like guanine. This RNA editor would permit...as assessed using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral...

...This post was contributed by Rory Macneil, founder of Research Space. There are many types of electronic...equal. Many thanks to our guest blogger, Rory Macneil!  Rory is founder and CEO of Research Space, which...in Edinburgh. You can follow Rory on Twitter @rory_macneil.     Additional resources on the Addgene blog...

...temporal control than is achieved by traditional pharmacologic methods. A number of strategies have been developed...where to activate the pathway than traditional pharmacology techniques allow. Multiple photoswitches can...doi.org/10.1038/nn.2495 Marshel, J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja...

... Modification. Pharmaceutics, 13(5), 750. https://doi.org/10.3390/pharmaceutics13050750 Murlidharan, G...serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. Neuroscience Research, 93, 144...

...orchestrating blood-vessel formation. Precise, real-time pharmacology of this target has been hampered by transient.... G., Otto, P., Zimmerman, K., Vidugiris, G., Machleidt, T., Robers, M. B., Benink, H. A., Eggers, C. ...complexes in living cells. British Journal of Pharmacology, 178(12), 2393–2411. https://doi.org/10.1111...

...resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can...initial state of the dsRNA upon activating the RNAi machinery (long dsRNA, typically hairpin RNA, or artificial...-2005-837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based ...

...2. Fluorescence Activated Cell Sorter (FACs) A machine that sorts cells according to whether or not they... deflect them into appropriate containers. The machine is useful for distinguishing cell populations that...PMCID: PMC2569002. 3. Egen, Jackson G., et al. "Macrophage and T cell dynamics during the development and...

...were included in the CGBE. They also developed a machine learning model capable of predicting editing outcomes... CRISPRi screens, target-library analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425...with sequence context preference predictable by machine learning methods. Nature Communications, 12(1),...

...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis (@AlexDainis...

...contamination, but consider flow cytometers. These fluidic machines handle biological samples and are cleaned between...between each use. You wouldn’t throw away your FACS machine after a single use. So how about washing and reusing...

...non-academic people.” Daniel went on to discuss his work: “Macro photography has been my passion for a while - a ...teacher of dental radiography, had focused his x-ray machine not on teeth, but on blossoms, thus revealing the...

...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme...protein engineer currently working at Sanofi Pharmaceuticals in Cambridge, MA. His work experience is in...

...Department of Pharmacology, I taught a couple of lectures in the undergraduate pharmacology course, like...

...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This...