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We narrowed to 116 results for: Tub;

Showing: 1 - 50 of 116 results

  2. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...a 2D barcoded tube (see next section) and every time something is removed from this tube, the barcode ...contained within the tube, but more than that, they also help us keep track of the tubes’ physical location... 2D barcode and LIMS: Barcoded Tube Plate Freezer Rack Freezer Tubes are assigned a location within ...because every barcode on every tube contains information about that tube’s appropriate location. The barcode...each individual tube.  If a plate of samples is ever dropped, we can simply scan the tubes and know what..., we prepare three sets of 2D barcoded tubes. Two of the tubes are used for long term storage (one is ... the sample tubes that we use match those of the appropriate deposit. Using barcoded tubes is part of ...

  3. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ... of tubes’ o-rings are, in fact, permeable to CO2 at low temperatures (-80°C) and once in the tube, the...What we did next: Venting the tubes We then tested whether venting the tubes prior to thaw released the ... and then vented the tubes by completely removing the caps, incubating the tubes in an upright position...and then recapping the tubes prior to thaw. We compared vented and non-vented tubes to controls that were...returning the tubes to the -80°C freezer after storage on dry ice allowed the CO2 to leave the tubes, and would...thawed the tubes after the indicated incubation periods, and measured the pH. We found that tubes subjected...therefore devised a few experiments to determine if our tubes were permeable to the CO2 released from dry ice,...

  4. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...recruited to microtubules under blue light, removing tubulin dimers from the microtubule body. B) Schematic...  Opto-katanin: a light-powered microtubule-cutting sword Microtubules are one of the most important components... several strategies are available to disrupt microtubules in cells, they have various disadvantages and...and limitations – for example, microtubule-destabilizing drugs are impossible to target to specific subcellular...published a new optogenetic tool for localized microtubule disassembly, Opto-katanin (Meiring et al., 2022...Figure 1: Opto-katanin induces fast, localized microtubule severing. A) Schematic of Opto-katanin constructs...under CC-BY 4.0 license. A naturally occurring microtubule-severing protein, Katanin is of course named ...

  5. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    .... It is an advanced technique, as it requires intubation of the mice, which can be tricky given their ...though drug delivery is also common. Due to the intubation, this procedure is considered quite invasive....Restraint: anesthesia Location: trachea Gauge (tracheal tubing): 23–20 Volume: < 0.07 ml Skill required: advanced...involves administering medication through a feeding tube with a bulb on the end, which prevents accidental...accidental tears or punctures to the throat. The feeding tube can be flexible or metal, depending on researcher...scruffing Location: throat Gauge (bulb-tipped feeding tube): 18–20 Volume: up to 10 ml/kg Skill required: intermediate...Expert Intratracheal Anesthesia Trachea 20–23 (tubing) < 0.07 mL Advanced Intranasal Anesthesia Nose...

  6. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... new tube. In the first example we’ve used inheritance to tightly couple the contents of a tube with ...with the tube itself. If something happens to the worm that requires a change of tube, there’s no clean way...second example we have a separate worm model and tube model, we can interchange them easily as needed....or a freezer. Some are disposable, like a plastic tube. Talk with your lab team about how they would like...

  7. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...vials with a specific amount of agar. There is a tube that goes from the needle to the flask with agar...dispense and you’re on your way. Label your tubes with a label maker. Label makers generate far more...your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is a simple table...roller to go over caps and push them into micronic tubes instead of pushing them in one by one. It seems ...instance, vial labeling would be much easier with a Tube Writer, which air brushes the information onto the...vials instead of requiring us to put a label on each tube by hand. We’ll be sure to tell you about more lab...

  8. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...Mycobacterium tuberculosis resistance to pyrazinamide (PZA) (a drug commonly used to treat tuberculosis) and in... drug resistance mechanisms of Mycobacterium tuberculosis Dr. Patricia Sheen, Universidad Peruana Cayetano...detection of PZA-resistance is necessary to improve tuberculosis care and control. In her current research, Sheen...improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies biofilm formed...

  9. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...terms of total view count and videos published, YouTube reported a total of 865,995 views for the 926 video...  Check Out Addgene Videos Over at the Addgene Youtube Channel   After watching these trends for a few...these platforms were developing, Google acquired Youtube and has been growing its online video market ever... hosted on the platform, they can be hosted on YouTube, Vimeo, and other platforms (such as university...current-biology/abstract/S0960-9822(16)30873-9 Youtube: https://youtu.be/EHHhiawIdWM Saving salmon, one...

  10. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...add the various reagents to a single tube and simply flick the tube several times. This will help ensure...processes including automated vial filling, accurate tube-labeling, quick plate pouring, and much more. We...quick trick. Just flip, and press! Lab tip #2: Tube flick trick When combining reagents in the lab, ...

  11. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ... Plasmids! Light controls the activity of a microtubule-associated protein to regulate cell growth dynamics...light by modifying EB1. EB1 normally sits on microtubules to attract other proteins involved in their ...into two halves (Fig. 1): one containing the microtubule binding domain and the second containing the ...modified EB1 protein are overexpressed in cells, microtubule growth can be switched off with blue light which...

  12. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... Limit tube transfer - the fewer opportunities your viral particles have to stick to your tubes, the higher...siliconized tubes to store samples as it is more difficult for viral particles to adhere to these tubes. Rinse...

  13. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...Streptococcus pneumoniae. Robert Husson lab's M. tuberculosis CRISPRi plasmids A group from the Husson lab... used CRISPRi to study essential genes in M. tuberculosis. dCas9 and a gRNA are expressed on pRH2502 and... (Singh et al. 2016). Sarah Fortune's lab M. tuberculosis CRISPRi plasmids Noting that the more common... for transcriptional silencing Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition to ...Investigating essential gene function inMycobacterium tuberculosisusing an efficient CRISPR interference system. Nucleic...

  14. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...our guest blogger Kutubuddin Molla from the Pennsylvania State University Kutubuddin Molla is an Ag-biotechnologist...This post was contributed by Kutubuddin Molla, a Fulbright Visiting Scholar at the Pennsylvania State... Institute, Cuttack, Odisha, India. Currently Kutubuddin is a Fulbright Visiting Scholar at the Pennsylvania...

  15. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ...way! Many thanks to guest blogger Kaustubh Kishor Jadhav! Kaustubh Kishor Jadhav, MSc is a Research Assistant...This post was contributed by guest blogger, Kaustubh Kishor Jadhav, a Research Assistant at MGMs Institute...lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain in the...

  16. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...used to convey the difference between a gradient tube before and after ultracentrifugation so that a scientist...each layer. The right panel illustrates a gradient tube before and after ultracentrifugation. The viral ...the bevel pointed up. A syringe at the top of the tube allows air to enter the column so the virus will...

  17. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...method procedure The procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant...are mixed with 3 ml of 2X DMEM in a 50 ml sterile tube (for 60 mm dishes only 1.5 ml of each are used)....agarose plug by rapid pipetting, as shown in the YouTube video. Before returning the plate to the incubator...

  18. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ... K, et al (2015) Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun 6:7933. https... start re-conceptualizing actin filaments or microtubules. This issue is shared by other SRM techniques...

  19. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...growing tips of microtubuli and plasmids tagged with MapTau to label entire microtubules. Find these fluorescent...best motif was from the Lck gene. For marking microtubules of mammalian cells, the author found that too...

  20. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel, 2023. ...to protocols. In 2013, we launched the Addgene YouTube channel to add a strong visual component to our...

  21. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...same tube. You can clone up to 4 DNA fragments, in a specific order and orientation, in one tube, into...possible to set up the BP and LR reactions in the same tube, speeding up the cloning of the attB-PCR products...

  22. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ... — check out Addgene’s new Quick Tip series on YouTube! In this series, we share quick tips to help you...video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips, protocols, animations, ...

  23. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation...video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog...

  24. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...to sit down and talk with us. Tom McFadden is a YouTuber and science educator looking for creative ways...communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com.   Special thanks...

  25. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ...right, researchers of the world. Cap your Eppendorf tubes, hang up your pipettes, and seal up those plates...decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing routine...

  26. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ... safely recycle these materials either.  Test tubes aren’t cheap everywhere Lab supplies and equipment...Coast where her first job was to wash Eppendorf tubes which were to be used for 3 months before they could...

  28. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...chemical fuel sources. Like an actin filament or a microtubule, R bodies are a polymer of many copies of monomeric...ectosymbionts of Euplotidium (Ciliophora) that contain microtubule-like structures are bacteria related to Verrucomicrobia...

  29. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    Published
    Feb. 8, 2022, 2:15 p.m.
    ...time needed to prep for an experiment (labeling tubes never takes longer than 5 minutes, right?) As a ...   Visual Planning  Plate maps, pre-labeling tubes, and even drawing out experimental steps are worth...

  31. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...many different things do you simply refer to as a “tube”? We took steps towards solving this problem by...Prescribing an official name to all of our plates, tubes, and racks meant we had fewer errors in our LIMS...

  32. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...synthetic biologists to perform large scale, one-tube cloning reactions. In this blog, we’ll cover the...assemble multiple DNA fragments together in a one-tube reaction. Even though most MoClo reactions use Golden...

  33. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...prescribed order. These fragments can be pooled in one tube and ligated in as little as 30 minutes! Alternatively... per cloning reaction drops dramatically, as one tube of enzyme should be enough for at least 50 cloning...

  34. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase the...desired density. The volume of your flask or culture tube should be at least four times greater than your ...

  35. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ..., mastered the art of finding how-to videos on YouTube long before he learned (somewhat successfully) ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter, for...

  36. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin Repeatable, directional cloning   The ...

  37. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Antibodies may even aggregate just from dropping the tube they are contained in (Randolph et. al., 2018). ...aliquoting and storage process to prevent dropped tubes. Now that your antibody is produced, harvested, ...

  38. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...dilutions. Aliquot all of your reagents into single-use tubes and grab a fresh one for each set up.  Wipe down... al. "Detection of circulating Mycobacterium tuberculosis-specific DNA by droplet digital PCR for vaccine...

  39. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing a new DNA fragment...Watson, and Ingo H. Greger. "IVA cloning: a single-tube universal cloning system exploiting bacterial in...

  40. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...matching system that follows the sample from the DNA tube all the way to the cleared viral preparation pool...ensuring the identity of each sample from the DNA tube all the way to the viral preparation. After production...

  42. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...components into plants. Nanoparticles (carbon nanotubes, Kwak et al., 2019), DNA nanostructures (Zhang... using chitosan-complexed single-walled carbon nanotube carriers. Nat Nanotechnol 14:447–455 . https:/...

  43. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...buffer called sheath fluid and transported through tubes or capillaries to a laser. As the cells move through...positive for a desired parameter is collected in a tube or plate, while a droplet containing a negative ...

  44. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...plastic in spin columns and buffer bottles Waste tubes are made of 100% post-consumer recycled plastic ...buffers and columns sold separately Some, waste tubes sold separately No Other Minimized amount...

  45. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...with all of the plasmids in the library in a single tube. The number of unique plasmids in a pooled library... submitted as 15 µL of DNA in a 1.5 mL microfuge tube at a concentration of 0.1–1 µg/µL (or bacterial ...

  46. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... beta BFP-KDEL Rtn4a-GFP (tubular ER) Microtubules mCherry-alpha-tubulin Early Endosome Vacuolar...

  47. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...found in mammals.      Subscribe to Addgene's YouTube channel for more helpful animations, useful protocol...

  48. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...complete collection of protocol videos on the Addgene YouTube page. ...

  49. New Western Blot Protocol Video!

    Type
    Blog Post
    Published
    March 28, 2023, 1:15 p.m.
    ...Protocol page, and find our protocol videos on our YouTube page.     ...

  50. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ...account, or you can watch the full animation on our YouTube channel.  More resources on the Addgene blog Antibodies...
Showing: 1 - 50 of 116 results