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We narrowed to 39 results for: cas12

Showing: 1 - 39 of 39 results

  1. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

    Type
    Blog Post
    Published
    Nov. 29, 2018, 1:53 p.m.
    ...version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But what is the function...indiscriminately cleave ssDNA in trans like Cas13 and Cas12 do for RNA and dsDNA, respectively. However the ...specific in its recognition of ssDNA than Cas13 or Cas12 are for the other types of nucleic acids and required... has been created by the authors. This kit uses Cas12 and Cas13 to quickly detect the presence of infectious...dsDNA, and now ssDNA. By comparing the ability of Cas12 and Cas14 to detect SNPs using the DETECTR kit, ...

  2. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...V Type V uses Cas12 effectors and is one of the most popular alternatives to Cas9. Cas12 is unique because..., each relying on a unique Cas effector — Cas9, Cas12, or Cas13. If you’d like to explore Class 2 CRISPR...multiplexing. The double-stranded breaks caused by Cas12 create short 3’ overhangs, which leads to slightly...type V, named A–I and U. V-A is the most popular Cas12 effector, but others have become more notable in...

  3. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...large deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference...single mismatch in the target sequence Similar to Cas12, it is also significantly smaller in size than traditional...generates staggered breaks, also known as sticky ends. Cas12a cleavage results in 5 bp overhangs and is particularly...DNA generation, such as homology directed-repair. Cas12a is also conducive for targeting AT-rich genomic...shared by other Cas enzymes outlined below as well. Cas12a also has significant therapeutic promise due to...pre-crRNA into crRNA). The size and simplicity of Cas12a makes it easier to package for therapeutic delivery...cutting efficiency and diversify PAM sequences of Cas12a to further optimize it for therapeutic and laboratory...

  4. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...delivery was much better with Cas12, which uses a much shorter crRNA. Cas12-RNP-PAGE achieved high editing...

  5. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ...TnpBs are thought to be evolutionary ancestors of Cas12 proteins. Functionally, while Cas9 contains two ... A. (2024). A miniature alternative to Cas9 and Cas12: Transposon‐associated TnpB mediates targeted genome...-known CRISPR-associated nucleases are Cas9 and Cas12a, the heavyweights in the genome editing toolbox...acids in size. To give a visual analogy: if Cas9 or Cas12a is a soccer ball, TnpB is a baseball — much smaller...two nuclease domains (HNH and RuvC-like), and Cas12a has one (RuvC-like), TnpB also operates with a single...scissors" (one for each strand), while TnpB, like Cas12a, uses just one to cleave both strands. Similar ...transposon-associated motif), which is positioned like Cas12a PAM sites. For Cas9, the PAM is located just downstream...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology...SARS-CoV-2 sequence detection method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/...of SARS-CoV-2. If the Cas12a-sgRNA complex binds to the target sequences, Cas12a activity gets activated...Unlike Cas12a, Cas13a does not require strict sequence preferences at the target site, while Cas12a requires..., check out this table.  All-in-one dual CRISPR/Cas12a (AIOD-CRISPR) assay – for rapid, ultrasensitive...reaction system and incubated at one temperature. Two Cas12a-crRNA complexes are designed, each binding to one...cleavage. Upon amplification by the RPA primers, the Cas12a-crRNA binds to the complementary region in the ...

  8. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ... for SCD in 2022 and Editas Medicine starting a Cas12-based clinical trial for SCD in 2022 as well. Hereditary...

  10. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Guevara H, Wadford DA, Chen JS, Chiu CY (2020) CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology... RPA. When Cas12a-cRNA base pairs with the dsDNA of interest, the DNase activity of Cas12a is initiated...utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop simple, portable, and inexpensive platforms... 2017 and 2018). While the Doudna lab has used Cas12a’s non-specific ssDNA degradation to develop the ...degradation of neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively, to cleave and activate a reporter...reaction, Cas enzymes, such as variations of Cas13 and Cas12a, are combined with different fluorescent reporters...DNA Endonuclease Targeted CRISPR Trans Reporter Cas12a (Cpf1) is a CRISPR Cas variant that can also cleave...

  12. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... engineered from the uncultivated archaea Cas12f (Un1Cas12f1, or Cas14a1) system. The CasMINI system was...engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of Cas12f. The CasMINI...system starting with the same Un1Cas12f1 (Cas14). They noted that Cas12f1 had an extra-long gRNA for its...expression by fusing dCas12f to VP64. Read the paper in Nature Biotechnology Find the Cas12f-GE plasmids Programmed...that utilizes a Cas12f from a different species, Acidibacillus sulfuroxidans, AsCas12f1. They found that...activation or gene editing. The more recently identified Cas12f systems from uncultivated archaea are much smaller...system is less than half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging...

  13. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...screening toolkit by optimizing a Cas12a-based method for screens. Cas12a’s ability to perform multiplex editing...potential of Cas12a genetic screens by optimizing enzyme activity using an enhanced Cas12a variant (enAsCas12a...genes using Cas12a’s ability to knock out multiple genes in parallel. Lastly, this Cas12a-based screening...either Cas9 or Cas12a. Despite its small size, CasMINI is comparable in efficacy to dCas12a systems in both...targets Optimized genetic screening technique using Cas12a for multiplexed gene perturbation The Penn State...   Optimized genetic screening technique using Cas12a for multiplexed gene perturbation by: Gabrielle... larger expression requirements (Figure 1), but Cas12a has generally been underutilized in genetic screens...

  14. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...different Cas12a inhibitors. Some of these anti-Cas12a proteins have broad inhibitory effects on Cas12a orthologs... research.     CRISPR-Cas12b for human genome editing The best studied Cas12b nuclease from Alicyclobacillus...The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C but...Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature incompatible...Read the paper in Nature Communications Find the Cas12b plasmids  Plant base editing with a Cas9-adenosine...the Mobile-CRISPRi plasmids Identification of Cas12a inhibitors Using a bioinformatics and a screening...orthologs and can block Cas12a-mediated genome editing in human cells. Read the paper in Science Find...

  15. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...first thought to enable MBE by using Cas12a fused base editors (dCas12a-BE). However, the poor editing efficiencies...only minimal payloads. DAP array  Although CRISPR-Cas12a, with abilities to process and release its own ...

  16. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    Published
    Nov. 5, 2019, 1:28 p.m.
    ...for in vivo research and potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019...Strecker, Jonathan, et al. "Engineering of CRISPR-Cas12b for human genome editing." Nature communications...PMC6342934. Teng, Fei, et al. "Repurposing CRISPR-Cas12b for mammalian genome engineering." Cell discovery...

  17. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1), a nuclease that generates double...ends” rather than the blunt ends created by Cas9. Cas12a  displays lower off-target editing than SpCas9 ...Kim et al., 2016).  The Acidaminococcus sp. BV3L6 Cas12a, AsCpf1, uses a 5'-TTN PAM which makes it easier...

  18. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...: efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs. On the sixth day of ...programmed genome editing by a miniature CRISPR-Cas12f nuclease. On the eighth day of CRISPR, Addgene ...

  19. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    Published
    June 10, 2025, 1:15 p.m.
    ... Type V-K CAST systems use a nuclease-deficient Cas12k (Gelsinger et al., 2024). In either type of CAST...systems. A Cas effector (Cascade in Type I systems; Cas12k in Type V-K systems; Cascade shown) combines with...

  20. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...appropriate species. Some Cas proteins, like LbCas12a or AsCas12a, do not require a tracrRNA. sgRNA: “Single-guide...

  21. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...simultaneous gene activation in plants.  FnCas12a, unlike other Cas12a nucleases identified, functions at temperatures...

  22. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...dsDNA target in cis and has no trans activity. AsCas12a, on the other hand, generates staggered DNA nicks... Jinek et al. 2012, or browsing for the latest AsCas12a plasmids, Addgene and CasPEDIA have got you covered...

  23. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Additionally, alternative Cas enzymes exist, such as Cpf1/Cas12a, which recognizes the PAM sequences of TTTV. While... sequence  Additional notes SpCas9 NGG - Cas12a TTTV Great for AT-rich regions Cas9 EQR NGAG...

  24. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ... It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a may promote...

  25. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ... liver.  Two other smaller Cas proteins include Cas12b and CasX. While there are no papers yet published...Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b for human genome editing. Nat Commun 10: .https...

  26. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    .... elegans. In 2017, the Korswagen lab developed Cas12a (Cpf1) for editing in C. elegans (Ebbing et al....motif required by the Acidaminococcus sp. BV3L6 Cas12a (AsCpf1) eases this constraint and allows editing...

  27. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...cancer research. The Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library is a lentiviral library...

  31. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...selections.) CRISPR enzyme (Cas9 [sp], Cas9 [other], Cas12a, RNA-targeting) CRISPR mechanism (KO, CRISPRa...

  33. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...CHyMErA) Knockout Libraries express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and...

  36. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...opt for a completely different Cas effector, like Cas12a (Cpf1). Even more creative options involve generating...
Showing: 1 - 39 of 39 results