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  1. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ... for summer programs. I spent one day at Addgene last summer, through the La Vida Scholars summer program...Figure 1: Addgene's summer interns presenting their capstone projects during the Summer Intern Poster Presentation...I never would have imagined that my summer before high school senior year would have been spent within...Addgene’s internship opportunity taking place next summer.  I applied to Addgene and began the interview...position, but I was waiting to hear back from other summer programs. Amy took into consideration just how ...with exciting news. I managed to secure a virtual summer program, teaching me the basics of physics and ... senior in high school who just completed their summer internship at Addgene. They plan on majoring in...

  2. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...launch this summer! Summer SciComm Series From June-September, we’ll be running a Summer Science Communication... is finally warming up in Boston and that means summer is right around the corner. Here at Addgene, we...inspired by the new seasons, so we've made some big summer plans this year! New Blog Series We have not one...soon! We’ll also be building our video library all summer long, with additions to our other video series.... and warm weather - check out our Instagram all summer long to see what kind of shenanigans we get into...

  3. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...review or curation models where editors’ comments, reviewers’ comments, and author responses can be shared ... the Addgene blog Summer SciComm Series: Modes of Communication Intro to Our Summer SciComm Series!...these issues, by sharing their work publicly and immediately once the manuscript is drafted. Preprints were...for authors to post. They can be assessed and commented on by readers, typically peers in the field, and...on your poster, or invite attendees to read and comment online! Want the general public (say, policymakers...Preprints and their feedback, in contrast, are immediately and openly accessible, allowing problematic work...

  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...anti-GFAP [N206A/8R] antibody shared by James Trimmer’s lab (Figure 5) as well as antibodies for identifying...N206A/8 TC Supernatant). Image attribution: James Trimmer, UC Davis.   ...

  5. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...Welcome to the last of our Summer SciComm Series! We hope you've enjoyed this small glimpse into the ...SSCS: Cognitive Load SSCS: Preprints Intro to our Summer SciComm Series  ...

  7. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...Welcome to our Summer SciComm Series, where we’ll be talking about science communication, best practices... active science communication research.   This summer, we’re making time to talk about my favorite thing...scicomm)! We have so many fun topics planned for this summer, but the foundation of all good communication is...

  8. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...reading your material. For the vast majority of summer internships you are applying to, keep your resume... a solid personal statement.  4. Letters of recommendation wisdom  Reach out to 2-3 individuals in your...personal statement. Make certain to choose your recommenders based on your relationships to the person as...of influence. In other words, the longer the recommender knows you the better, and their leadership status...statement along with the request to help your recommenders deliver a superb and detailed portrayal of yourself...

  9. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...resources on the Addgene blog Summer SciComm Series: Cognitive LoadSummer SciComm Series: Modes of CommunicationIntro...courtesy of Leonardo Ramos.  I enrolled into a summer school course at the University of Otago’s Department...CommunicationIntro to Our Summer SciComm Series!...

  10. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... on the Addgene blog:  Summer SciComm Series: Cognitive LoadIntro to our Summer SciComm Series    ...high Uses visuals, audios, and text. Incredibly immersive and flexibility to visualize and contextualize...

  11. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...We are now at the second post in our Summer SciComm series and it’s time for one of my favorite scicomm...immunologist, a geneticist, a physicist, etc., is an immense task (it takes, ooh, approximately the length of... about the questions you’ve received, read the comments, seek feedback from others, and listen quietly...More resources on the Addgene blog Intro to our Summer SciComm Series...

  12. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...now in the summer of 2020 that the consequences of undermining science are even more immediate and dire ... Marc Zimmer’s recent book, The State of Science: What the Future Holds and the Scientists Making It ... “What is science?” That’s a very big question. Zimmer, a Professor of Chemistry at Connecticut College...If that sounds ambitious, that’s because it is. Zimmer, whose previous books focused primarily on fluorescent...nearly impossible for a career scientist such as Zimmer to introduce any one of these topics without some...hobbies, are unlikely to be surprised by many of Zimmer’s revelations. The issues he discusses have been...understand every other problem. For non-scientists, Zimmer’s observations will likely range from mildly surprising...

  14. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...double-stranded break repair mechanism: MMEJ (microhomology-mediated end-joining)? MMEJ, a form of alternative end-...balance of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect ...stranded breaks are repaired by two rounds of MMEJ. This double MMEJ strategy looks very similar to HDR, but... possibilities MMEJ can enable. References 1. Sakuma, Tatsushi, et al. (2016).  “MMEJ-assisted gene knock-in...Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR gene knock-in,...need for complicated cloning of homology arms. MMEJ: An Introduction There are three primary methods...with insertions and deletions common. In contrast, MMEJ uses regions with 5-25 bp of microhomology flanking...

  16. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...validation of some of these tools created by the Trimmer Lab that will be the first antibodies distributed...constructing the plasmids for expression of mAbs, the Trimmer lab isolated RNA from cryopreserved hybridomas,...can be a threat to future reproducibility. The Trimmer Lab found that in addition to recovering RNA from...lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation by immunofluorescence-immunocytochemistry...subsequently cloned into the expression constructs. The Trimmer lab found that the recognition sequence for the...webpage. Importantly, the methods developed by the Trimmer Lab for construction and validation of R-mAb expressing...with different mAbs in the same experiment. The Trimmer Lab found that they could modify the CH encoding...

  17. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...cases”. Cases present specific operational or commercial challenges or questions that clients face imminently... and help resolve it through well-informed recommendations. Healthcare consulting firms that specialize... health and economic benefits of novel drugs. Commercial Affairs teams make up a large and diverse group...group of professionals running the marketing, commercialization, collaborative, and financial functions associated...associated with the business. Commercial teams routinely work with consulting firms on projects such as... conversations with R&D, Medical Affairs, and Commercial teams, reflecting the need for all parts of a...crucial for the success of a drug’s clinical and commercial future, and yet are incredibly difficult to find...

  18. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...version of the plasmid (Summers & Sherratt, 1984; Summers et al., 1993; Summers, 1998). Multimeric plasmids... replicate at a higher frequency (Summers & Sherratt, 1984; Summers et al., 1993). These larger plasmids...monomers and various forms of multimers (Summers & Sharratt, 1984; Summers et al., 1993). In these cases, screening...strategies for resolving multimers? Let us know in the comments below or contact us at [email protected]. Resources...electrophoresis. BioProcess Int. 2006 Sep;4(8):38-40. Summers D. Timing, self-control and a sense of direction....1046/j.1365-2958.1998.01012.x. PMID: 9767582. Summers DK, Beton CW, Withers HL. Multicopy plasmid instability...10.1111/j.1365-2958.1993.tb01648.x. PMID: 8361350. Summers DK, Sherratt DJ. Multimerization of high copy number...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...Many antibodies will have a recommended blocking buffer and a recommended concentration. That makes the...particularly your primary antibodies. We always recommend using an antibody validated for your target in... are different than the validation assays, we recommend performing your own validation assays. If you’...There are many different lysis buffers available commercially, and most common buffers have recipes available...to preserve specific protein modifications, we recommend doing a little more research into gel chemistry...separation of proteins of different sizes.  Table 2: Recommended gel percentages for various protein sizes ...binding and washes. It’s less sturdy and not recommended for stripping and reprobing, unless you buy specialty...

  20. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... is my experience looking for the perfect job. Summer work experience in cancer research After graduating...would be very competitive, and so I applied for a summer role as a Research Scientist at the Institute of...to gain relevant experience. I applied for this summer position as I felt I needed more practical lab ...study of e2Fb, which is a tumour suppressor. This summer role was incredibly valuable because it was a set... need to explain why I changed jobs. I highly recommend taking on a similar position in your field of ...and enjoyed them, so I applied for a role at a commercial virology testing company. This particular company...companies. It was really exciting to work in a larger commercial company; the pay was good and there was a variety...

  21. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ... or a combination of the two. There are many commercially available options to choose from.  Transfer ...staining or other reversible protein staining immediately after your transfer. If you are expecting low...blockers, and the manufacturer will usually include recommended blocking conditions on the spec sheet. If you... background after following manufacturer’s recommendations, you can try either longer blocking times or...antibody information doesn’t contain blocker recommendations, you may want to try different protein blockers...make up a fresh batch.  Antibodies We always recommend either using an antibody validated for your protein... well. Dilutions Most antibodies come with a recommended dilution and/or range (typically between 1:500...

  22. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...five pillars of antibody validation, and it is recommended to screen multiple candidate antibodies when ... interest when expressed at endogenous levels (Laflamme et al., 2019). CRISPR-Cas9 approaches are commonly... to produce KO cell lines, some of which are commercially available. Various proteomic and transcriptomic...knockout approaches. In these cases, we would recommend a knockdown approach instead. In this approach...individual experiment.   Figure 3: Our recommendations for an approach that may be used to determine... onto the five pillars, with some specific recommendations for how to use these in practice. This ...received in-kind support to produce this data from commercial antibody manufacturers. References and resources...

  23. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...membrane protein targets in as little as 3 weeks (Zimmermann et al., 2020). This selection platform was designed...interest using the Sybody Generation Toolbox and commercially available reagents in three major steps - all...can be randomized for creating the libraries (Zimmermann et al., 2018). Find the Sybody Generation Toolbox...sybodies against membrane proteins. Image from Zimmermann et al., 2018. Ribosome display The Seeger ...members). This selection step involves using a commercial kit for in-vitro translation of the mRNA libraries...identifying unspecific and low-affinity sybodies (Zimmermann et al., 2020). This step of the protocol starts... . https://doi.org/10.1016/j.cell.2020.04.031 Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead...

  24. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...to address the comments to “you” rather than “the authors.” By addressing the comments to “you” I can ... figures). After finishing my section-specific comments, I re-read what I wrote about the paper after ...in the first (and clunky) version of my general comments and adjust them if my opinion changed after this...that there is another human who is reading your comments. Receiving a peer-review is already a humbling...easily check if I would have formulated specific comment differently if I would be speaking to a colleague...young scientists that are reading your peer-review comments will learn from them and propagate this culture...

  25. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ... to lyse the cells to let the virions out. We recommend using a Tris-based lysis buffer and sonication...Grimm et al., 1999), quantitative PCR (qPCR) (Aurnhammer et al., 2012), droplet digital PCR (ddPCR) (Lock...freeze/thaw cycles (Lock et al., 2010). It is recommended that you measure your infectious titer, since...standard labs. When determining titers by qPCR, we recommend using primers targeting the viral genome or inverted... quantitation blog post.  We generally do not recommend using ELISA-based titering assays, since those...August 2023 References and resources  References  Aurnhammer, C., Haase, M., Muether, N., Hausl, M., Rauschhuber...10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. (2004). Infectious titer...

  26. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...depositor recommendations to grow a plasmid at 30℃ or room temperature. These recommendations also often...strain. For most applications, Addgene uses and recommends DH5𝝰! This strain consistently gives good, high...degradation of your prep. Many plasmid prep kits recommend performing an extra wash step to remove endonucleases...common lab E. coli strains on the blog, and also recommend this handy guide from OpenWetWare for a more extensive...plasmid prepping. Be sure to stay within the recommended range of the filter or you might inadvertently...cultured in media containing half the normally recommended antibiotic concentration. Add supplements to ...

  27. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...Lader Commercial DNA Ladder Penn State Ladder: Homemade Plasmid Prep Penn State Ladder: Commercial Plasmid... 1 kb DNA ladders, respectively. Unlike many commercially available ladders, the 100 bp ladder works well... $20-30 Cost Comparison: While convenient, commercial ladders cost between $200-$500 for 1000 lanes...worth of the 100 bp or the 1 kb ladder. Using a commercial plasmid prep kit increases the costs to $20-30... per ladder: still significantly cheaper than commercial ladders. Are you interested in making your own...

  28. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...your work, downloads, commercialization of patents and letters of recommendation. EB-1A extraordinary ... EB-1B cases, Casseus Law recommends 6-8 letters.  For these recommendations, Casseus Law suggests a mix... pressure off having to find the perfect job immediately after you complete your Ph.D. or postdoc. Foreign.... High Salary –not relevant to most postdocs. Commercial successes in the performing arts- not relevant...an expert is someone who has cited your work. Immediate steps that you can take to prepare for the green...application process. To answer specific questions, we recommend that you reach out to experienced legal counsel...

  29. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    Published
    May 25, 2021, 2:29 p.m.
    ...James Trimmer of the University of California Davis School of Medicine on this grant. Dr. Trimmer has created... ready-to-use-proteins to facilitate use. Dr. Trimmer’s lab will ensure the quality of the antibodies ...Addgene to bring this resource to life,” says Dr. Trimmer. But in order to create such a resource, we need...Initiative has been funding laboratories, such as Dr. Trimmer’s lab, to create recombinant antibodies for use ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

  30. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...code remains in tact! Take notes Comment in your scripts: Comments on what a given line of code does ...the code. # This is a comment in python, R, perl, and ruby // This is a comment in C++ and Java Command...invaluable. In the top directory of every experiment, immediately write a text document with a brief description...and error, and by asking for tips from others. Comment below to add your tips! Many thanks to our guest...

  31. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... and Morrison is nearly identical to that of commercially available kits but instead of binding DNA to...yield and quality similar to that obtained with commercial kits, but in less time. The authors estimate ...completed in 20 to 30 minutes. Additionally, while commercial plasmid prep kits come in discreet sizes (mini...cheaper, but also faster and more flexible than commercial kits.  DNA gel extraction with glass milk Starting...where DNA or RNA can be amplified. Compared to a commercial magnetic bead-based kit, this cellulose-based... mentioned in today’s post? Let us know in the comments! Additional resources on the Addgene blog Read...

  32. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ... you might need to do. In most cases, I would recommend the second option for the experiment above, but...cells later. Eating with friends has a somewhat immediate deadline - even the best of friends get hangry... everything in a Word or google doc that I can comment and make notes on as I progress. I’ve also recently...are far too distracting. (As a side note, I’d recommend the book, “Bored and Brilliant” for learning more...Harvard Business Review article on procrastination recommends testing out how much time you can possibly handle...suggestions, please be sure to note them in the comments! Additional Resources on the Addgene Blog Management...

  33. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...uploaded to Addgene. We also recommend inserting self-cleaving hammerhead ribozymes directly upstream ...construct your own two-input or three-input GRSMs, we recommend using overlap extension PCR and Gibson assembly...reliability of the parts used to build them. We recommend using strong, directional terminators and promoters...transcribed gene and its 5’ UTR. Various self-cleaving hammerhead ribozyme parts are described in Nielsen et al...implement the same gene regulation program. We recommend testing multiple register designs for the same...

  34. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...share both their successes (recommended reagents) and failures (not recommended reagents) along with information... conditions, and more. Users can also recommend or not recommend a particular reagent in the given conditions...documentation that agrees or disagrees with the recommendation. The goal is to easily share and find what ...

  35. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...brain barrier we would currently recommend PHP.V1 or AAV-BR1. We recommend you keep an eye out for emerging... to avoid "leaking" into the brain, would you recommend using the PHP.S capsid over the PHP.eB (the capsid...capsid we have been using)?Would you recommend any liver specific capsid, particularly one that can target...in the blood-brain barrier?   A: For liver, we recommend using one of the natural serotypes such as AAV9...

  36. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...simultaneously allowing scientists to actively comment, annotate, and edit protocols. After my introduction...them public for others to use, and add your own comments. Currently you can find protocols from NEB, OpenWetWare... content. Here at Addgene we’ve been known to recommend protocols listed on OWW, including those for preparing...protocols to share with others. The site allows you to comment on protocols and create lists of your favorite ...software app that I missed, let me know in the comments below and leave your thoughts on how the app works...

  37. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...protein expression and some of the most popular commercial strains designed to avoid them. Why do I need...gal dcm rne131 (DE3) General expression; not recommended for toxic proteins BL21-A1 Tetracycline Arabinose-inducible...expression plasmid from these strains is not recommended as these auxillary plasmids may be isolated during...interest from a T7 promoter on the plasmid. Many commercial strains carry the DE3 lysogen, as indicated by...favorite protein expression tips and tricks! Leave a comment below or browse Addgene’s collection of empty backbones...

  38. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...institutional arguments were not as convincing as the recommendations for postdocs to be on higher salaries (which...Dame sets no minimum, stating that while they recommend NRSA levels, postdocs must be at least at the ... possible outcome: that many postdocs will immediately lose jobs; that tenured professors/mid-career...system sustainable. For years, there have been recommendations that postdoc salaries be raised to $50,000 ...There was ample opportunity to accept these recommendations, and make gradual adjustments towards this ...

  39. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...questions and comments go up early in the day, and the moderators were on top of any comments that were at...Anything”) on r/Science. You can see some of Joanne’s comments on the AMA process below, but we also wanted to... scientists who will find it useful, I highly recommend doing an AMA! Before I turn it over to Joanne,...resources. I’ve said this before, but I also highly recommend taking every opportunity you get to speak publically...

  40. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...Pre and Post (Post recommended) UV Light 0.1 GelGreen Pre and Post (Post recommended) UV or Green (~500nm...important to digest plenty of starting material. We recommend 1.5-2μg of insert and 1μg of plasmid backbone....space to cut out the bands. Because of this we recommend that you use a wide gel comb, run the gel on the...covalent bonds are indicated in red).     We recommend around 100ng of total DNA in a standard ligation...

  41. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking to delve deeper or ...there are plenty of online resources available. I recommend a few: -The recently established Ebola Genome ...EBOV’s past, present, and future, don’t miss Carl Zimmer’s coverage on the subject in the New York Times ...resource to add, please tell us about it in the comments. ...

  42. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...workbenches such as Benchling (commercial, online), DESKGEN (commercial, online), or ProtospacerWB (academic...to other more suitable software such as ApE or commercial workbenches such as MacVector or Benchling. ...specific organisms. For this reason I generally recommend that laboratories that have already attempted ...first time, it is best to choose a tool based on immediate needs and re-evaluate after several experiments...

  43. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...microscopy techniques can be replicated with commercially available consumer-grade components. In this...account in the development process. Widely used, commercially available tools such as the Raspberry Pi, the...become an established alternative to high-priced commercial devices.     Fig. 2: Workflow for open...and assembly designs based around the use of commercially available tools and components. Rapid prototyping...

  44. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...However it’s difficult, especially as a newbie reprogrammer, to decide which method to use. This post provides...suited for these types of studies. What cells are reprogrammed will also affect the outcome, since not all ...research goals. Are you a new or experienced reprogrammer? Which methods do you have experience using?...Let us know about your iPSCs experiences in the comments below! References  1. Sano, Masayuki, Minoru ...PMID: 27764162. PubMed Central PMCID: 5072705. 2. Sommer, Cesar A., and Gustavo Mostoslavsky. "The evolving...

  45. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...repeats separated by an 8-bp long asymmetric core spacer sequence. The asymmetry in the core sequence gives ...site have been created, which contain a unique asymmetric spacer "NNNTANNN", where "N" indicates which ... cre-dependent techniques?  Let us know in the comments! References 1. Bacteriophage P1 site-specific...

  46. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...current lead dTAG molecule is dTAG-13 due its immediate action, potent ligand performance, great depth... and in vivo utility. dTAG molecules will be commercially available in the near future, and we will provide...degradation in your context. Immunoblotting is recommended, while quantitative proteomics experiments will...  References Nabet et al. The dTAG system for immediate and target-specific protein degradation.. Nature...

  47. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...changing flower project was inspired by a Facebook comment.  In our day job, we worked on plants engineered... to see that same anger. Instead, social media comments were more often along the lines of “I hate GMOs...thought we could be taught. They invested in us this summer – $30,000 for research and another $30,000 in mentorship...entrepreneurship, share your thoughts with us in the comments below. We’re happy to answer questions about this...

  48. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...into the AdV by recombination. It is therefore recommended to test for the presence of RCA in the viral ...cells . Alternatively, antibody-based assays are commercially available for faster detection of RCA. 2nd Gen... can be stored at -80C for later use or used immediately for amplification.*Pro-Tip*: The cells will become... concentration kits that do not use CsCl are commercially available. Titer calculation (1 or 10 days) ...

  49. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...sequence/gene - we set our threshold at >100 hits - immediately raises a red flag. For example, AAV-68544 contained... in a given viral prep. With this in mind, we recommend that users titrate their AAV vectors to find the...2015). This higher level of quality control is recommended but not yet required for clinical-grade materials... AAV vectors for your research. Please use the comments section below to let us know if you have any specific...

  50. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...to maximize the alignment length. While this recommendation is not infallible, we have found it saves analysis...sequencing result and can easily translate it, we recommend using blastp over blastx. The primary advantage...confirm a tag or fusion protein is in-frame, we recommend using blastx with the "Align two or more sequences... to confirm your plasmid sequencing results or comments on our suggestions? Share your thoughts here to...
Showing: 1 - 50 of 481 results