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Learn about how CRISPR systems are classified. Addgene's guide to typing CRISPR.

March 18, 2025, 1:15 p.m.

A review of K. phaffii as a model organism and its relevant applications for biotech to basic research.

April 4, 2023, 1 p.m.

Learn about how Cascade-Cas3 can be used as a CRISPR tool to degrade large fragments of DNA.

Feb. 11, 2025, 2:15 p.m.

Data-driven design of Golden Gate Assembly overhangs greatly increases the number of fragments that can be used in cloning.

Aug. 1, 2023, 1:15 p.m.

This post discusses the core replication and resistance features of yeast vectors and explains how these plasmids differ from their bacterial counterparts.

Feb. 25, 2014, 7:11 p.m.

This post discusses the applications, advantages, and caveats to using hybrid guide RNAs, in which DNA bases are substituted for RNA bases.

Jan. 27, 2026, 2:14 p.m.

This post describes evoCAST, a new CRISPR-associated transposase tools optimized for genetic editing in human cells.

June 10, 2025, 1:15 p.m.

Curious about how, exactly, Cas9 does what it does? Dive into the structural biology of Cas9 for an answer!

June 4, 2024, 1:15 p.m.

Comparing and contrasting Cas9 to the other genome editing-capable Cas family proteins.

Dec. 8, 2022, 2:15 p.m.

Learn how you can use plasmids to teach students about functional genomics with your own "Pathways Over Time" project.

May 22, 2018, 1:38 p.m.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Aug. 20, 2020, 2:30 p.m.

Adeno-associated virus (AAV) vector serotypes can help you target specific cell types through their tissue tropism.

Jan. 16, 2025, 2:15 p.m.

We describe the different modular cloning kits available for different applications and organisms to help you find the kit that's right for your experiment.

May 14, 2024, 1:15 p.m.

Baby KJ was treated for his devastating genetic disorder with CRISPR base editing. This post describes the prior advances that made his cure possible.

Sept. 16, 2025, 1:15 p.m.

Cpf1, a newly discovered Cas9 homolog, is smaller than Cas9, can target different sequences, and may more efficiently generate targeted mutations.

Oct. 14, 2015, 2:30 p.m.

Yeast genome modification plasmids have been developed for tagging yeast genes with fluorescent proteins, epitope tags, and more. Various systems are described to help you choose which to use for your research.

Nov. 19, 2013, 2:37 p.m.

Learn how site specific recombinases like Cre-lox and FLP-FRT can be combined for complex control over gene expression in neuroscience experiments and more.

Oct. 19, 2017, 1:54 p.m.

Learn about the process to select the optimal serotype, promoter, and titer for your AAV experiment.

June 1, 2023, 1:15 p.m.

Curious about systemic capsids? Looking for one to test in your experimental system? This 101 is an introduction to systemic capsids uses and types!

Oct. 12, 2023, 1:15 p.m.

Ready to let your tags do the work for you? Learn about self-labelling protein tags and how they can give you flexibility in your experimental approach!

April 16, 2024, 1:15 p.m.