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We narrowed to 138 results for: LOB;

Showing: 1 - 20 of 138 results
  1. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...improving professional development and training in a globalized world where individuals can exchange ideas, network... of that, in the midst of a rapidly changing, globalized environment re-emerging from international financial... mentorship programs like IMP build bridges and global networks. They catalyze the development of knowledge...integrative and eco-social health strategy, and global health equity. She is also Co-founder/director ...
  2. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...both DNA strands via the NUC lobe, as in Figure 6. NUC lobe, green; REC lobe, light blue; guide RNA, dark... has two major lobes, called NUC (for nuclease) and REC (for recognition). The NUC lobe contains the DNA... primarily by the REC lobe. An arginine-rich bridge helix connects the two lobes and helps recognize the...Summary of Cas9 domains, the lobe each domain belongs to, and their roles. Lobe Domain Role NUC... yellow; REC lobe, light blue; bridge helix, purple; sgRNA, dark blue.   The REC lobe binds tightly...the REC lobe, stabilizing the closed conformation, as in Figure 3. Upon DNA binding, the NUC lobe recognizes...Figure 1: A cartoon depiction of Cas9’s two major lobes, REC and NUC, and their subdomains. NUC includes...
  3. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    Published
    May 7, 2020, 1:15 p.m.
    ...to the patients. For example, defects in hemoglobin (𝛽-globin), which cause sickle cell disease and 𝛽...region of gamma hemoglobin (HGB1/2) leads to continuous expression of fetal hemoglobin even in adult patients...edited the fetal hemoglobin promoter and caused persistent expression of fetal hemoglobin to a greater level...can be mitigated with the expression of fetal hemoglobin. Previous studies have shown that one base change...
  4. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...CRISPRi in Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in Caulobacter cells for ... repression in yeast Base editing CRISPRi in Caulobacter crescentus Multiplexed gRNA for human pluripotent...dCas9. Image adapted from Guzzo et al., 2020. Caulobacter crescentus is a popular model organism for studying...system to downregulate expression of specific Caulobacter genes using a catalytically dead Cas9 (dCas9)...Streptococcus pyogenes system does not work well in Caulobacter). To test their CRISPRi system, the Laub lab ...
  5. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    Published
    April 5, 2016, 2:30 p.m.
    ... find themselves on the front lines of emerging global challenges. However, these same researchers face...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution...this map and increase plasmid sharing across the globe! We hope that these awards will both accelerate ...developing countries. As the world continues to face global challenges, we hope to empower those that need ...Conferences Resources on Addgene.org Read about Global Plasmid Distribution Check out the List of Institutions...
  6. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...response, the World Health Organization has declared a global health emergency and called for the fast-tracked...emerging outbreak, as many as 2.2 billion people globally live in areas conducive to the spread of the virus...combined biomolecular tools can distinguish between global strains of the Zika virus (Asian-, African- and...that we can get the diagnostics into the hands of global health responders as soon as possible. Our longer-term...PMC4243060. 4. Messina, Jane P., et al. "Mapping global environmental suitability for Zika virus." Elife...
  7. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...Immunoprecipitation (IP) uses immobilized antibodies, or immunoglobulins, to isolate a specific protein out of a complex...proteins bind only to the Fc portion of the immunoglobulin, leaving the antigen binding sites free to ...Protein A and Protein G have an affinity for immunoglobulins, the strength of the interaction varies between...will bind both the primary antibody and serum immunoglobulins indiscriminately, causing competition for binding...its use as an immunosorbent for isolation of immunoglobulins. FEBS Letters 28:73–76 Additional resources...
  8. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...used mammalian terminators (SV40, hGH, BGH, and rbGlob) include the sequence motif AAUAAA which promotes...termination. Out of those listed, the SV40 late polyA and rbGlob polyA are thought to be more efficient in terminating...of AAUAAA are required for efficient rabbit beta-globin mRNA 3' end formation. Cell. 1987. PMID: 3568131...transcription Polyadenylation: General overview BGH rbGlob SV40 Resources on the Addgene Blog Learn how ...
  9. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...increase accessibility to researchers around the globe. Addgene’s educational resources As Addgene built...designed and easy-to-reference eBooks. Figure 3: Global readership of the Addgene blog in 2023. Views from...videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel, 2023...available and accessible to scientists across the globe?” By asking this question, and by listening to the...
  10. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...Neisseria meningitidis (Nm or Nme) NNNNGATT Campylobacter jejuni (Cj) NNNNRYAC Streptococcus thermophilus...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...).  At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is even smaller than SaCas9 ...editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038...
  11. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...than APC. While APC's global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum ...however, excites at 561 nm, which is close to the global maximum of PE's excitation spectrum where the excitation...maxima, one local maximum at just under 500 nm and a global maximum at around 560 nm, leading to different ...
  12. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...Creatine Deficiency Syndromes (CCDS) that present as global developmental delays or autism spectrum disorder...transporter SLC6A8 impede this transport and result in global creatine deficiency, which is characterized by ...estimated to affect 0.27 to 4.6 per 10,000 people globally. Pustules form from the uncontrolled activation...
  13. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...involved turning on fetal hemoglobin to compensate for defects in the adult hemoglobin produced (Zarghamian... beta thalassemia (TBT), both of which affect hemoglobin. Most of the targeted treatment strategies for...
  14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...lab plasmid requests from Addgene, as a proxy for global activity in our particular scientific subfields...smoothed with a 7-day rolling mean. While this global approximation already shows us a rough "COVID-19...modeling our data, we first chose to re-examine our global data at a finer temporal granularity. For each ...
  15. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...braziliense, Azotobacter spp., Bordetella spp., Caulobacter spp., Enterobacteriaceae, Gluconacetobacter xylinus...Azotobacter vinelandii, Brevibacterium methylicum, Caulobacter crescentus, Desulfovibrio vulgaris, Erwinia carotovora...bacilliformis, Bordetella spp., Brucella spp., Caulobacter crescentus, Escherichia coli, Gluconacetobacter...
  16. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ... coding for heme oxygenase should be supplied (Baloban et al., 2017).  It is important to consider effective...Cells. Cell Chem Biol 24, 758-766 e753 (2017). Baloban, M. et al. Designing brighter near-infrared fluorescent...Science 8, 4546-4557 (2017). Shcherbakova, D.M., Baloban, M. & Verkhusha, V.V. Near-infrared fluorescent...
  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    .../ARE). The Luc-I intron is a chimeric β-globin/immunoglobulin intron that has been commonly used in constitutive...nuclease domain. Feng Zhang's group utilized the bi-lobed architecture of Cas9 to engineer a series of "split...filter out any compounds that are affecting other global cell processes, like translation. This luciferase...hours) in order to avoid complicating effects from global toxicity due to loss of splicing. To this end, ...
Showing: 1 - 20 of 138 results