We narrowed to 35 results for: gc

..., the researchers looked at intrinsic properties (GC content or rare codon percentage) and comparative... and optimizing their algorithm, they found that  GC content and rare codon percentage were not good predictors... predictors. There is not much variation in GC content between many organisms and DNA synthesis companies...companies often work within a narrow range for GC content. Rare codons also are not good predictors because...

...expect titers of 1 × 1013 GC/mL for AAV1, AAV5, AAV8, and AAVrg and 7 × 1012 GC/mL for AAV2. There are no...

...sequence that is over 80% GC. Some IRES sequences also contain regions that are GC rich. Since NGS assembles...particularly difficult to sequence and assemble, including GC rich regions. For example, the CAG promoter, a hybrid...

...genome copies (GC) per reaction. Since AAV titers tend to be in the range of 1012 to 1013 GC/mL, you must...concentration can then be used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = ...

...secondary structure, palindromic nature, and high GC content. This instability can cause partial loss ...through ITRs due to their secondary structure and high GC content, but some companies now offer Sanger sequencing...

...early-onset GC and one with a mutation at R273H to match a mutation frequently found in late-onset GC. HiBiT-TP53...identified that in a set of patients with gastric cancer (GC), TP53 was one of the most frequently mutated genes...

... AAV applications.  Tips & FAQs Due to their high GC content and hairpin structures, ITRs are prone to...might fail to correctly read through ITR sequences. GC tails are often located immediately outside of the...

... 8.5 x 10e10 GC/mL. The lot of viruses used (100798-AAV, lot #v15287, titer 1.7x10e13 GC/mL and 100799...100799-AAV1, lot # v14715, titer 2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively...

...match the size of your template. Amplification of GC-rich plasmids is facilitated by the addition of DMSO...functionally important regions span several kb (or are GC-rich and therefore difficult to sequence), it may...

...are difficult to sequence by NGS. These can include GC-rich and repetitive regions. These regions require...

...you can provide location, titer dose (Example: 1E13 GC/mL) and subject age at time of injection. How long...

... target. NgAgo also appears to outperform Cas9 in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated...

...higher DNA binding affinity for AT-rich regions vs. GC-rich regions from 40 mM to 100 mM NaCl but stained...

...codon for C-terminus tagging) GC content of the crRNA (aim for 40-60% GC content) crRNA activity Local...

...bp with the template and if the sequence has high GC or AT content, increase the overlapping region of...

...assemble using the Illumina MiSeq platform, such as GC-rich regions and highly repetitive sequences and/...

... termination, and it relies on the formation of a GC-rich hairpin in the RNA transcript followed by a ...

...interest. SpCas9’s 3’ NGG PAM occurs frequently in GC-rich genomes, but a PAM is not always available near...

...Cas9. Third, it can be used to target “difficult” GC-rich regions of the genome, which seem resistant ...

...from that of Cas9 (3’-NGG) in both distribution and GC-content, which might improve editing in AT-rich genomes...