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Showing: 1 - 20 of 171 results
  1. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...consider and quantify the impact of scientific publications. Ventures like Impact Story seek to find ways...holiday season want the work you are doing to have an impact, to move science forward, or perhaps even society...and has been to publish in journals with a high “impact factor,” a measure that dates back to 1975 and ... of other ways to define and measure scientific impact. Even if we stick to citation counts for a moment...October entitled “Rise of the Rest: The Growing Impact of Non-Elite Journals” found that the proportion...half.   The effect of online access on scientific impact Partly, the story has to do with online accessibility...journal publications really the best way to measure impact? As that Nature report also noted, papers detailing...
  2. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ... across regions, with Europe most impacted and China least impacted. The inferred weekend effect is, in...date range is, they could be the most impacted or least impacted. These results highlight the instability... last six months, COVID-19 has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has...unique PIs/labs placing orders per day) on dates impacted by each effect. The inferred parameters suggest...methods for fundamental scientific discovery and high-impact applications.       Alvin Hsu is a graduate student...
  3. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...the lab could make a big difference in our global impact as scientists. Many scientists are already leading...toward responsibility in our lab’s environmental impact, but we can all do more to make research more environmentally...and companies have the potential to make a huge impact directly on waste, but also on culture. Encourage...your workplace and you could end up making a huge impact on how waste is handled. A lot of places have small...
  4. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...architecture of my cells,” or “How does this mutation impact the types of cells found in my tissue.” It is based...antibody performs differently and some antibodies impact the performance of other antibodies. But, once ...the right protocol, multiplex IF can give you impactful and beautiful results. Performing an immunofluorescence...fixation maintains sample morphology with minimal impact to the target epitope. There are multiple methods...exclusive to IF, but they can have a significant impact on the outcome of your IF experiment. Many of the...choose for your experiment will have a significant impact on the protocol you use and the ultimate outcome...
  5. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...environmental impact on a scale of 1-10: the lower the score, the lower the environmental impact in terms ... product? How can we consider the environmental impact of those processes? Who even regulates them? You...
  6. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...response initiatives. Accelerate the translational impact of research Universities have also been stepping...research synergies and accelerating the translational impact of research activities. By streamlining the development...by coming together, we can create a significant impact on both clinical care and public health for this...
  7. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...this time more visible and thereby have a positive impact on science. ReFigure saves time, knowledge and ...definitely give you credit for it and increase your impact! Download ReFigure extension and visit ReFigure.org... with Joe Akin. She envisions a world where the impact of teaching and doing science is rapid, concrete...
  8. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...the focus shifts to choosing an unexplored and impactful research topic that aligns with your interests...when selecting a research project. What will the impact be in a best case scenario? If you get your dream...what will your paper look like? What will be the impact on your field? You want to choose a research question...
  9. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...are crucial in demonstrating the true innovation, impact and, in turn, the big picture of the project. For... For example, how can future regulations impact your work? What design variables need consideration? Enrich...project is an innovative solution with a strong impact, or a journalist does not understand the high-level...
  10. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...realizing it. Yet, this selection is crucial as it will impact downstream outcomes of an experiment, including...Grimm & Lavis, 2022). These variations directly impact the way you can utilize these dyes. The most-used...emission of labeled agonists and antagonists, impacting the interpretation of experimental results. It's...dopamine D2/D3 receptors (Allikalt et al., 2020) Impact of labeling process: The process of dye labeling... on the respective fluorochrome. Brightness is impacted by both the excitation coefficient, characterizing...multiple dyes during analysis. Image resolution is impacted by several factors. Firstly, brighter dyes enable...
  11. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...lost during fixation with these chemicals, which impacts sample structure and could also cause your target...destroy it for others. That said, organic solvents’ impact on lipids means that they are able to permeabilize....   Figure 1: Choice of fixation method impacts antibody binding. Formaldehyde cross-links the ...detergent has different chemical properties that will impact the level of permeabilization and membrane integrity...
  12. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...week to celebrate the scientists who make a big impact in open science. Depositors consistently contribute...depositor fun facts for a quick way to grasp the big impacts depositors have on science globally. *All data ...
  13. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...investigators realize too late that they have missed the impact of dark, non-fluorescent, and misfolded FP-fusions...quantitative imaging experiments or even negatively impact cells (see Figure 1). When behaving properly, ... the function of resident ER chaperones and may impact cellular function or possibly viability. FP misfolding.... It remains unclear whether the FP misfolding impacts fusion protein function and this could lead to ...
  14. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...Discrepancies in transduction efficiency can have a major impact on experimental design and interpretation of results...transduced by the vector. One method for minimizing the impact of this phenomenon is to use a retrograde recombinase-expressing...cell bodies of the projection neurons reside.   Impact for scientists While the mechanisms by which retrograde...
  15. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ... resulting in variable gene expression that can impact observed phenotypes. Factors such as the growth...medium, growth temperature, and growth rate can all impact plasmid copy number in a cell. One can attempt... from a plasmid to the genome without greatly impacting the level of gene expression. For example, the...
  16. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...experiment. Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome...chromosomes within a day. Another factor expected to impact repair is that the Cas9 protein doesn’t immediately...in determining how the structure of broken ends impacts how they are repaired. He works in Dale’s lab.  ...
  17. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...long-term science outreach, may have the most profound impact (15). Diversity and inclusion in science, technology... immigrants, and minorities who have had great impacts on STEM (21–26). Increased scientific funding...communities (something already encouraged by “Broader Impacts” requirements for certain grants) (28). As one ...Science Foundation 15-008: Perspectives on Broader Impacts. The Pipettepen: The March for Science Raises Concerns...
  18. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...plasmid. The requirement for additional DNA can impact the transfection efficiency of the packaging cell... and the timing and number of harvests will all impact the titer. Importantly, optimized conditions for...virus, volume and timing of transduction will all impact the recorded titer and should be taken into consideration...
  19. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ... over-reliance on the perfect story for a “high impact” publication can lead to the publication of rare... data even if it isn’t considered immediately impactful. Down the line we may see further adoption of ...criticisms that focus less on perceived novelty and impact. Journals could also begin requiring reproducibility...health of the scientific enterprise and not just impact. Cause #3: Poor experimental design It’s easy to...
  20. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...are sharing stories of our repository’s positive impact on science and scientists around the globe. Our...CRISPR plasmids to labs in ~100 countries.  The impact Addgene’s CRISPR collection contained 197 plasmids...citations.  CRISPR is a story that centers around the impact of science, but our data makes it clear that it...
Showing: 1 - 20 of 171 results