...channels and pumps in the membrane. An emerging interdisciplinary field of biology known as bioelectricity is...regenerating tissues without using genome engineering. Planarian worms cut into three segments “remember” which...protein-mediated cues control polarity during planarian regeneration." Developmental biology 339.1 (2010...

...are delivered by feeding them to planarians. Depending on which planarian species one is working with, the...accessible or too challenging for amateur scientists. Planarians, wormy creatures commonly found in freshwater...provide a good potential solution to this problem. Planarians are easy to buy, cultivate, and have interesting...advanced developmental biology experiments in planarians with their recent plasmid deposit. These plasmids...in four inexpensive and commercially available planarians: Girardia sp., Dugesia dorotocephala, Phagocata...into plasmids to investigate for each of these planarian species. These plasmids all have a PR-T4 vector...technique. The expression patterns of 11 different planarian genes (all of which are available through the ...

...Indels by Decomposition (TIDE) (Brinkman et al., NAR). This technique uses the trace file generated in...repair events and small edits (Brinkman, et al., NAR). It works just like TIDE (outlined above), except...42, Issue 22, Page e168. https://doi.org/10.1093/nar/gku936 Eva K. Brinkman, Arne N. Kousholt, Tim Harmsen... 46, Issue 10, Page e58. https://doi.org/10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri N, Giugno...outlined below, the technique can be used as a preliminary validation on your bulk population. This way ...prediction analyses like CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing...

... Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124 Martin AS, Salamango DJ, Serebrenik AA, Shaban...Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332 Standage-Beier K, Tekel SJ, Brookhouser N,... Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713 Additional resources on the Addgene blog ...

...Acids Research 28:292–292 . https://doi.org/10.1093/nar/28.1.292 Sauna ZE, Kimchi-Sarfaty C (2011) Understanding...Acids Res 15:1281–1295 . https://doi.org/10.1093/nar/15.3.1281 Slimko EM, Lester HA (2003) Codon optimization...

...Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing: precision... Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555 Resources at the Addgene Blog Listen to our...wrong place, but because the target window is more-narrow, there are fewer gRNA to choose from, and thus ...

...Acids Research 47:e23–e23. https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM, Thakore... Research 44:5615–5628 . https://doi.org/10.1093/nar/gkw159 Williams RM, Senanayake U, Artibani M, Taylor...

...Nucleic Acids Res. 2007;35(5):1402-10. doi:10.1093/nar/gkl1108. Epub 2007 Feb 6. PubMed.  ...

...Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (2013) Efficient... Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert JK, Higgins...

...Research, 42(10), 6091–6105. https://doi.org/10.1093/nar/gku241 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto...Research, 48(4), 2000–2012. https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg... J., . . . Koonin, E. V. (2015b). An updated evolutionary classification of CRISPR–Cas systems. Nature... Yakunin, A. F., . . . Koonin, E. V. (2019). Evolutionary classification of CRISPR–Cas systems: a burst...

... Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Citorik RJ, Mimee M, Lu TK (2014) Sequence-specific...Acids Res 44:e143–e143 . https://doi.org/10.1093/nar/gkw625 Resources on the Addgene Blog Check out our...organism/gene interactions with the host. This scenario allows for several potential CRISPR applications...microbiomes titling it the Bee microbiome toolkit (Leonard et al., 2018).  Base Editing Among the newest approaches... 31:233–239 . https://doi.org/10.1038/nbt.2508 Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, ...

...Acids Res. 2021 Sep 7;49(15):8785-8795. doi: 10.1093/nar/gkab507   Resources on Addgene.org Addgene’s CRISPR...

... Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle...

...Nucleic Acids Research. 2014;43(3):e17. doi:10.1093/nar/gku1198...

...Research, 46(12), 6140–6151. https://doi.org/10.1093/nar/gky457 Park, K., Han, E., Paulsson, J., & Chattoraj...Rozgaja, T. A., Gupta, R., Dyson, K., Rao, P., & Leonard, A. C. (2018). Origin recognition is the predominant...

... Research 30:3870–3879 . https://doi.org/10.1093/nar/gkf495 Watson JD, Crick FHC (2003) Reprint: Molecular...negative. But these negative depictions serve as cautionary tales for the entire scientific community and...

...Nucleic Acids Research 44: . https://doi.org/10.1093/nar/gkw930 Yu Y, Wu X, Guan N, Shao J, Li H, Chen Y, ...

...October 2000, Pages 3752–3761, https://doi.org/10.1093/nar/28.19.3752Wong M; Medrano J. Real-time PCR for mRNA...

...deposited by the Gradinaru lab. For simplicity, in each section we’ll list the Gradinaru capsids first, ... X., Luo, Y., Einarsson, P. H., Greenbaum, A., Jang, M. J., Deverman, B. E., & Gradinaru, V. (2020). Multiplexed...capsids in our repository are deposited by the Gradinaru lab. You can visit our Caltech Systemic Capsids...not otherwise attributed were deposited by the Gradinaru lab.  AAV-PHP.B was the first systemic capsid ...capsid developed and deposited by the Gradinaru lab (Deverman et al., 2016). Compared to AAV9, it effectively...specificity than AAV-PHP.eB. For this reason, the Gradinaru lab anticipates that high-dose AAV-PHP.AX will...

...factor and promoter pairs referred to as binary systems. Binary systems are genetic tools that consist ...repressible element to this binary system (making this a repressible binary system). The GAL80 repressor...default ON to an OFF state, as well as repressible binary systems. In recent years, a number of more complex...consumption phase of yeast culture. Repressible Binary Systems GAL4/UAS In Drosophila or development studies... gene expression across development. One common binary system is the GAL4/UAS system isolated from yeast... Valle Rodriguez et al.   LexA/lexAop A second binary system is LexA/lexAop, developed by Lai and Lee...QS and quinic acid. From Riabinia et. al. Using Binary Systems Together You may be wondering, “why is ...