We narrowed to 855 results for: NTS

...work with your colleagues, supervisors, clients or even students, your ELN should allow you to collaborate...paper-based record keeping barely copes with the growing amounts of digital data generated in labs. While many labs...different ELN features, ranging from whether respondents saw them as not important to very important. ...explored the barriers to adoption within academic environments to better understand the issues labs are facing...limited budgets, “a large percentage of survey respondents indicated that cost was a significant barrier...system within their institution. In the study, respondents were also asked about how important ease of use...from paper to digital for example): “… 99% of respondents indicated that ease of use would influence their...

... your protocols, designed your experiments, and prepared your reagents. You’re going to engineer the best...assemble up to 6 different fragments in a prescribed order. These fragments can be pooled in one tube ...PCR to generate DNA fragments with 15-40 bp of homology at their ends. These fragments are then combined...methods are more amenable to combining multiple DNA fragments than others. In today’s post, we will focus on...in an hour or less. The ligations combine the fragments in an order determined by their homology. You ...cloning process. Time spent actually doing the experiments are also generally comparable so the best way...optimize reagent concentration, or purify DNA fragments from gels. If you’re relatively new to the cloning...

...problem.  Pre-block with Fab fragments (Figure 2C) Speaking of Fab fragments… Another strategy for species-on-species...been bound up by the Fab fragments. Note that you should plan to use Fab fragments that are from the same...should not recognize Fab fragments from the same species and 2) these fragments will be more likely to ...include no primary antibody controls in your experiments. If you do find high levels of background staining...that is directly conjugated to your reporter circumvents the issue. However, directly conjugated primary...pre-treating the tissue with unconjugated Fab fragments that recognize the endogenous IgGs (Lu and Partridge...Partridge, 1998). After washing away unbound fragments, you can then apply your primary and secondary antibodies...

...Complex experiments Finally, additional controls may be required for more complex experiments. A multiplexed...helpful to focus on something controllable, like experiments (and their controls!). This blog post will discuss... the ins and outs of controls for biological experiments, starting with general controls and then moving...design thoughtfully and thoroughly controlled experiments.  Broadly useful controls Positive and negative...negative controls Most biological experiments require positive and negative controls to ensure proper interpretation...group.     Figure 1: A, Most biological experiments will benefit from positive (+) and negative (...group and C, technical replicates or repeated measurements will increase the power of the results. In this...

... antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press,...candidate at Northeastern University. Working with plants doesn’t always have to be a time-consuming process...reproducible. Prior to creating transgenic cultures or plants, our transient expression method enables us to ...leaf transformations The development of transgenic plants is a common problem in non-model organisms. Therefore...choice for investigating gene function, as these experiments can be done in days to weeks instead of months...roseus. A common method for overexpressing genes In plants like Arabidopsis and tobacco, Agrobacterium infiltration...more easily quantify the effect of the different treatments on transformation success. Some reporter gene...

...curate reagents from these emerging organisms so scientists can easily find the useful new reagents that...Cnidarian-Dinoflagellate symbionts (Virginia Weis) Sea anemone Aiptasia - algae Breviolum minutum symbionts are important...Westwood) Dodders are parasitic plants that steal all their food from other plants and are unusual enough to...between gene function and phenotypes in diverse plants, animals, microbes, viruses, and fungi for which...for scientists and educational programs for K-12 students. You can see why I was excited to be invited to...at least 6-7 times and on at least 2 separate continents. Mimulus (Andrea Sweigart) Monkeyflowers were...and Asclepias curassavica (Gerog Jander) Milkweed plants are known to many as the only Monarch butterfly...

...many microscopy experiments with fluorescent proteins (FPs) localized to compartments other than the cytoplasm...misfolded FP-fusions on quantitative imaging experiments and cell physiology in general. Pitfalls of Fluorescent...apparent and can confound quantitative imaging experiments or even negatively impact cells (see Figure 1...sequences, which often have absolute position requirements. For example, a KDEL ER retrieval sequence only...that FPs are often poorly suited for cellular compartments other than the cytoplasm. Generally, FPs evolved...in or were engineered for use in cytoplasmic environments. However, approximately 40% of human (and most... localize to chemically distinct subcellular environments, including the organelles that compose the secretory...

...conditions. Purifying DNA fragments Once you’ve generated your DNA fragments, it is always a good habit...digested fragments or PCR products from a gel. You will avoid contamination from other DNA fragments and you...nanodrop to assemble the  fragments based on their molar ratio. Assembly of the fragments There are multiple ... way to do it (i.e. avoid assembling too many fragments at once, if it is possible). There are many softwares...could affect or delay your experimental work. Fragments generation After you’ve designed your cloning ...cloning scheme, you’re now ready to generate fragments. You can generate the parts of DNA that you want to assemble...between the different enzymes. Always check the fragments size of your digestion on an agarose gel. If the...

...neuron subtype) and specific subcellular compartments for measurements. All of the sensors discussed below ... you are measuring the events-of-interest. Sensors suitable for ER measurements include: CEPIA (additional...are used to monitor calcium concentrations and transients in living cells and organisms. Unlike chemical...slower). GCaMPf may be suitable for very rapid measurements, like action potentials in neurons. jGCaMP7 ...calcium concentrations in different subcellular compartments can vary greatly. Additionally, the calcium ... the (1) binding of calcium, (2) allosteric rearrangements of the sensor, and (3) subsequent change in...you combining your calcium reading with other measurements, specifically fluorescent ones? Many of the ...

...uniquely challenging to apply in dicotyledonous plants, or dicots. But Jae-Yean Kim’s lab recently "broke... strategy uses new combinations of PE protein components (Figure 1), an altered epegRNA delivered with...chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited gene to confer resistance..., two weeks after herbicide was applied to both plants. Adapted from Vu et al., 2024 bioRxiv, under CC-BY-NC-ND...both tomatoes and Arabidopsis, suggesting the improvements may be broadly applicable to dicot species. .... In addition, they generated offspring tomato plants that inherited the genomic edit but not the editing...desired edits in tomato and Arabidopsis. Nature Plants, 10(10), 1502–1513. doi: https://doi.org/10.1038...