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We narrowed to 599 results for: REV;

Showing: 91 - 100 of 599 results

  1. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    Published
    July 23, 2020, 1:20 p.m.
    ...In this study, the labs found that anti-CRISPR prevents Cas9 activity in HEK293 by reducing editing from... Acr samples. Acr proteins can also be used to prevent dCas9 tethering to DNA. Since these early anti-... AcrIIC2 interactions with Cas9 interferes and prevents the correct assembly of the crRNA-Cas complex ...binds to the HNH endonuclease domain of Cas9 and prevents target DNA cleavage (Harrington et al., 2017)....vectors. Having a way to shut off CRISPR-Cas systems prevents the off-target effects and cytotoxicity that result...

  2. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...inputs. This study revealed that these neurons received more inputs than was previously thought. The differences...subsequently higher and higher order neurons doesn’t reveal network connections in a useful way, but rather... tracing is a powerful tool that can be used to reveal brain circuitry, and continue to contribute answers..., Berninger B. Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons...

  3. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...explosion of software tools accompanying the CRISPR revolution. (See omictools for even more.) However, none...step of mutating guide sequences in a way that prevents re-cutting but does not disrupt gene expression...CrispyCrunch is how it mutates guide sequences to prevent re-cutting of the sequence inserted by HDR. Although...mutating a single base pair may not be enough to prevent re-binding. Lastly, the sequence inserted by HDR... target regions Design donor RNA Design primers Review and order reagents Perform your wet lab experiment...

  4. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...and I recommend Bustin, 2017 for a more in-depth review.)  Your amplicon should be between 70-200 bp long...ideally located close to either the forward or the reverse primer.  There is a list of useful primer design...efficacy with a standard curve. If relying on previously-generated validation data, be aware that different...transcripts. However, adding too many reagents can prevent your reaction from reaching the plateau stage after...sample compared to your control. But as we said previously, just because it’s used often doesn’t mean it...York 2014Bustin S; Huggett J. qPCR primer design revisited. Biomolecular Detection and Quantification. Volume...

  5. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...thus structural epitopes, but can reveal linear epitopes previously hidden within the protein. Created...cross-linking can block the epitope of some targets, preventing your primary antibody from binding (Fig.1). The...cleaner results by not permeabilizing as you’ll prevent binding to any target protein that is still on ...preserve sample morphology, while organic solvents can reveal otherwise hidden epitopes. However, keep in mind...

  6. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ... unraveling the mysteries of behavior. We’ve previously discussed some of the general considerations ....  What’s involved in locomotor sensitization? Previous research has shown that regions such as the nucleus...transmission leads to locomotion sensitization. (for review see van Huijstee & Mansvelder  2014). Scientists...the locomotion of the animal. This confirms the previously suggested inhibitory mechanism of locomotor sensitization... times are ahead for behavioral neuroscience to reveal insights into the complex relationship between ...

  7. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    Published
    Aug. 13, 2019, 12:58 p.m.
    ...experimental treatment can be monitored. This can reveal previously unknown functions for genes, and lays the ...of the zebrafish genome was published in 2013, revealing that about 70% of human genes have a counterpart... ways. Zebrafish mutants In the 1990’s, two revolutionary large-scale random mutagenesis screens produced...unlocked novel research into genetic compensation, a previously unknown mechanism of gene regulation (El-Brolosy...

  8. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...always to the same extent. As described in our previous Origin of Replication post, DNA replication is...bacteria Before looking at an example plasmid, let's review how E. coli normally replicate their chromosomal...sequence forming this pseudoknot, so its binding prevents the mRNA from folding and blocks RepZ translation...high levels of the lncRNA bind to RepZ mRNA and prevent this pseudoknot from folding, blocking replication...bacterial plasmids. Microbiology and Molecular Biology Reviews: MMBR, 62(2), 434–464. https://doi.org/10.1128/...

  9. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...publications. The policy was not an overnight revelation, rather it was the result of consultations between... rationale includes increasing the ability of reviewers (and, once published, anyone) to detect possible...apply different or novel tools and assumptions to reveal findings that the originator was oblivious to, ...called Scientific Data, which aims to act as a peer-reviewed repository for “scientifically valuable” datasets...or table, clicking on the published chart could reveal the underlying data and allow other analyses “on...

  10. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...gene are amplified. RPA reactions also contain reverse transcriptase to produce cDNA. Isothermal amplification...are designed, each binding to one strand of the reverse-transcribed target sequence that are present close...with 9V battery Approach :This preprint uses a previously developed method to detect SARS-CoV-2 in the ...samples. First, RNA is extracted and subject to reverse transcription. This is then amplified by PCR using...detect fluorescence upon cleavage. Highlights: Reverse-transcribed RNA samples are amplified by PCR instead...
Showing: 91 - 100 of 599 results