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We narrowed to 513 results for: EMB

Showing: 271 - 300 of 513 results

  3. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell types:...oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology...

  4. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...days, weeks, or months. It is always helpful to remember that if a PI asks for assistance in the grant ...of the statements may be slightly far reaching, remember that you are writing a proposal to test hypotheses...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...have a half life of 84 hours in primary mouse embryonic fibroblasts. This fusion protein can be used to... The researchers tested several scenarios from embryo development to myeloid proliferation in acute myeloid...

  7. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...TET, can, like the iron ball-and-chain that they resemble, actually impede your investigation into the regulation...CpG so that nothing else can interact with it. Remember, the goal of this experiment is to expose an unmethylated...

  8. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...sensors available that will localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an ...the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References and Resources...

  9. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...variants:   Figure 2: Inner mitochondrial membranes imaged with structured illumination microscopy...2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https...

  10. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...will be held on Friday 16th and Saturday 17th September respectively. Join us to discuss advocacy efforts...scientists, both young and established, as they embark on or continue their careers in science-related...

  11. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...overlooked regulatory fragments of these transcripts. I remember when small RNA was just something we ran off the...hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they are making fairly...

  12. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...break), write down some notes on the card so you remember who they are and what you’ve talked about. After... Resources on the Addgene website Become of a member of the Addgene depositor community ...

  13. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots to use for qPCR...Tips When calculating the titer for your AAV, remember to account for the dilution of the sample for ...

  14. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... adaptable into future magnetogenetic tools.  Embracing science in the open The York Lab operates with...applications across a wide range of topics. He embraces open science, self-publishing directly on his ...

  15. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...associate with the endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when ...Chem Biol. 2015 Sep;11(9):713-20. doi: 10.1038/nchembio Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi...

  19. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...development of vertebrate embryos.  The first 3D reconstructions of clear embryos were obtained by using ...

  20. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...else you’d like to say to our readers? Patron: Remember plants. Every time you look at your plate, think...Journal. A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner...

  21. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...likely due to quarantining {% video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist..._playlist=True, viral_sharing=False, embed_button=False, autoplay=False, hidden_controls=False, loop=False...

  22. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources on the Addgene blog...

  23. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which...

  24. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...!). In 1997, Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry...and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the recipe...

  25. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...want to know the orientation of the insert. One member of this type of primer pair anneals to a sequence...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about...

  26. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...

  28. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing and non-...

  29. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...Maybe you’ll need to care for an elderly family member, want to train for a triathlon, finally buy that...laundry, take public transportation, call a cab and remember their own school assignments, the better. In my...

  30. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used to modify genomic loci that...SpCas9 (Amrani et al., 2018). When choosing a Cas9, remember to check that your tracrRNA and crRNA (or synthetic...
Showing: 271 - 300 of 513 results