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  1. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay...

  2. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...point, decide early on what to do in a namespace collision. It may even be worth it to have extra columns...solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence, insert, ...

  3. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...consider—we have loaded those we are aware of in our checklist for tissue and cell research. Using cells from...of this literature in our health and medicine checklist. A common worry for researchers wanting to consider...

  4. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...experiments The first thing you will need is to get the list of effective gRNA sequences targeting your genes...pool. (Adapted from Wong et al., 2016). With a list of gRNA targeting sequences, you can then readily...

  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse... transgenics. A few of the most common uses are listed below. Cre-dependent gene expression – placing...

  7. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...for the administrative side of the grant to be established. Take time to read and fully understand the RFA...achieving each aim. A funded proposal typically lists the deliverables, explains 1-2 potential pitfalls...

  8. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...tool that you are looking for and check our curated list of biosensors too, as another laboratory may have...designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in...

  10. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ...fragments may be near the upper limit of what can be accomplished with Golden Gate Assembly—even when getting ...

  11. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). ...

  12. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...but so is where you’re building it! Table 1 has a list of Fragmid destination vectors currently available... you to fill in module fragments from a dropdown list for every module. Both the modules and the components...

  13. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

  14. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...up clear tasks for each member of the team to accomplish so that many elements of the project were able...Addgene Blog Read other Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member...

  15. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org...both polyadenylation and termination. Out of those listed, the SV40 late polyA and rbGlob polyA are thought...

  16. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...vectors? Viral genome sequencing Thanks to the establishment of in-house sequencing capabilities and Seqwell...not map to the reference sequence. The resulting list of hits is then carefully reviewed. As mentioned...

  17. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ... In that case, an RQ value of one (or, more realistically, between 0.80-1.20) would mean you have one ... Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014 Jan 21. doi:10.1002/0471142905.hg0721s80 ...

  18. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ... PCR was invented in the early 1980s by Kary B. Mullis who later shared a Nobel Prize in Chemistry for...PCR is that it can amplify DNA using only a short list of reagents and several heating and cooling steps...

  19. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...The tradeoffs between various cloning methods are listed in Table 2. For example,  chaining-based cloning...used in various chaining-based cloning methods are listed in Table 3. The goal of high throughput cloning...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...not better, than antibodies for all of the above listed molecular techniques, but they can also be expressed...

  21. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...

  22. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...examines the inherent brokenness of scientific publishing and research funding. I’ve been aware of these... place. Can we address problems in scientific publishing without acknowledging the systemic sexism in ...

  23. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...blog posts centered around scientific papers and publishing Find tips for leading a great meeting ...

  24. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ... to reflect upon before they attempt to make essentialist connections between biology and ancestral groups...the long and ugly history of reporting false essentialist links between biology and ancestry group as ...

  25. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...classic, net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...In past decades, further refinements to this established dye structure have yielded commercial panels ...

  26. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ... what institutions are actually doing (using the list of 340 institutions with postdocs in science, engineering... attempted to check 123 of the 340 institutions listed as having postdocs, representing ~85% of the postdoctoral...

  27. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...develop communication strategies, or understand, and publish on the health and economic benefits of novel drugs...Experiment with New Careers while in Grad School Listen to Michael Koeris' Journey from Grad Student to...

  29. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...polling some Addgene colleagues, we’ve come up with a list of things to consider as you make this transition...careers and desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available ...

  30. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...SARS-CoV-2 using a lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK...

  31. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...be time-consuming or even very frequent, but establishing a few minutes every month dedicated to thinking...Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face...

  32. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able...

  33. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...to a job that requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have...experience in [field of research], and have excellent [list the most important skills for the position]. I am...

  34. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...Andreas Rett, who tried to raise awareness and published his observations in a German medical journal. ...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...

  35. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5... using moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are highly...

  36. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...a particular sequence. Browse Addgene's curated list of Bacterial Expression Systems Table 1: E. coli...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

  37. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...most used pairs to measure FRET. The table below lists plasmids that can be used to create your choice ...measurement methods as feasible when first beginning to establish the FRET methodology for a given experiment. You...

  38. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...amassed nearly 500,000 ‘likes’, while their website lists over 200 partner organizations, and 514 satellite... is required. This is by no means an exhaustive list and every marcher is not necessarily marching for...

  39. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity to TO1-biotin...ingredients in the aptamer soup In addition to these established RNA aptamer fluorophores, more are in development...

  40. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...address many areas of microbial research like metabolism. Transport, biofilms, and much more. Because ...Resource, a breeding facility for X. laevis and X. tropicalis, the two most commonly used species of frog. ...
Showing: 361 - 400 of 611 results