We narrowed to 619 results for: LIS

...incorrect; take it instead as valuable feedback.  Listen. Think about the questions you’ve received, read...read the comments, seek feedback from others, and listen quietly as people discuss their experiences. If...

...point, decide early on what to do in a namespace collision. It may even be worth it to have extra columns...solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence, insert, ...

...consider—we have loaded those we are aware of in our checklist for tissue and cell research. Using cells from...of this literature in our health and medicine checklist. A common worry for researchers wanting to consider...

...experiments The first thing you will need is to get the list of effective gRNA sequences targeting your genes...pool. (Adapted from Wong et al., 2016). With a list of gRNA targeting sequences, you can then readily...

... a very big way. In one of his latest papers, published in Molecular Systems Biology in November, Voigt...(2014).   We talked with Voigt about this collision that’s taking place between CRISPR technology ...

... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse... transgenics. A few of the most common uses are listed below. Cre-dependent gene expression – placing...

...for the administrative side of the grant to be established. Take time to read and fully understand the RFA...achieving each aim. A funded proposal typically lists the deliverables, explains 1-2 potential pitfalls...

...at the Faculty of Sciences of NOVA University in Lisbon, Portugal. She leads the Glycoimmunology group ...approved by the FDA in 1939 for the treatment of botulism and Lambert-Eaton myasthenic syndrome (LEMS) because...

...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). ...

...but so is where you’re building it! Table 1 has a list of Fragmid destination vectors currently available... you to fill in module fragments from a dropdown list for every module. Both the modules and the components...

... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ...fragments may be near the upper limit of what can be accomplished with Golden Gate Assembly—even when getting ...

...tool that you are looking for and check our curated list of biosensors too, as another laboratory may have...designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in...

...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

...up clear tasks for each member of the team to accomplish so that many elements of the project were able...Addgene Blog Read other Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member...

...annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org...both polyadenylation and termination. Out of those listed, the SV40 late polyA and rbGlob polyA are thought...

...vectors? Viral genome sequencing Thanks to the establishment of in-house sequencing capabilities and Seqwell...not map to the reference sequence. The resulting list of hits is then carefully reviewed. As mentioned...

... PCR was invented in the early 1980s by Kary B. Mullis who later shared a Nobel Prize in Chemistry for...PCR is that it can amplify DNA using only a short list of reagents and several heating and cooling steps...

... In that case, an RQ value of one (or, more realistically, between 0.80-1.20) would mean you have one ... Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014 Jan 21. doi:10.1002/0471142905.hg0721s80 ...

...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...not better, than antibodies for all of the above listed molecular techniques, but they can also be expressed...

...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...

...examines the inherent brokenness of scientific publishing and research funding. I’ve been aware of these... place. Can we address problems in scientific publishing without acknowledging the systemic sexism in ...

...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...blog posts centered around scientific papers and publishing Find tips for leading a great meeting ...

... to reflect upon before they attempt to make essentialist connections between biology and ancestral groups...the long and ugly history of reporting false essentialist links between biology and ancestry group as ...

...The tradeoffs between various cloning methods are listed in Table 2. For example,  chaining-based cloning...used in various chaining-based cloning methods are listed in Table 3. The goal of high throughput cloning...

...classic, net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...In past decades, further refinements to this established dye structure have yielded commercial panels ...

...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able...

...to a job that requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have...experience in [field of research], and have excellent [list the most important skills for the position]. I am...

...be time-consuming or even very frequent, but establishing a few minutes every month dedicated to thinking...Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face...

...transduction was not detected. They also used an ELISA to measure binding of PHP.B to a soluble version... See Supplementary Table 3 in Huang et al. for a list of PHP.B permissive and non-permissive mouse strains...

...Andreas Rett, who tried to raise awareness and published his observations in a German medical journal. ...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...

...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...SARS-CoV-2 using a lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK...

...polling some Addgene colleagues, we’ve come up with a list of things to consider as you make this transition...careers and desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available ...

... what institutions are actually doing (using the list of 340 institutions with postdocs in science, engineering... attempted to check 123 of the 340 institutions listed as having postdocs, representing ~85% of the postdoctoral...

...develop communication strategies, or understand, and publish on the health and economic benefits of novel drugs...Experiment with New Careers while in Grad School Listen to Michael Koeris' Journey from Grad Student to...

...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5... using moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are highly...

...a particular sequence. Browse Addgene's curated list of Bacterial Expression Systems Table 1: E. coli...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

...most used pairs to measure FRET. The table below lists plasmids that can be used to create your choice ...measurement methods as feasible when first beginning to establish the FRET methodology for a given experiment. You...

... research is about and what you think you can accomplish. No matter how many times I say this, candidates.... One of my graduate students encouraged me to publish this manual and earn some cash for my expensive...

...amassed nearly 500,000 ‘likes’, while their website lists over 200 partner organizations, and 514 satellite... is required. This is by no means an exhaustive list and every marcher is not necessarily marching for...

...in eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art...protein expression level. State-of-the-art FPs (listed in Table 1) were optimized for efficient binding...