Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 573 results for: BASE

Showing: 451 - 500 of 573 results

  1. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...difficult for individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this...

  3. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ... at present.   Another issue surrounding CRISPR-based therapy is the level of testing needed to define...

  6. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...and RNAProtect® from Qiagen, are ammonium sulfate-based solutions that work by inhibiting RNase activity...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...provide recombinant antibodies, another plasmid-based product that, with Addgene’s support, could fill...

  9. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...beyond generating frameshift mutations or single base pair edits, these enzymes are worth taking a look...Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is thus not helpful...

  16. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system that can regulate gene... is curated and added to other databases including wormbase and flybase. microPublication is run and maintained...pages and curated databases for scientists including Wormbook, Wormatlas and Wormbase. And scientists have...institute developed a 3’UTR database specifically for C. elegans. This database provides detailed information...coding mRNAs, and annotations extracted from other databases (Mangone et al., 2008).  OpenWorm OpenWorm is ...published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative data...Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737...

  17. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...used to restrict expression to a single cell type based on the overlap in cell types both promoters expressed...Institute of Molecular and Clinical Ophthalmology Basel recently took a different approach to enhance specificity...targeting the same cell class in humans (R=0.67).  A database of the microscopy images of all AAVs that reproducibly...

  20. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...you can extract practically every single reliable base pair from your results. This option is not as fast...sequence databases from the dropdown menu. Typically, we use the default nucleotide database “Nucleotide...plasmids with us or other entries in NCBI’s numerous databases. As our repository has grown over the years (we... explanation of the different BLAST programs, databases, and elements of the BLAST search pages. At Addgene...known reference sequence or to a BLAST sequence database. If you know the expected nucleotide sequence,...identity between your sequence and the reference/database sequence is ≥ 95%. [Our QC process would be trouble-free...blastx checks all six possible frames against the database. Another benefit is that a frameshift mutation...

  21. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ... target cell surface receptors (Callaway 2008). Based on this, cell type specificity of the starter cell...

  22. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...to help you learn more about fly biology and fly-based experimental systems. Some resources will direct...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington,...

  25. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...populations.   Figure 2: Compensation example based on experimental sample. Flow plots showing a sample...

  26. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of many ...

  27. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...clozapine-N-oxide, clozapine, and compound 21 in DREADD-based chemogenetics in mice. Scientific Reports 9: https...

  28. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody Resources on Addgene.org  ...

  29. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing...

  30. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to a single fluorescent...

  33. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...manager: Unlike academia, promotions in industry are based on people’s ability to manage others and work as...

  35. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature information... create their new plasmid.  The Addgene Vector Database has more information about common vector backbones...

  37. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...amino acids. This is possible because there are 4 bases (A, T, C, and G) and 3 positions in each codon. ... at all in E. coli (you can find a variety of databases showing various organisms’ codon preferences online...

  42. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available positions alone...

  46. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Lentiviral Vectors for Delivery of Gene-Editing Systems Based on CRISPR/Cas: Current State and Perspectives. Viruses...

  48. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...Learn about Non-Homologous End Joining Learn about Base Editing Read 3 Tips to Improve HDR Efficiency for...

  50. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information...we routinely wrote Python scripts, queried our database with SQL or Django, and executed code in Jupyter...
Showing: 451 - 500 of 573 results