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  5. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...provides useful information about how the vector was assembled, and enables requesting labs to utilize and modify...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...later, is it finally back in the limelight. On December 19, 2017 the FDA approved Luxturna™ the first ...

  11. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...out your Cas9? It can be! Cas12a is a Cas family member which generates sticky ends upon cutting. Cas12a...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ... Flying Spaghetti Monster; the smFP structure resembles images of the deity (see comparison image above...

  21. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...sensitive to toxicity from RNA editing, such as mouse embryonic stem cells and glioblastoma cells. PASTE Development...

  22. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...adjusted depending on the sample. It is important to remember that sample lysates will also contain proteases...

  23. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative control...

  24. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...

  25. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...resistant plasmids from Enterobacteriaceae family members within the mouse gut (Kamruzzaman et al., 2017)...

  26. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...find tables and information on different viral membranes, tropism, and advantages and disadvantages: Table...

  32. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296. (2012). PubMed Central...

  33. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...Applications: An EAMNET Teaching Module http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf...

  34. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...physically cross things out (it just feels good) and I remember things better when I write them down. Some people...

  38. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...smaller pieces, run on a gel, and transferred to a membrane for analysis through hybridization of a radioactively...

  40. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...control process. Since MiSeq involves sequence assembly from short reads (<300 bp) which cannot distinguish...

  48. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...backgrounds. In fact, many of my AAAS STPF cohort members came to DC to do either a sabbatical year or transition...

  49. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...liposome) or electrical (electric current forming membrane pores) transfection. These techniques are still...

  50. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...fact have a helicase domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation...
Showing: 451 - 500 of 510 results