...for whom English is a second or third language (making publishing their research all the more challenging...Washington in St. Louis, offer guidance on how to make a low-cost whiteboard video about your research....

.... Consultants often work with such teams to help make decisions about R&D investments in either new internal...benefits of novel drugs. Commercial Affairs teams make up a large and diverse group of professionals running...

...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

...microscopy techniques. FRET measurement sensitivity makes it suitable for studying interactions within living...approaches and appropriate controls are required to make this method useful for dynamic experiments in which...

...replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll cover the...on type of RNA transcript, you will also need to make sure your plasmid has a promoter suited to working...

... available as lentiviral plasmids if you want to make a stable cell line. This design allows highly localized...bioRxiv, under a CC-BY-NC-ND 4.0 license.   Just make sure you don’t overdo it! The moderately-enhanced...

...minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds of particles...filament expressed in astrocytes throughout the brain, making it a popular target for labeling these cells. Check...

...contributed to this post. Adeno-associated viruses (AAVs) make fantastic gene delivery vehicles for episomal gene...approach requires G418 selection of infected clones, making it unsuitable for most in vivo applications.  Third...

...recombinase. The XFPs in Flybow are membrane-tethered, making this system useful for visualization of cellular... optimize it for tissue clearing, a method that makes tissues, such as the brain, transparent so that ...

...easy, but the risk of PCR-introduced mutations can make alternative approaches more favorable if you want...restriction sites. Alternatively, if you want to make your protein non-functional, you may simply introduce...

...used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes in human induced pluripotent...alleles had unwanted insertions/deletions, therefore making those alleles useless for our studies. If one now...

...express multiple integrins that bind the same ligand, making it difficult to dissect the function of one integrin...protein research institute, partnered with Addgene to make our collection of integrin antibodies available ...

... unmethylated DNA, and more. Many mutations that make these features possible are present in most commercial...commercial strains, especially mutations that make major improvements such as those that increase plasmid...

...plasmids encoding antibodies from Addgene that you can make on your own.  To narrow your search, first pay attention...knocked down or knocked out. If you are interested in making your own knockout line for validation, please view...

... disease states, and targeting the epigenome may make certain processes, like cellular reprogramming of... likely cell type- and context-dependent, it may make sense to try multiple CRISPR tools when setting ...

...scenarios below for a CRISPR experiment which involves making specific knock-out edits in a gene(s) of interest...off-target frequency of 5%. Your experiment involves making a large sgRNA library to thousands of genes and...

...exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees... modification of interest, it would be unwise to make conclusions on the basis of the activity of a single...

...the parental animal, are rescued progeny, which makes screening extremely rapid. This stringent selection...knock-ins were occurring 24 hours post-injection, making screening for knock-ins much more efficient. Pure...

...recombination-mediated genetic engineering.  It can be used to make an assortment of modifications: insertion and deletion...with 4-5 silent changes in the wobble codons, i.e. make changes to the third base pair of the adjacent 4...

...immunoglobulin (Ig) heavy chains and two Ig light chains make up each antibody molecule. The Fc region and part...epitopes on the protein. However, each animal will make antibodies against different protein epitopes and...

...during chemical synthesis of these molecules. To make large amounts of pure product in an affordable way...constitutive and inducible promoters, linkers, and tags makes EcoFlex a great option for those interested in recombinant...

...controls needed to pressure-test your FRET assay to make sure it is working? This article addresses these...FRET experiments. However, remember that, if you make an FP fusion, you are inserting about 20-25 kDa ...

...switch from a dark state to a red fluorescent state make them useful for 2-color imaging with green PA-FPs...fluorescence usually means more photons are being emitted, making it easier to capture a clear image above background...

...ability to leverage the prestige of the employer makes EB-1Bs a very attractive option.    Your Expert ...a career search. Doing these simple things will make it easier for you to find and organize all of the...

... plateau stage after exponential amplification, making it difficult to properly analyze your data.  To...understanding how the analyses work - you do need to make sure the assumptions in your dataset match the assumptions...

... no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  in E. coli and...the solution to this inefficiency? Use CRISPR to make it a selectable process! As NHEJ is ineffective ...

...The ease and flexibility of the LACE technology makes this system widely accessible for many potential...interest within the INT location. One plasmid to make note of is the INT construct bearing the "Bunch ...previous studies, and provide new insights into the make-up of multiprotein complexes like the EPC1 complex...

... While helpful from a biological standpoint, it makes delivering AAVs to the brain difficult. Traditionally... References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & Chaudhari, N. (2022). Selectively...

...vectors for many experimental purposes. To help you make sense of all the viral vector information that's...

... on the Addgene blog Learn more about Blugene's make-over Get a how-to guide on the new Addgene search...

...section and additions describing CRISPR’s ability to make large genome edits using transposases or the Cascade-Cas3...

...names as finalists. Now, we’re asking you to help us make the decision between some meaningful names…. Watch...

...possible for us to reach everyone. Therefore, to make our library more useful to our community, we’re ...

...NEB's DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan...

...and T shirt size. You have until April 7, 2021 to make your way through the scavenger hunt and enter the...

...Higher and Deeper” by Jorge Cham   2)   “Boxplot” by Maki Naro   3)   “Bird and Moon” by Rosemary Mosco   ...

...pipette boxes, wire, cable ties) received a festive makeover today✂️❄️🦠thanks to the awesome templates by ...

...We’re making some moves over here at Addgene...and they’re looping? What’s the deal with looping...

...Purpose of Science Communication #1 Mistake Scientists Make when Communicating with Non-Scientists How to Approach...

... depositors here at Addgene — your contributions make the repository what it is. So we’re thrilled this...

...being used for bacterial genome engineering. To make it easier for you to find plasmids for your work...

...and YouTube channel. The goal of this page is to make it easier for you to find content relevant to your...

...environmentally responsible manner.  Other ways to make the office more eco-friendly The switch to 100% ...

...suggestions here or by using the hashtag #AntibodyMakesThree on Twitter. As with Blugene and Aavery, we...

...Editors: Modified versions of CRISPR nucleases that make single base changes in the DNA sequence without ...

...CRISPR X from Michael Bassik's Lab - A new tool for making diverse, localized point mutations. Listen to Our...

...news (pop culture events of the past year obviously make for good costumes), science and our plasmid collection...

... the community is your negative data. We want to make sure people are selecting AAVs that will work for...

...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...

...your viral packaging service in the same place, making it easier to go from selection to viral vector....