We narrowed to 481 results for: mme

1. Thoughts on the Future of Research 2015 Boston Symposium

   Type

   Blog Post

   Published

   Nov. 25, 2015, 4 p.m.

   ...develop a plan to make postdoctoral pay/benefits commensurate with work training and experience, better align...

2. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   ... negotiating budgets and invite you to leave a comment below Happy Sciencing, Damien   Damien Wilpitz...

3. Hot Plasmids: Spring 2025

   Type

   Blog Post

   Published

   June 3, 2025, 1:15 p.m.

   ...PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments. These new...

4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

   Type

   Blog Post

   Published

   Jan. 16, 2025, 3:42 p.m.

   ...system. As we cannot make universal serotype recommendations, the following table focuses on tissue tropism...

5. CRISPR 101: Off-Target Effects

   Type

   Blog Post

   Published

   Feb. 8, 2024, 2:15 p.m.

   ...localizes to unintended sites and performs its programmed function there, this is an off-target effect....

6. Viral Vectors 101: Optogenetic Tools

   Type

   Blog Post

   Published

   June 27, 2023, 1:15 p.m.

   ...help light your path! Resources and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic...

7. Plasmids 101: Repressible Promoters

   Type

   Blog Post

   Published

   Dec. 20, 2022, 2:15 p.m.

   ..., the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 1988 Jun;2(6...

8. Antibodies 101: The Basics of Western Blotting

   Type

   Blog Post

   Published

   March 8, 2021, 2:41 p.m.

   ...transfer as well using this method.  Finally, commercially available dry blotting systems efficiently transfer...

9. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...

10. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...the highest fidelity NAD+-dependent DNA ligase commercially available (see below). Conclusion It is important...

11. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type

    Blog Post

    Published

    Nov. 2, 2021, 1:15 p.m.

    ...serotype best transduces your cells of interest. We recommend testing several different serotypes to determine...

12. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ...infect. Most of the protocols you will find will recommend using a polycation, like polybrene (protocol) ...

13. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type

    Blog Post

    Published

    March 7, 2017, 3:30 p.m.

    ...pools of Cas9-A549 cells at an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use ...

14. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    Published

    Feb. 25, 2020, 2:15 p.m.

    ...engineering company, Genentech, was founded.  This commercialization of gene editing technology led to the development...

15. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    ...Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T, Bantscheff M, Steinmetz LM, Drewes G, Huber...

16. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...containers. Clean up any media or culture spilled immediately. Water baths, liquid nitrogen containers, reagent...

17. Finding Your Science Policy Path

    Type

    Blog Post

    Published

    Sept. 22, 2020, 1:15 p.m.

    ...boards, support ballot initiatives, deliver public comments, meet with local representatives, or even run ...

18. Sequencing Options for CRISPR Genotyping

    Type

    Blog Post

    Published

    Oct. 4, 2016, 2:30 p.m.

    ...profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature biotechnology...

19. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ... C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes...

20. Plasmids 101: Modular Cloning Applications and Kits

    Type

    Blog Post

    Published

    May 14, 2024, 1:15 p.m.

    ...   Figure 1: A chart with MoClo kit recommendations for different modal organisms and applications...

21. Antibodies 101: Flow Cytometry

    Type

    Blog Post

    Published

    July 20, 2021, 1:15 p.m.

    ...arising from the secondary antibody.  It is also recommended that users include a viability control as this...

22. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ... antibodies for neuroscience research from the Trimmer lab ...

23. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    Published

    March 14, 2017, 2:30 p.m.

    ... effector function. Cancer cells also produce programmed cell death ligand (PD-L1) that inhibits the cytotoxic...

24. Which Fluorescence Microscopy Techniques is Best for Me?

    Type

    Blog Post

    Published

    Oct. 10, 2017, 1:57 p.m.

    ... glass and the water-like buffer the sample is immersed in. Although the excitation light is completely...

25. Selecting Your Plasmid Purification Kit

    Type

    Blog Post

    Published

    May 27, 2025, 1:15 p.m.

    ...viability. Avoid Low Quality/High Quantity – We recommend avoiding a High Quantity/Low Quality mindset for...

26. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nature Biotechnology...

27. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...easily isolated somatic cells that have been reprogrammed to iPSCs successfully [10-12]. The next stage...

28. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...of promise for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first...

29. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type

    Blog Post

    Published

    Sept. 3, 2020, 12:15 p.m.

    ...engineering in 2012.  Many of these advances have stemmed from the goal of reducing off-target Cas9 activity...

30. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    Published

    Sept. 28, 2020, noon

    ...10.1016/j.cell.2013.02.022 Ronda C, Pedersen LE, Sommer MOA, Nielsen AT (2016) CRMAGE: CRISPR Optimized...

31. 28 Hot Plasmid Technologies from 2015

    Type

    Blog Post

    Published

    Dec. 23, 2015, 3:30 p.m.

    ...from the past year? Let us know about it in the comments or shoot us an e-mail at [email protected] and ...