We narrowed to 488 results for: mme

... antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest project to develop...

...should validate it for your particular setup. We recommend starting by searching the literature to see what...

...10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based ...

...performance. By this point, towards the end of the summer, things were getting better and the office had ...

...with all plasmids in the repository, we highly recommend reading the associated publications or protocols...

... have any questions, please write to us in the comments section.  FX cloning kit This kit is designed...

...extensively worked with and optimized Sep-OTSλ and can recommend its use (6).  2. C321, a specialized bacterial...

...develop a plan to make postdoctoral pay/benefits commensurate with work training and experience, better align...

... negotiating budgets and invite you to leave a comment below Happy Sciencing, Damien   Damien Wilpitz...

...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...

..., the trans-activator of herpes simplex virus immediate early gene expression. Genes Dev. 1988 Jun;2(6...

...PDGFR and NGR fusions for membrane display, they recommend the NGR vectors for most experiments. These new...

.... Though we cannot make universal serotype recommendations, the following table summarizes tissue tropism...

...localizes to unintended sites and performs its programmed function there, this is an off-target effect....

...help light your path! Resources and references Recommended reading Abreu, N., & Levitz, J. (2020). Optogenetic...

...transfer as well using this method.  Finally, commercially available dry blotting systems efficiently transfer...

...the highest fidelity NAD+-dependent DNA ligase commercially available (see below). Conclusion It is important...

...Grandi P, Jakob P, Michon A-M, Sun H, Tessmer K, Bürckstümmer T, Bantscheff M, Steinmetz LM, Drewes G, Huber...

...containers. Clean up any media or culture spilled immediately. Water baths, liquid nitrogen containers, reagent...

...boards, support ballot initiatives, deliver public comments, meet with local representatives, or even run ...

...infect. Most of the protocols you will find will recommend using a polycation, like polybrene (protocol) ...

...pools of Cas9-A549 cells at an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use ...

...serotype best transduces your cells of interest. We recommend testing several different serotypes to determine...

...engineering company, Genentech, was founded.  This commercialization of gene editing technology led to the development...

...profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity." Nature biotechnology...

... antibodies for neuroscience research from the Trimmer lab ...

... glass and the water-like buffer the sample is immersed in. Although the excitation light is completely...

... effector function. Cancer cells also produce programmed cell death ligand (PD-L1) that inhibits the cytotoxic...

...arising from the secondary antibody.  It is also recommended that users include a viability control as this...

... C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes...

...   Figure 1: A chart with MoClo kit recommendations for different modal organisms and applications...

...easily isolated somatic cells that have been reprogrammed to iPSCs successfully [10-12]. The next stage...

...viability. Avoid Low Quality/High Quantity – We recommend avoiding a High Quantity/Low Quality mindset for...

...profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nature Biotechnology...

...of promise for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first...

...engineering in 2012.  Many of these advances have stemmed from the goal of reducing off-target Cas9 activity...

...10.1016/j.cell.2013.02.022 Ronda C, Pedersen LE, Sommer MOA, Nielsen AT (2016) CRMAGE: CRISPR Optimized...

...from the past year? Let us know about it in the comments or shoot us an e-mail at [email protected] and ...