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...Addgene blog. In fact, we have so many blog posts about careers that we’ve compiled our top career blog ...wait until you need a job to meet people and learn about what's out there.”- Joanne Kamens, Executive Director...school, I came across Joseph Campbell, and read about his "follow your bliss' mantra and never forgot ...Usually when I see inspiring artists who are thinking about getting into the arts (or any career to be honest...follow your passion. Do what you love. Don't worry about the rest.”- Jason Snair, Product Designer On the...

...ways scientists have adapted them for use in the laboratory. The discovery of GFP The story of how fluorescent...particularly interesting. Although humans have known about bioluminescent organisms and their characteristic...sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms...studying it grew. In particular, studies in the laboratory of UCSD biochemist Roger Tsien resulted in much...for you, so if you’re interested in learning more about fluorescent proteins, I invite you to download our...

...well-integrated into our lab lives. Read on to learn about some creative approaches to lab sustainability, ...for rerouting waste from the landfill, but what about all the manufacturing steps that happen before you...ACT label, which is an eco-nutrition label for laboratory products that emphasizes Accountability, Consistency...away your FACS machine after a single use. So how about washing and reusing pipette tips in a high-throughput...Additional resources on the Addgene blog Learn about recycling initiatives for the lab Subscribe to the...

...attracting so much attention. When I spoke with Daniel about the interplay of these two ideas he said “science... scientists sometimes forget how to communicate about their work with non-academic people.” Daniel went...perception, which is ironically what science is about. For me it is essential to stay in touch with the...unique samples, meaning that we know very little about these specimens - especially those which are now...Additional Resources on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources on...

...Addgene blog Learn about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty...DNA transposons are most commonly used in the laboratory for genome manipulation. When transposons are...Applications of transposons Now that you’ve learned about some of the popular transposon systems, let’s take...2020) Jump around: transposons in and out of the laboratory. F1000Res 9:135. https://doi.org/10.12688/f1000research...Beauty DNA transposon Learn about the piggyBac DNA transposon ...

...a summary snapshot without telling you anything about outliers or data spread within those data sets. ...the rules” and which we have certain information about. The assumptions and requirements that need to be...Quantitative If your data doesn’t fit the requirements above, a non-parametric test may be the way to go, which...only performing the comparisons you really care about. Family-wise error is the probability of a false...rate than the multiple comparison tests described above. Violated assumptions and statistics Statistical...not meet the assumptions for the tests described above (normal distribution, equal variance, and independent...

...new plasmid tool from the past year? Let us know about it in the comments or shoot us an e-mail at [email protected] and maybe we can write a blog post about it! Split Cas9 system The Cas9 protein is composed... lentiviral expression (MuLE) system from the laboratory of Ian Frew simplifies the process of creating... seem preposterous. Luckily, the Pierre Neveu laboratory designed a new iterative, chaining-based cloning... to improve on the Arch indicator and their collaborators in Robert Campbell’s lab carried out five rounds...improved light-switchable bacterial TCSs Jeffrey Tabor's lab has developed new synthetic biology tools by...for controlling gene expression. For more details about these light-switchable TCSs, read our blog post:...

... of Bioinformatics reached out to us to tell us about a resource called Cellosaurus, a growing cell line...all the information in the resource.  This is a labor-intensive project; scripts can be used in some cases...preferences to help you identify proximal lines you care about. If you have cell lines without a certified origin... Or finding errors in the available information about a cell line you’ve used? Submit your manuscript ...distributor. A success all around! If you’re excited about this resource and want to learn more, check out ...

... for bag check was definitely worth it! Hearing about his passion for global health and the various initiatives...with fluorescent microbes. Check out her animation about the Hawaiian bobtail squid!  Continuing the art...talked to some scientists who stopped by the booth about amazing work already represented in the Addgene ...opportunity for Addgene to let microbiologists know about our mission of open science and resource sharing...community, contact [email protected] to find out more about our free deposit and distribution service! Did you...

...It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...CRISPR technology in just 2 years and excitement about how much more there is left to do with the characterization...downstream output. Tuesday morning's talks were also about circuitry and synthetic biology, and I found myself...plasmid sharing to those who were anxious to hear about what was coming next. I also got to show off our... updated Synbio page to people who were curious about these newest features of our website.  I want to...

... your science with others.    My favorite quote about science ever comes from an interview in which someone...storytelling business like to say, science is all about navigating conflict. In literary terms, conflict...find that once I sit down and talk to a scientist about their research, these conflicts come out quite naturally... a simple question, like “Have you ever thought about how X works?”, or even by introducing a person that...devices, we sometimes forget the most important thing about science: It’s cool. Like bowties! Using science ...

...our Summer SciComm Series, where we’ll be talking about science communication, best practices in the field...research.   This summer, we’re making time to talk about my favorite thing - science communication (scicomm...endless.    Activity Example audience  Talking about your career as a scientist underrepresented minorities...students in AP biology classes Creating a podcast about genetics using dog breeds as an example adults ...get into the more exciting parts - learning more about science communication itself! See you next time!...

... also be collecting feedback from the community about how the antibodies work in various applications,...antibodies are plasmid-based and recombinant. Learn more about recombinant antibodies here. Finally, Addgene is...against PSD-95 from the Trimmer Lab. Information about the antibody, the target antigen, and performance...blog and Antibody Guide, which include information about immunofluorescence, conjugates, isotypes, and more...learn more or get started.  If you have suggestions about how to make the resource better or ideas for new...

...Twitter, you might have seen the video of her singing about the paper recently published by her lab and Mustafa...professor at the University of Freiburg. This paper is about a new tool called BLADE (blue light-inducible AraC....   Inspired by @UriAlonWeizmann, here my song about our paper recently published in @nchembio Title:... based on the concept of bacteriographs created about a decade ago. To create these images, the scientists...Additional resources on the Addgene blog Learn more about inducible promoters Visit the Plasmids topic overview...

...living as a scientist in Beijing, talking to people about taking beautiful photographs of their studies, reporting...to friends or dates or colleagues we tell tales about parts of our lives that tend to have a story structure...informational interview question: Interviewer: “Tell me about a time you had a conflict at work.” Interviewee:...situation down by opening a dialogue specifically about the project and contributions, rather than getting...defensive or getting into ‘he said; she said’. We spoke about it for a while and he eventually calmed down, apologised...

...section-specific comments, I re-read what I wrote about the paper after my initial read, adjust my language...wish for The last thing that I wanted to mention about the peer-review, is that as a peer-reviewer, you...each peer-review, I try to mention what I liked about this paper and recognize the hard work that the .../visualization using R/Shiny. She is passionate about open science, BIG data analysis and sharing whatever...review article Resources on Addgene.org Learn about depositing plasmids pre-publication Find molecular...

...We are excited about our new partnership with Allele Biotechnology which allows researchers to deposit...blue-shifted compared to its parent protein CFP484. Collaborating with scientists from Florida State University...mWasabi plasmids here! One of the most notable things about these proteins is that they are monomeric fluorescent... fluorescent protein blog posts Find blog posts about choosing fluorescent proteins for your research ...the Addgene blog Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection...

...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

...focus on molecular pathways, I found myself curious about other routes – In terms of both types and topics...not resist applying for. Not that I knew anything about being an editor - that I learned on the job. The...science? Look at our graphics and design editors. How about condensing a week’s worth of the world’s scientific...the scientific publishing world and debate issues about how to improve the scientific publishing process...science communication professionals who are thinking about, for example, how to use new technologies to reach...

...frequency of use in black. This paper talks more about the development of the De-jargonizer.   Academic...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...episode 2 "Organizing Literature" : I found out about Zotero here Dear Grad Student podcast episode 2 ...Programming, & Productivity": This podcast talks about Mendeley, Zotero, and other citation managers and...the pomodoro technique": This podcast talks more about using the Pomodoro method in grad school Additional...