...Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project Advice... to get advice on choosing a lab, picking your research topic, starting a lab, and more. Before we start...others out, they'll help you too; both with your research and with getting published.” Connie Cepko 1....interested in leaving academia should present their research at conferences to obtain a “crash-course in all...tuned for the follow up blogs on "Choosing Your Research Project," “Advice for Starting Your Lab,” “Fun...

...biomedical research". The family entered the biomedical field’s spotlight after researchers discovered...animals have impacted humans through biomedical research. The Camelid family is composed of camels, llamas... llamas and alpacas (Figure 1). At the time, researchers were already on a quest for smaller forms of ...experiments, due to their simple structure    sdAbs in research These advantages have contributed to sdAbs becoming...variable domains (aka nanomice), which gives researchers the “best of both worlds” - a familiar lab model...

...Plasmids from a variety of research labs and hopes that these will help researchers build their knowledge ...efficiently in humans. This is why we cannot stop searching for novel solutions to treat rare diseases like...causes, and potential cures. We encourage any researchers working on ACVR1 or any of the pathways that ...about Addgene's Previous Work with Rare Disease Researchers Check Out Potentiall HIV Treatments with CRIPSR... Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial Collections Page ...

...Fluorophores In this post, Luke Lavis from Janelia Research Campus reminds us that, although fluorescent proteins.... To learn how you can use minigenomes in your research, check out this post! R Bodies: Membrane-Rupturing... post, Jessica Polka, formerly a postdoctoral researcher in the Silver Lab at Harvard University, describes...Genome Editing CRISPR has revolutionized how researchers make knockouts and other types of genome edits...In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab, describes how you can better...Addgene Blog useful resources for the biological research community! ...

...Arabidopsis research and teaching community through the TRAINED initiative (Translating Research on Arabidopsis...Ohio, ABRC ships more than 100,000 samples to researchers and educators in 60 countries each year. Introducing...Arabidopsis Arabidopsis is an important model system for research and is used by scientists around the globe. As...Educational Resources). Arabidopsis is used in research to explore a variety of phenomena, and so too ...Park Conservatory. Do you use Arabidopsis in your research? Are you a biology teacher or professor? Whether...

...job specifically looked for a researcher with 3 years+ of cancer research experience, a PhD or a Master...what leaving academia feels like for a lot of researchers. For many years I struggled with the questions...lie ahead of me? Like a considerable number of researchers, I had high hopes of securing tenure in a British...time in the lab, my colleagues and what I was researching, but I decided I needed a new challenge. Looking...widely available, especially for life science researchers. Many biotechnology companies are looking for...

...modular approach made ZFNs highly appealing to researchers.  TALENs In 2010, transcription activator-like...at introducing point mutations. In 2016, the researchers at the Broad Institute envisioned a new strategy...consider the ethics and broader implications of our research.  Want the Genome Editing Trading Card seen in...understanding of the base editing enzymes. Kartik’s research focuses on the use of molecular dynamics simulations...our CRISPR guide Find CRISPR plasmids for your research Browse plasmids for base editing References Anzalone...PJ, Wilson C, Newby GA, Raguram A, Liu DR (2019) Search-and-replace genome editing without double-strand...specificity of a homing endonuclease. Nucleic Acids Research 30:3870–3879 . https://doi.org/10.1093/nar/gkf495...

...remarkable technological advances have taken researchers to the threshold of a revolution in modern neuroscience... channelrhodopsins, the first two found in the Archaea, the third in green algae (Oesterhelt and Stoeckenius...In order to express opsins within the brain, researchers inject genetically modified viruses encoding ... functional topography of the neural circuit architecture of the brain regions associated with motivated... in pigmentation." Biochemical and biophysical research communications 78.1 (1977): 237-243. PubMed PMID... Chris, and Kimberly R. Thompson. "Overview on research and clinical applications of optogenetics." Current...development: the power of optogenetics for epilepsy research." Frontiers in cellular neuroscience 12 (2018)...

...Eckhardt and Melanie Brewer from the QBI Coronavirus Research Group at UCSF. It’s been only a few months since... more than 20 labs to form the QBI Coronavirus Research Group (QCRG) to do just this.  In under a month...with requests to share our plasmids with other researchers. We have been excited to see so much interest...problem more quickly. Figure 2: Getting our research in line, with a row of FedEx envelopes waiting...shared spreadsheet and it takes time away from our research. For all these reasons, the QCRG is delighted ...requesting plasmids for coronavirus or COVID-19 research, please email Addgene at [email protected] with...

...scientists will choose to start their own academic research laboratory. How does one even go about starting... But how does one build a strong collaborative research team motivated by similar visions and goals? We...the lab. “An effective working environment for research is totally dependent on communication amongst ...for some quick advice and resources for hiring research staff. And for a more indepth resource for starting...departmental administrator, lab and safety manager, researcher, adviser, conference planner, and writer.” Connie...: Running Your Lab (pdf) Advice for Choosing a Research Project 9 Tips to Achieve Success in Academia  ...

...needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully complete...GCE provides almost endless possibilities for researchers, from selective protein ligation through introducing...literature into robust and universal techniques that researchers can easily implement in their own labs. They ...working to make GCE available for all biomedical researchers. If you’re interested in using GCE in your experiments...protocols would be broadly useful for biomedical researchers. One way of doing that is by using Addgene’s ...would be useful. Think GCE could be useful in your research? We definitely recommend checking out GCE4All’...

... money to support this research. But there is a move towards more open research. The EU, for example, ...community to create state-of-the-art devices for research. There are a number of new platforms (such as .... Direct sharing of work from universities and research centers provides a potential conflict between ...can lead to faster and more efficient science. Researchers around the world don't have to start from scratch...“openUC2” and focuses on bringing cutting-edge research to everybody by relying on tailored image processing...

...adapted CRISPR nucleases for many more specific research needs. In this update, we will focus on the most... variants may be appropriate for your specific research question. Considerations when choosing your Cas...recognize a variety of different PAM sequences. Researchers have also used experimental evolution to develop...that may make it especially suitable for in vivo research and potentially treatments. Cas12b, characterized...al., 2018) Labs also shows promise for in vivo research due to its small size and specificity relative..., it is likely that the toolkit available to researchers will continue to expand. Final thoughts Since...

...provide a much needed boost to basic molecular plant research, more initiatives are needed to increase the body...Organizations like Open Plant can then build on this research and effectively carry out their mission of creating...selected a project in a field with 30 years of basic research standing behind it. Our color changing flower ... Petunias have been a model organism for plant research for decades, and the biochemical pathways involved...circadian rhythm in petunias. Because of the extensive research that has come before us, it’s possible for us ... flowers that change color. Without this basic research – this comprehensive understanding of the genetic...

...technology that’s already had a huge impact on research and will be affecting our everyday lives very ...visualizing genomic loci, and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved...effects, respectively. Recognizing these issues, researchers from the Zhang lab at the Broad Institute, the...structure leads to the development of HypaCas9 Researchers in the Doudna Lab at Berkeley recently added ...eSpCas9, SpCas9-HF1, and HypaCas9 should enable researchers to make precise edits in mammalian cells and ...question very soon! We look forward to seeing how the research community makes use of these tools and are excited...

... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...recombinant viruses typically used in neuroscience research (e.g., AAV) are useful for labeling the individual...recombinant forms of these viruses typically used in research aren’t able to replicate, and for a virus to cross...receptor (TVA) in user-defined cells. For example, a researcher can deliver Cre-dependent TVA to Cre-expressing... (i.e., it is taken up by projection neurons). Research into these phenomenon has shown that the RABV ...

...Principle Investigators (PIs) - whose labs does research that aligns with your interests and goals.  Reaching...gives a more comprehensive idea of that advisor’s research interests which can be helpful in creating your...where you can further discuss aspects of the lab, research, and provide them with a CV or transcripts if ...interests, but will ask you questions about your research goals and education, too! Prepping for the interview...quickly reference your questions about the PI’s research or the name of the award you won for your conference... student at George Washington University whose research focuses on Drosophila-parasite pathogen interactions...

... more than 10 modules often rely on two-step hierarchical approaches using different Type IIS restriction...Development of Golden Gate Assembly test systems Our research focused on three different levels of assembly:...number of transformants. To compensate for this, researchers can plate larger volumes of the outgrowth containing... to minimize this possibility. Recently, NEB researchers profiled the comprehensive fidelity of cohesive...important tools for many areas of science, and researchers continue to test the limits of DNA assembly approaches...and bias during DNA end-joining." Nucleic acids research (2018). PubMed PMID: 29741723. PubMed Central ...

...and professional development opportunities for researchers interested in connecting with the public. My ...viewing my activities as types of experiments. I searched for new opportunities with an open mind and open..., funny, picture-filled blog posts about their research? Then we could get everyone to share their articles...This seemed like a great way to share some of the research taking place at our university with the community...resource that has gained a lot of traction with researchers. It is an excellent way to meet people with similar...Resources on the Addgene.org Find Plasmids for Your Research Learn About A Variety of Different Fields on Our...

...request activity around March. A rough estimate shows that activity level in March was around 33% of that... the rest of the world, from mid-January to mid-March.  To obtain a better view of how plasmid orders ...our distribution, resulting in the following hierarchical model: Because the U.S., Europe, and China ...University. Max Shen is a Ph.D. candidate at MIT. His research uses applied machine learning and statistical ...Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution to illuminate...