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We narrowed to 577 results for: BASE

Showing: 181 - 200 of 577 results

  1. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...of imaging data (ILASTIK), or the popular plugin-based image process framework FIJI are just three of the...to the biological protocol, and assembly designs based around the use of commercially available tools and...provides a potential conflict between traditional IP-based models and the use of taxpayer money to support ...

  2. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...allows classification of individual target molecules based on their mutation status. The drop-off probe is ...References 1. Miyaoka, Y. et al. Isolation of single-base genome-edited human iPS cells without antibiotic..., Berman, J. R. & Postovit, L.-M. A Digital PCR-Based Method for Efficient and Highly Specific Screening...

  3. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...contributed by guest blogger, Rachel Rubinstein, a field-based software product manager at Oxford Nanopore Technologies... messy if you’re not on top of it. In a science-based software PM role, having a good understanding of...Rubinstein! Rachel Rubinstein is currently a Field-based Product Manager at Oxford Nanopore Technologies ...

  4. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... efficiencies ranking as low, medium or high is based on the reprogramming efficiencies reported in González...Non-replicating Plasmids: Generating iPSCs with plasmid-based expression requires serial transfection of 1 or ...Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids carry the origin of replication...DNA methylation. Another downside of using EBNA1 based vectors is that the plasmid can hang around for ...behave. iPSCs are typically tested with molecular-based assays first. These methods less rigorously assesses...Transgene Expression Mediated by a Sendai Virus-Based Vector Using a Nonstructural C Protein and Endogenous...

  6. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    Published
    June 16, 2020, 1:25 p.m.
    ... of DNA you need, and at which level of purity. Based on these premises we can classify a plasmid preparation...will adopt for the extraction. Restriction enzyme based approaches can require several hundreds of nanograms...

  7. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ... your website? Many vendors will process orders based on a purchase order, which is an official document...depositing institutions. Addgene cannot fulfill orders based on PO documents that are sent to us by fax or email...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...resistance cassette) in pUC19 based entry vectors, as well as the pGreen-IIS based destination vectors. To ...CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing a means...ACS Synth Biol 2013 Sep 20;2(9):537-41. DREADD-based chemogenetic technologies After several years...up to six DNA fragments at a time. This method (based on the Golden Gate technology) exploits the ability...reconstitution across synaptic partners) technique based on functional complementation between two non-fluorescent...tags in the FP sequence for separation from Cre-based Brainbow-expressing cells. Autobow plasmids are ...The expression plasmids for a given species are based on the same backbone to permit expression levels...

  9. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...is less than half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging...was shown to be effective for CRISPR activation, base editing, and genome editing in mammalian cells. ...had the highest activity for gene activation  and base editing, whereas a different set of mutations (the...

  10. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...protein degradation. Enter TRIM-Away - an antibody-based system developed by the James and Schuh labs. Clift...endocytosis is further complicated by the reliance on pH-based biosensors used to detect the maturation of neutral... Crabtree’s lab has developed a new CRISPR/Cas9-based system to study epigenetic modulation. While catalytically...

  11. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...recombination of the targeting vector. CRISPR/Cas9-based systems have been developed for generating knockin... for gestation. All three can introduce multiple base pair deletions, but CRISPR/Cas9 can also introduce...in which the conditional recombination can occur based on the cells in which Cre is expressed.  Several...

  12. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...change, so they are typically used in microscopy-based assays like immunohistochemistry, though they are....  Biotin Biotin is used in a range of antibody-based assays. Biotin itself is not visible or capable ...But hopefully, this introduction provides a good base from which to dive deeper!References and resources...instruments, each other, and your target. I love FPbase’s Spectra Viewer tool for comparing different fluorophores...

  13. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...resolution for in vivo work. APEX2, a faster peroxidase-based labeling approach, is an alternative option but ...signaling. Limitations of RaPID Like other BirA*-based proximity labeling methods, RaPID cannot distinguish...reported by other research groups that performed BirA*-based affinity purification mass spec experiments. These...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...gradient with different concentrations of your protein-based buffer (FBS, milk, etc.) against the same amount...instead of concentrations. Most labs use protein-based buffers, so here’s a quick tip: Keep a bottle of...even give gelatin a shot.  Remember that protein-based blockers degrade fairly quickly, even at 4 °C, so...peroxidase protein (HRP), have a limited dynamic range based on the solution used to expose them. You’ll need...(if available) and note in analysis Plasmid-based proteins: plasmid sequence not accurate Check ...and/or concentration Try a different protein-based buffer (eg, milk instead of BSA) Nonspecific...buffer/adjust lysing buffer strength Sonication based lysing protocol Subcellular fractional kit or...

  15. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...diffusion rates. Smaller genetically encoded probes are based on cofactor binding proteins as described above....the His tag and several others. Another peptide-based strategy uses an enzyme to covalently label the ...: 9759496.  3. Buckley, Anthony M., et al. "LOV-based reporters for fluorescence imaging." Current opinion...PMC3129320.  18. Mastop, Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in single...protein are available (Cranfill et al., 2016 and FPbase.org). A number of variants remain fluorescent under...

  16. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...expression of many different genes was predictable based solely on the promoters and Shine-Delgarno sequences...Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end or paired-end...segments that can be engineered to bind specific RNA bases. By swapping out these different modular repeat ... the variant PUF segments required to bind any 8 base RNA sequence. The segments are provided in Gateway...

  17. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...Medical School/Brigham and Women’s Hospital (BWH). Based on her experience co-directing Mentor Circle Programs...-Europe professional collaborations, setting up bases and contacts with Spanish laboratories to start ...key to the success of this program. As we build a base of former mentees, we encourage them to get involved...consulting firm focused on new health care models based on integrative and eco-social health strategy, and...their degree. Students filled in a pre-program, baseline self-assessment survey to determine their expectations...expectations of the program and their baseline skills. Mentors were Spanish scientists (MD, PhD and MD/...defining expectations of the program and their baseline skills and a post-program self-assessment, participants...

  20. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ... after I arrived, I was looking through a paper-based, affordable, and portable microscope called the ... known as gas vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial...
Showing: 181 - 200 of 577 results