...can wrap the membranes into stacked structures including organized smooth endoplasmic reticulum (OSER) ...assays used in the papers describing the FPs above including molecular sizing columns and native gels fail ... Anatomy and Structural Biology. His interests include the quality control of secretory proteins in the...

...be relied on exclusively because historical studies of pathogen research have conclusively shown that barrier...For example, an sgRNA-only drive will spread exclusively through populations that already express Cas9...

...this, scientists use a multitude of techniques including co-transfection of two or more plasmids, the use...course multicistronic vectors do not have to exclusively be used as a means of detection; they are useful...note that IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized 2A and...

...terrific getting-started guide for BLAST, which includes a simple explanation of the different BLAST programs...regions” filter unchecked so that these regions are included in the search to maximize the alignment length...sequence alignments that BLAST is unable to handle, Clustal is our frequent choice for pairwise or multiple...

...addressed in a somewhat cursory manner, their inclusion here adds much needed context to understand every...state of science would be complete without the inclusion of some interesting case studies, and Zimmer provides...who originally isolated the GFP gene but was not included amongst the Nobel prize winners for developing...

... different.  Conclusion Figure 7: Mean inferred effects by region. In conclusion, our model infers...requests correlate with paper publication The dataset includes 11,426 plasmid requests over 6 years for 35 papers...

... Selecting crispr in Guides will return links including our CRISPR guide.  For a closer look at this new... deposit prior to publication and were able to include specific plasmid ID numbers. If you know the ID...suggest direct links to other types of searches including depositing labs and institutions. For example,...

...current genome engineering applications of CRISPR including: generating single or multi-gene knockouts in ...) that harbors two surface exposed cysteines, including the native C547, allowing for ligation to pyridyl...Senior Scientist at Addgene.  Andrew's interests include genome engineering and microbiology.     References...

...mind that access and availability to resources (including the internet!) can vary depending on factors like...your best to understand what kind of resources - including your time - will be required to produce an outcome...naked eye or to make things more accessible by including, highlighting, or omitting details. Digital or...

...viral vectors in the lab. Advantages of lentivirus include a large genetic capacity and the ability to transduce...CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: lentiCRISPR... observed in small trials for other diseases, including the common hematopoietic disorders sickle cell...

... and management process or the whole process, including grant readiness, funding opportunity research,...grant training, mentoring and coaching services, including Smartegrants, to help those entering the Grants...LLC, a grant professional development firm that includes the social media community GrantChat. She has ...

...improvements? You could publish a paper that happens to include your improvements, or maybe even a methods paper...variety of useful molecular cloning protocols, including making LB agar plates, restriction digest of plasmid...been known to recommend protocols listed on OWW, including those for preparing your own competent cells. ...

...scientists employ GFP for a wide number of functions, including: tagging genes for elucidating their expression...detect a variety of intracellular conditions, including ion (such as Ca2+) concentrations and pH, using...selection: Expression constructs like plasmids often include GFP as a marker to help identify which cells have...

...proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance ...can be used for an array of detectable proteins including fluorescent proteins, antibiotic resistance, and...

...replication control that are not necessarily mutually exclusive. In the first, the replication initiation factor...Asymmetric plasmid loss occurs for several reasons, including one plasmid blocking replication of another and...incompatibility caused by random assortment of pure plasmid clusters. Molecular Microbiology 56:1430–1440 . https:/...

...previously not associated with ricin regulation, including almost all genes involved in the production of...non-targeting guides and 6,750 safe-targeting guides were included in the library. Safe-targeting guides were designed...activity, Morgens et al drew some generalizable conclusions about off-target activity, demonstrate safe-targeting...

... bioinformatics pipelining In many use-cases, including ours at Addgene, the overall purpose of a data...workflow management system for scientific pipelines, including bioinformatics. We loved the parallels between...language.  The modern architecture of Nextflow included built-in support for containers, such as Docker...

...many types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA libraries...viruses are sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests specifically...aliquot to full use! Common conditions to optimize include incubation time, antibiotic conditions, and amount...

...If you choose this strategy, it’s important to include the proper protein expression elements (ribosome...entry clones available for many popular genes, including Hs.KRAS4a. Use our website to search for your ...Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression Systems...

...N-glycosylase (UNG) enzymes. Various other proteins included in different CGBE tools allow the researcher to...again, this post has a lot of acronyms, so we've included a glossary at the end of this post! Figure...for their application from among many options.  Conclusion With these advances, every base can now be directly...