...Each system also includes Tn7-like transposase components. Type I (generally) includes tnsA , tnsB , tnsC...applications to increasingly complex functions, including the ability to selectively activate/repress target...carried out by Cas9. Multiplexing applications include editing multiple genes at once; using dual nickases...increased fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions between the...SpCas9-derived variants with non-NGG PAM sequences include: xCas9 - NG, GAA, and GAT; increase nuclease fidelity...adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and...members inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition; inhibiting...

...plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) Toolkit includes a modular...specifically designed for use in plants. These resources include tools for research using plant systems such as ... to fluorescent proteins. Addgene’s collection includes plasmids containing promoters that drive general... few examples of useful plant CRISPR resources include: A Multipurpose Toolkit enables targeted, specific... Chen. Cas9 and gRNA backbone for use in rice. Includes a protocol (Link opens in a new window) for Targeted... Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones...Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding...

... of neurons. Common neurodegenerative diseases include Alzheimer’s, Parkinson’s, ALS, and Huntington’s...degeneration of nerve cells in the brain. Symptoms include changes in behavior and emotion, uncontrolled movements...is the most common cause of dementia. Symptoms include memory loss, deficits in decision-making and language...Addgene's collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. ... general and neuroscience -related antibodies including: Anti-KChIP1 K+ channel [K55/7R] Anti-Fig4/Sac3...Addgene's collections of fluorescent proteins, including Markers of Subcellular Localization, Optogenetics...window) A collection of neurological mouse models including complex and monogenic diseases. Alzforum (Link...

... Enhanced colors, including PSmOrange, iRFP Gradia Lab Mammalian Plasmids - Includes tagging with mCherry...System Plasmids Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral...mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many N- and C-terminal fluorescent proteins Insect...Xenopus, and C. elegans Hamdoun Lab Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP.... elegans expression Yeast Thorn Lab Vectors - Includes tagging with a wide variety of colors, some specifically...yeast Lindquist Lab Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria...Bacteria Gradia Lab Bacterial Vectors - Includes tagging with mCherry, mOrange, mCerulean pET GFP - C-terminal...

...viewing on YouTube . Abstract The prokaryotic clustered regularly interspaced short palindromic repeats...each guide and its associated reverse complement including additional nucleotides for cloning and expression...48138) or pX459 (Addgene plasmid ID 48139), which include GFP and puromycin as selectable markers, respectively...promoter of the pX330 vector is enhanced by the inclusion of a G nucleotide after the CACC sequence. Add...instructions for adherent cells have also been included. Ensure there are 2 x 10 6 cells per CRISPR pair...obtain approximately 30 cells per 96-well plate. Include 100 μl per well of cell culture media. For the ... (2015). PubMed For the complete publication, including representative results and discussion, download...

...is all-inclusive of several important steps: MTA facilitation for the transfer plasmid, including legal...all-in-one pricing for viral vector preps, which includes MTA facilitation, DNA amplification, and high-...take approximately 6–8 weeks. Additional steps, including request approval, your institution’s approval ...produce high-quality viral vector preps at Addgene, including purification, titration, and quality control measures...information to help you every step of the way, including ordering, handling materials, using them in the...

... these viral vectors undergo quality control, including AAV titration by ddPCR, in vitro and (when possible...Mar;17(3):463-71. PMID: 19066593 Other citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated...;19(2):293-301. PMID: 21045809 Other citations include: Chen, et al. 2014. Reprogramming adipose tissue-derived...Sep;26(3):165-76. PMID: 26390090 Other citations include: Scalabrino, et al. 2015. Intravitreal delivery...90(8):4215-4231. PMID: 26865709 Other citations include: Kanaan, et al. 2017. Rationally Engineered AAV...Oct 19;6:35495. PMID: 27759036 Other citations include: Ling, et al. 2016. High-Efficiency Transduction...

...2) to completely unrelated molecules. Examples include: scFv - Single-chain fragment variables (scFvs)... used to quantify relative amounts of protein. Includes: Western blot ELISA Capture - used to isolate ...isolate specific proteins or protein complexes. Includes: Immunoprecipitation (IP) Chromatin immunoprecipitation...Visualization - used to visualize protein location. Includes: Immunofluorescence (IF) Immunohistochemistry ...identify, analyze, capture, and sort single cells. Includes: Flow cytometry Read on to learn more about the...the applications common to each category, including an outline of the basic steps needed for each application...run. The standard curve should contain a range inclusive of all expected protein concentrates in the sample...

...better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen, energy levels, and...processes. mTORC1 also blocks catabolic processes including autophagy and lysosome biogenesis. mTORC2 does...nutrients, but it is activated by growth factors, including insulin. mTORC2 influences cell survival and cytoskeletal...correlates with poor prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1...for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules and energy...

...that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins...collections or kits are described below and not included in this table. For a more complete list of plasmids...CRISPR technology. Addgene's worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones,...plasmids used to generate transgenic nematodes, including C. elegans and C. briggsae. Other related miniMos...Lab. A set of vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans optimized...

...expression in mammalian systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction...mRNAs. Both 5’ and 3’ Xenopus β-globin UTRs are included in the vector to enhance expression of the message...Golden Gate protocol. Both plasmids 47388 and 47389 include a Flag epitope tag and an SV40 NLS at the N terminus...mammalian cells. In addition, a T7 promoter was included for generating TALEN mRNAs through in vitro transcription...

... protein contains several functional domains, including: the N - t erminal D omain (NTD) the M ethyl B...omain (CTD) The most common missense mutations cluster in the MBD and NID demonstrating the importance...in a new window) JAX Mice for mouse models that include MECP2 null alleles or common mutations that recapitulate...window) Coriell Institute - cell line repository, including patient-derived iPSCs (Link opens in a new window...BrainSpan Atlas of the Developing Human Brain - includes developmental transcriptome information, prenatal...

...and pooled libraries. Viral preparation options include lentivirus and AAV. ...community on various aspects of viral vectors, including troubleshooting advice and explanations of viral...DNA Each viral vector service request will also include virus-associated DNA, which is a sample of the ...the Material Transfer Agreement (MTA) and its inclusion is what gives you permission to use the plasmid...

...mammalian systems. Other luciferases in our collection include Gaussia luciferase (Gluc) and the red and green...Split-Luciferase and Protein-Protein Interaction Assays Cluc-FKBP , FRB-Nluc : Split Firefly luciferase to assess...escape of cell-penetrating peptides , and more. nLuc/cLuc for split Firefly Luciferase complementation in ...cloning. Use with 174051 Alberto Macho 174051 pGWB-cLUC Firefly Creation of C-terminal Fifrefly luciferase...Multiple Multiple Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-...

...your gene of interest. Available modifications include histone acetylation by p300, histone demethylation...Publication RNA Knockdown Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA...Publication RNA Editing Type VI CRISPR systems, including the enzymes Cas13a/C2c2 and Cas13b, target RNA...genomic sequence - potential target locations can include both unique and repetitive regions. Fluorophores...

...plasmids that combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression ... approaches combine multiplexed recombinases, including Cre, Flp, and VCre to enable viral expression ... define cellular sub-populations of interest, including dual-transgenic recombinase-expressing mouse lines...the INTRSECT configuration have been developed, including additional fluorophores, excitatory and inhibitory...

... cleavage site. Includes sites for LIC cloning, and a "stuffer" fragment that includes the SacB gene, ...pZE31ms2 25860 MS2 coat protein binding sites Philippe Cluzel RNA localization: Contains a 2x array of the binding...DsRed-ms2x2 26248 MS2 coat protein binding sites Philippe Cluzel RNA localization: Contains a 2x array of the binding...

... of Cre recombinase plasmids. Special features include: Inducible Cre: These constructs require the addition...inducible Cre-GFP fusion, metallothionein MT-I region including the polyadenylation site and several introns tet-hCMV...developing limb mouse Prx1 Bacterial Tabin 14796 pClusterin-ERT2CreERT2 ERT2-Cre-ERT2 - Tamoxifen inducible...inducible. Expressed in Muller glia in the retina. Clusterin Mammalian Cepko 14797 pCAG-CreERT2 Cre-ERT2 - ...repress gene expression. Common construct types include: Cre-dependent Gene Expression: Placing a stop ...

...For each antibody, we provide important details including the protein and plasmid sequence, the antigen ...Applications section of our product pages. We also include negative results when an antibody’s use is not ...larger Antibody Plasmid Collection , which also includes other types of affinity reagents such as scFvs...

...promoter. For information about each catalog item, including the titer and quality control, visit the material... to Cart *Each viral service request will also include virus associated DNA (vdna), which is a sample ...