...pathogens including organisms such as Staphylococcus aureus or Vibrio cholerae . BSL-2 includes all of the...sharps container. Sharps include anything that can pierce your skin, including, but not limited to broken...experiment, make sure your workspace is clean and uncluttered. Disinfect your workspace with appropriate materials... all areas containing biohazardous materials, including biohazardous waste. Dispose of waste that may ...followed in addition to BSL-2 guidelines below, including PPE protocols . Working in a BSL-2 laboratory ...biohazardous waste containers that can be autoclaved. Conclusion Although simple, following appropriate BSL-1 ...

...and samples in duplicate at least If possible, include an AAV reference sample of known titer. The reference...expected titer of the samples being tested. Always include a No Template Control (NTC), i.e master mix + water... of each sample in duplicate. Do not forget to include a no template control ( NTC = master mix + water...dilution is appropriate). Quality of duplicates: Exclude duplicates from analyses if there is more than ...

...Other methods of generating a monoclonal cell line include single-cell sorting (Link opens in a new window... protocol will vary based on several factors, including the growth properties of the cell lines, the effects...cell pool by trypsinization and break up any cell clumps into individual cells by passing several times ...Wild-type A549 cells do not express Cas9 and are included as a control (WT). Vinculin is a loading control...

... it includes labs that work with agents associated with diseases in healthy humans. BSL-2 includes all...goggles in addition to the BSL-1 requirements. Conclusion Although simple, following appropriate PPE protocol...

...In this case, we will include the free nucleotide dGTP in the reaction (exclude all other nucleotides ...chew back" reaction, in which a single dNTP is included to stop the exonuclease function of the enzyme...

...used when the part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short...stitching” primers and the amounts of primers to include in the Gibson reaction are different than with ...window) sells DNA Assembly kits and master mixes, including NEBuilder HiFi (Link opens in a new window) and...

... purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ...the bacteria to lyse, releasing their contents, including plasmid DNA, into solution. Add a renaturing solution...step 6 to eliminate RNA contamination. This is included in the resuspension buffer of most kits. Reference...

... a certain position within the primer, one can include more than one nucleotide at that position termed...have complementary regions Note: If you will be including a restriction site at the 5’ end of your primer...

...Protocol for creating a glycerol stock, including additional tips for long-term storage....the appropriate temperature. Growth conditions, including copy number and growth temperature, can be found...

...Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

...to consider when selecting sequencing primers, including: Is the primer unique? Make sure the primer only...your plasmid, please email [email protected] and include: The sequencing result The original trace file ...

... You need to do this because you don’t want to include the weight of the weighing boat or paper into your...paper in the biohazard solid waste container. Conclusion Appropriately weighing reagents is important ...

...A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and...some of the key strategies of aseptic technique, including personal protective equipment, setting up a clean...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... is both functioning as expected and specific, include a positive control sample that you know expresses...

...protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

...cloning by restriction enzyme digest (subcloning), including design and experimental procedures....Design (Choosing enzymes) Many DNA analysis tools, including Addgene’s Sequence Analyzer , allow you to identify...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency...handling, use proper personal protective equipment including laboratory coat, laboratory goggles, and gloves...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... both functioning as expected and is specific, include a positive control sample that you know expresses...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... of the standard curve you will need to either include higher concentrations in the standard curve or ...

...without a kit. The general steps of this protocol include homogenization/lysis of cells or tissues, extraction...For the precipitation step, two options are also included: using Isopropanol or Lithium Chloride. This protocol...