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We narrowed to 593 results for: ELP

Showing: 181 - 200 of 593 results

  1. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...deficient in E1, E2, E3, and E4. Gutless Ad vectors (helper-dependent (HD-Ad), or high-capacity (HC-Ad__ are...packaging signal in cis. Therefore, co-infection with a helper adenovirus is required to provide the viral proteins... production is more complex and the presence of helper virus contaminants have slowed the use of this ...

  2. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...modified organisms. Since Addgene is very proud to help scientists share plasmids across the globe, we’re...generate far more legible labels than your hand and help keep things more organized. Cap tubes with a micronic... it isn’t electronic but it is one of the most helpful machines I have used in the lab. It’s especially...

  3. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...only a great form of scientific outreach, it also helps you to hone your writing skills and make a name ...Science Writers Association, EUSJA or ABSW for helpful tips and job offers. If you live in the UK, join...taking a media or journalism course. Courses can help you gain work experience and build your network,...

  4. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    Published
    Aug. 1, 2023, 1:15 p.m.
    ...assembly design (DAD) to build out three tools to help researchers design high-fidelity Golden Gate Assemblies...accomplished with Golden Gate Assembly—even when getting help from DAD. The 35-piece assembly mentioned above ...deposited by NEB – and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R...

  5. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...interest — for example, AAV8 for kidney cells — can help improve infection rates in your experiment. ... screen to a high degree. This is particularly helpful when investigating tissue-specific diseases, as...over lentivirus in many mouse tissues. This will help improve your selection pool for downstream NGS and...

  6. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...decrease the compensation value. If neither of these helps shift your population into the correct spot, you...markers can be challenging! But we hope this guide can help you get started. Remember, you can always change...finalize your compensation values later. This can be helpful as you're learning! Ashlyn has a research background...

  7. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...Where is the CRISPR community?  Addgene exists to help researchers share useful tools, and as such, we’...challenging. Addgene’s free educational resources help scientists navigate that learning curve. As of 2023...Genome Edit has 76,011 views.  Has all of this work helped scientists use CRISPR technology? We think so —...

  8. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    Published
    Sept. 12, 2017, 1:44 p.m.
    ...addition to human error, fragments of DNA from the helper plasmids or the cell genome can be packaged inside...genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., ...to discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality...

  9. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ... bullet points for what you would like to say to help build your presentation. For example, when thinking... fundamental takeaway. Writing these points will help you remember what to say and provide your audience...Having them reframe or elaborate their thought will help you to provide an on-topic answer by creating time...

  10. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...website for any relevant hashtags and handles to help you find other scientists attending. Reach out if...the conference hashtag to set one up. It’s also helpful to write your Twitter handle on your nametag for...are not looking for a job - those connections can help you later on. Update your LinkedIn and your resume...

  11. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...Alignment Search Tool (BLAST) provided by NCBI. BLAST helps us compare the sequencing results of the plasmids...differences in the blastn programs, check out the BLAST Help webpage. Optimizing blastx searches Once you have... on our suggestions? Share your thoughts here to help other labs speed up their plasmid and cloning verification...

  12. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ... recruit parents from any background, it really helps if activities are run by parents who can answer ...scientist. I think that establishing this history helped demonstrate that a) I was a real person who used... it worked. I came up with an analogy that would help them grasp some of the key concepts (Fig. 2). One...

  13. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...recurring meeting amongst scientists that aims to help stay current on new and emerging work by collaboratively...unable to attend for scheduling conflicts. This helped us find a time that accommodates more people. The...(multiple choice/binary) and open questions is helpful to ensure acquisition of the essential data and...

  14. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...productive and happy employees. The teambuilding culture helps us build relationships and thereby work better together.I...had in an orderly and timely fashion. 9. Ask for help My grandma used the old adage “many hands make light... lot of set up or cleanup, ask for volunteers to help out. Delegate- it will save you time and sanity!...

  15. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ... to submit an updated protocol? Just email us at help@addgene.org and we will be happy to upload your ...every lab's needs. Plus there are the apps - many to help you access your protocols at the bench, keep your...protocols show up on the related plasmid pages and can help scientists use these plasmids more successfully....

  16. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ... can help you hop, skip, and jump between all kingdoms of life. A bit of careful design will help make...include viral oris to drive amplification, which helps increase cellular uptake, but this approach requires...

  17. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...looking to see if there are any resources that can help with the transition.” The lab-to-desk-job culture...formal training regime and the right set of tools to help you do your job as well as possible. It’s in their... is an amazing thing to be a part of and really helps me get behind a project or initiative.” If you think...

  18. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...These demo details help us see the problem from the user's perspective which in turn helps us decide how to...software design choices, resulting in software that helps our users focus on the fun stuff, accelerating research...

  19. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...GoogleScholar, Academia, ORCID, and MyScienceWork all help us share research. Open access journals also provide... by Scott Spicer at the University of Minnesota helps illustrate this: “In terms of total view count and...are some resources: ImpactStory, a website that helps researchers go beyond citation rates to measure ...

  20. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    Published
    Oct. 25, 2016, 2:30 p.m.
    ...At Future of Research, our volunteers have been helping to gather information for our FLSA and postdocs... us if you have information that you think would help our resource. In the medium term, it is unclear ...in advocating for and with junior scientists, to help them navigate the scientific enterprise and promote...
Showing: 181 - 200 of 593 results