... CHIME and new X-CHIME systems, developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide... scenarios. X-CHIME We recently developed and deposited four new systems to fill this gap. To enable context-specific...Figure from LaFleur, et al. 2024.   The X-CHIME deposit also includes pXPR_071, a plasmid that leads to...

...performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, including many gRNA-containing...derived from scientists’ submissions, either their deposited gRNA plasmids or from submission of a spreadsheet...gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently have data...

... successful, internationally renowned plasmid repository that it is today. Although I wish I could take... attributed to the entire Addgene family; the depositors and requesting scientists, all of the Addgene... It was great to see the faces of some of our depositing scientists who weren’t able to join us in person...

...and a domain needed for DNA unwinding. They’ve deposited plasmids for active and inactive CasX for E. coli...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...bailii) and B3 (from Zygosaccharomyces bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP...cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested for excision efficiency...cross-reactivity; however, the other recombinase/reporter pairs were inactive, even at very high recombinase...

...Davidson's lab to coordinate the impressively big deposit. “He has every fluorescent protein under the sun...stage IV lung cancer that has now prompted him to deposit the tools he and his team created with the research...team who have made this large, important plasmid deposit possible.   ...

...background.” Just last month in Scientific Reports, Verkhusha’s team reported another advance in the application.... Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

...bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  ...double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this...challenging problem than repression, scientists have deposited several plasmid tools that can be used in this...microbiome Beyond the human gut, the Barrick lab has deposited plasmids that can be used to engineer the bee ...

...positive or more negative.  Neuronal stimulation: Depolarization When the inside of the neuron becomes positive...positive over a certain threshold, it becomes depolarized and will fire a signal. The non-specific cation...both cause ion movement that results in cell depolarization. They therefore function as a neuron “on switch...

...viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular cytotoxicity...., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen and ...opsonization. In antibody-dependent complement deposition (ADCD), opsonization of IgG and IgM antibodies...

... the most frequently requested vectors in our repository and will discuss some of the features you may...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...

... development of several fluorescent autophagy reporters now allows for the measurement of autophagic flux... al. "Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...using a single fluorescent biosensor." Scientific reports 5 (2015): 12397. PubMed PMID: 26215030. PubMed ...

...plasmids and to receive important coronavirus-related deposits. Our valiant lab and shipping staff agreed to ...to come in and start getting plasmid deposits into the collection and to start shipping. They created the...also moved fast to ensure that the majority of deposited materials to study SARS-CoV-2 would be fully open...

...the DNA-binding domain of AraC using a mCherry reporter downstream of a PBAD promoter. While none of the...contains BLADE, the PBAD promoter, and a mCherry reporter downstream of the PBAD promoter. To use this plasmid...shared with Addgene. You can find all the plasmids deposited from Di Ventura's lab, including the popular light-inducible...

... cells and activate expression of a luciferase reporter. CPP was not required for protein entry into cells...primary cell types using Cre and various loxP reporters. In both mouse and human embryonic stem cells,...Plasmids @Addgene: iTOP-tested proteins and reporters Previous Protein Delivery Techniques: Introduction...

...actively require these skills, including: Science Reporter Scientific Sales Epidemiologist Scientific Recruitment... actively use these skills include: Science Reporter Scientific Illustrator Research and Development...desirable in a range of roles, including: Science Reporter Technical Writer Statistician Bioinformatician...

...specific author guidelines for reporting your materials. If you deposit a plasmid with Addgene, your plasmid...to ensure that materials available through our repository are verified and identifiable to save researchers...

...control process alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took the joint...the scientists I've already mentioned, Addgene depositors Marius Wernig, Kristin Baldwin, Kathrin Plath...spoken at this meeting. In fact, we have so many depositors from the stem cell community that they have their...

...not used for general plasmid selection. Instead, reporter genes are often employed to easily monitor transfection...expression levels in the cells. Ideally, the chosen reporter is unique to the cell, is expressed from the plasmid...assess transfection success. GFP is often used as a reporter and we will be covering this ever-versatile fluorophore...

...usually spread unidirectionally, there are some reports of bi-directionality. For predominately anterograde...cleft is usually severely impaired, and only few reports exist about dye molecules that are transported ...2008; Watakabe et al., 2015). There are several reports of both retrograde and anterograde intracellular...replication like RABV or HSV. There are, however, a few reports of trans-synaptic spreading of some AAVs, specifically...