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We narrowed to 867 results for: FER

Showing: 181 - 200 of 867 results

  1. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...Addgene now offers packaging plasmids for AAV6 and AAV11! Why these serotypes? AAV6 offers broad tissue... 1). See the paper for more details on small differences between mGold2s and mGold2t depending on the ...mGold2t for your next experiments and see the difference! Find mGold2 plasmids here! Lee, J., Lai, S....Created with BioRender.com. Steven Jacobsen and Jennifer Doudna’s labs engineered the tobacco rattle virus...libraries are available for tracking cell lineage, different applications require careful selection or design...Clonal Kinetics (STRACK) to study stem cell differentiation and cancer fates (Singh et al., 2025). Key ...stem cells and track clonal dynamics during differentiation after they introduced mutations that drive ...

  2. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...several different markers, it can also be used to run multiplex assays, allowing the reference sequence...primer  Molecular Biology Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll...each cycle. The raw data is then normalized to a reference sequence present in the same amount in all samples...curve. For CNV assays, it is recommended to use a reference sample with two copies of the gene (Ma, 2014.)...probe set for both the gene of interest and the reference sequence. At the end of the run, each droplet ...positive droplets for the gene of interest and reference gene, copy number can be determined through concentration...that matches your needs. Happy quantifying!  References and Resources More Resources on the Addgene blog...

  3. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ... are composed of many different gRNA-containing plasmids, each with a different genomic target. The goal...expressing cells. Pooled lentiviral CRISPR libraries (referred to simply as “CRISPR libraries”) consist of a ...heterogeneous population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene within.... Pooled gRNAs are then cloned into lentiviral transfer vectors to create the CRISPR library. CRISPR libraries...cell or set of cells containing a mutation in a different gene. Libraries may be available in a 1-plasmid... be used in drug screens to identify genes that confer drug-resistance. Mutant cells are treated with ...gRNAs that are “enriched” correspond to genes that confer drug resistance when mutated. Findings from this...

  4. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...Uniform Biological Material Transfer Agreement (UBMTA) for its material transfers. The UBMTA was drafted by... has time for paperwork? Here’s why a Material Transfer Agreement (MTA) matters: Science thrives on collaboration...universities and institutions require them for material transfers. When Addgene entered the plasmid sharing space...or choose to auto-approve MTAs. Whatever your preference, we are here to make it fast and easy. Fast...receiving institution. Therefore, many technology transfer officers are familiar with the UBMTA and are happy....org or +1 617-225-9000, or check out our MTA reference pages. So go ahead and use Addgene plasmids ...  material transfer agreement...

  5. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...about your different experiences in research labs. What are some similarities and differences you have ...found that research institutes in different places have very different cultures. In Basel, I felt that ...recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress PCR amplification...join the Advisory Board of Addgene, I accepted the offer without any hesitation. I hope I will be able to...world? Fujii: Fortunately, I was able to work in different parts of the world in my early research career...technologies, hopefully they'll be completely different from what we are now doing in my lab then when...to Alerts" link in the Addgene Alerts box. References Identification of Proteins Associated with an...

  6. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...opposite direction to fluorescence collection is referred to as “Epi” illumination. Therefore these are ...incorporate optical sectioning. Optical sectioning refers to the ability to extract light from a single, ...excitation light path. Each hole corresponds to a different location in the sample. As more than one hole ...between the glass coverslip and a sample in aqueous buffer it is completely reflected. This reflection occurs...index mismatch between the glass and the water-like buffer the sample is immersed in. Although the excitation...one of four categories that are each suited to different types of modern day fluorescence microscopes (...means that the images will appear sharp without interference from blurry out-of-focus light. The most difficult...

  7. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...many countries, import and export regulations, differing customs processes, and the distances the packages...distributes to per year, 2005-2023. We identified and offered a number of solutions that increased accessibility...each courier we contracted with so we can always offer the fastest shipping. However, there were issues...specific to that country. ‌For example, when we began offering temperature-sensitive materials, we noticed a ... backbones, lentiviral packaging systems, and luciferase expression. On individual material pages, we ...educational resources for each type of resource we offer.  In 2013, we launched the Addgene blog for topics...particular topic into beautifully designed and easy-to-reference eBooks. Figure 3: Global readership of the Addgene...

  8. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ...strains of mice. This suggests that a small genetic difference between these two mice might drive their divergent...took a broad approach and searched for genetic difference between the 36 mouse strains that are part of...permissive phenotype, but only one, Ly6a, showed differential protein and RNA expression between the brains...are not reproduced when the vector is used in different mouse strains. The in vivo selection for PHP.B....eB viral preps are available from Addgene. References Holmes, Christina, and William L. Stanford. "...performed in non-human primates, check out these two references: Dayton, Robert D., Mychal S. Grames, and Ronald...Ronald L. Klein. "More expansive gene transfer to the rat CNS: AAV PHP. EB vector dose–response and comparison...

  9. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...optimized for dual-AAV systems. These variants use different RT enzymes and performed large edits with similar...types. DIRECTED is compatible with fusogens from different viral families and can be used to target multiple... to reduce negative side effects. This platform offers a promising approach for experimental therapies... enzymes, TurboID and APEX2, are localized to different cellular compartments. They showed APEX2 can use...uses sequential labeling by TurboID and APEX2 at different cellular locations. C) Enrichment of dual-labeled...stably or inducibly express tagged iPAK4 from different promoters, specific time points appear as fluorescent...bands of color with HaloTag-iPAK4 by applying different HaloTag dyes and showed they could record transcriptional...

  10. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...acquired drug resistance are referred to as methods of ‘horizontal gene transfer’. Resistance can also arise...you! Many thanks to our Guest Blogger, Jennifer Rivers! Jennifer Rivers ScM, MLS(ASCP)CM is a research ...This post was contributed by guest blogger, Jennifer Rivers, a research microbiologist specializing in...multi-drug resistance are resistant to three or more different classes, or types, of drugs used to treat them...either through random mutation or horizontal gene transfer, means to defend themselves from these naturally...resistance can arise, and has arisen, for many different reasons. Exposure to antibacterial drugs provides...science communication, and public health.     References 1. O'Neill, J. "Tackling drug-resistant infections...

  11. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ...patients suffer any less acutely.  The Rare Genomics Institute works to help those who suffer from rare...in diabetes and lymphoma research or the many different ways you can improve your heart health. These ...disease research and provide hope to patients suffering from these devastating afflictions. Elia Di Schiavi...abdomen and affects roughly 20% of mesothelioma sufferers. This disease is less widely understood and often...for peritoneal mesothelioma and thereby relieve suffering from this little-known disease. You can find more...Nabavi and will continue to work to help those suffering from rare diseases. These underrepresented individuals...rare disease research funding opportunities. References 1. Jones, C., et al. "PP09. 1–2352: Systematic...

  12. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...through the proliferation of specific, and not all, Langerhans cells, to create foci of proliferating cells....construct containing fluorescent proteins (XFP) of different colors to label an organ or entire organism. Because...more refined versions have been developed, which differ in the number, stability, and brightness of the...localization sequence, allowing it to localize to a different area of the cell than CFP and YFP. The most recent...could recombine to activate the expression of a different fluorescent protein. Therefore, each cell could...become a powerful tool for understanding cell proliferation. Inducible XFP expression in the Ubow mouse ...can be combined with new imaging techniques.  References Cai, Dawen, et al. "Improved tools for the Brainbow...

  13. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...through a tiny pore by measuring differences in ion flow caused by the different electrochemical properties ...fragmented into smaller pieces, run on a gel, and transferred to a membrane for analysis through hybridization...standard PCR. This allows the researcher to compare different DNA sequences to see which is more abundant in...content by comparing the fragments created by different restriction enzyme digests. Incubating a DNA sample...restriction mapping (Figure 1). Though some labs may prefer to send DNA directly for sequencing (more on that...incorporation of a ddNTP. Researchers perform four different reactions, one for each radiolabelled ddNTP, and...machines (9). Today, similar technologies, sometimes referred to as sequence DNA as it synthesized by electronically...

  14. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...understand genetic cytoplasmic factors that could be transferred between cells. At the time, these extranuclear...episome seemed appropriate, especially since the Fertility, or F-factor discovered by Ester Lederberg in ...-factors. Like F-factors, R-factors could be transferred between bacteria via cell-to-cell contact; however... the same. Over the next few years genes from different bacterial (and eventually mammalian) species were...ubiquitous tool that can be diversely applied to many different experiments. Addgene was founded in order to store... keeping with our goal of creating a one-stop reference guide for plasmids we've updated and added new...: 24043817. PubMed Central PMCID: PMC3785787. References: Lederberg, Joshua. "Cell Genetics and Hereditary...

  15. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...specific problem. Every species is so different – an awake monkey is different from a cell in a dish. I always...of life. Our most recent paper in Nature Methods offers the first true two-color pair. We'll be sending...want to activate two sets of neurons and see how different parts of the brain or the cells within them work...set of neurons, maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other...Boyden: I think because neuroscience has so many different goals: to understand how a mouse changes emotions...find ways to make these tools more selective for different ions. Right now, we can modulate electricity. ... click the link in the Addgene Alerts box. References: Independent Optical Excitation of Distinct Neural...

  16. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...deposits to Addgene.  Insomuch as different journals represent different research areas, target readers,... would also be great to know how much of that difference in citations is caused by depositing a plasmid...wouldn’t be causal. To control for a number of differences between those papers with Addgene plasmids and..., so our estimates now take into account any differences in citations due to the journal of publication... the same journals! So, is all this remaining difference caused by depositing the related plasmids with...it is still suggestive that we see such a big difference in citations after controlling for journal. And...Visit his website at: http://www.neil-t.com/ .   References 1. Cong, Le, et al. "Multiplex genome engineering...

  17. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...research program (think a subtle difference in mouse chow, a different water filtration system, environmental...outline and refer back to the original paper that everyone in your lab has been referencing for the last...original researchers actually did. Admittedly, the differences are often very subtle, and it can take a lot ...and it was just that they were doing something differently. Communication comes in many forms—from transparency...other publishers and organizations have various different versions of checklists and guidelines to help ...rewarding good scientific citizenship in all its different forms as part of graduation, hiring, funding, ...

  18. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to various Cas9 variants, ...either MS2, PP7, or boxB) that each recruits a different fluorescent protein (BFP, GFP, or RFP) to the ...green, or red readout. But gRNAs containing two different hairpins will produce a secondary color (yellow...allows for simultaneous visualization of sites on different chromosomes in living cells, the system requires...they chose an intergenic DNA region (IDR3) as a reference loci to measure inter-locus distances of loci ...sgRNA-Sirius-4X(MS2-PP7) showing recruitment of different fluorescent proteins. (B) CRISPR-Sirius used to...try. Find CRISPR-Sirius plasmids at Addgene. References Ma, Hanhui et al. “Multicolor CRISPR labeling...

  19. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...not exert force by dynamically rearranging into different polymerization states. Instead, R body monomers...natural context or 3) be modified to extend at different pH levels. Domesticating R bodies We have shown...that R bodies can function to break membranes of different organisms. And finally, we demonstrated that R... can be “tuned” with mutagenesis to extend at different pH values. Using a screen that takes advantage... fact that coiled R-bodies sediment in certain buffers but remain soluble when extended (see figure to...region produced R-bodies that could extend at different pHs. Plasmids expressing expressing both wild ...School. Follow her on Twitter @jessicapolka.     References 1. Kanabrocki, J. A., Quackenbush, R. L. & Pond...

  20. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ... labs (see table below) have different PAM requirements. These different PAM requirements allow researchers...discovered all the time and the requirements of these different systems can vary (for example, xCas9 is a variant...neither bind nor cut the target. Cas9 homologs from different organisms or Cas9 mutants developed in a variety...researchers to target many different genomic loci. 3. Cleave target DNA resulting in a double-strand break...). Throughout the remainder of this post, we’ll refer to this RNA complex as the “gRNA”. The gRNA contains...choice (see tips for designing a gRNA) and their prefered Cas9 variant (with the appropriate PAM sequence...sequence.   Now that you know about all the different components of CRISPR, It's time to start thinking...
Showing: 181 - 200 of 867 results