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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 253 results for: IRES

Showing: 181 - 200 of 253 results

  2. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...part of the Class 1 CRISPR family, which means it requires multiple Cas effectors to carry out genomic editing...

  4. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...conditional knockout. This experimental design requires two rounds of recombination – the first removes...

  6. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...Potential PIs Even if you’re applying to a school that requires its students to do rotations in different labs...

  9. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...many FPs (particularly those derived from GFP) requires oxygen. Therefore, these FPs cannot be used in...

  10. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...sense of what AdVs are about – and we hope that it inspires further use of existing vectors, as well as research...

  11. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...of - and ExM is no exception. First, expansion requires fixed samples. This limitation may be the both...

  12. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...expressed in bacteria, which means their production requires animal sacrifice and precludes genetic engineering...

  15. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...

  16. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...PMC4466736. 5. Ando, Hiroki, Sebastien Lemire, Diana P. Pires, and Timothy K. Lu. “Engineering Modular Viral Scaffolds...

  18. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification...

  20. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...strand is synthesized (Figure 1). Sanger sequencing requires four separate reactions, where a fraction of one...
Showing: 181 - 200 of 253 results