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We narrowed to 895 results for: Ide

Showing: 181 - 200 of 895 results

  1. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...innovative ideas in all facets of the entrepreneurial process in order to develop those ideas into commercial...excellent people with mediocre ideas than mediocre people with excellent ideas. I am lucky to have launched...self-knowledge and an unwavering devotion to seeing your ideas become something tangible.  When you think of entrepreneurship...company, you need to determine whether or not your idea is viable.  Go to experts you trust and pitch what... received from our network.  We started with the idea of a biotechnology investment fund that would apply...our early stage.  After each pitch we made, we considered the feedback and adjusted our business model ...You’ll find that no matter how well you know your idea, you will rarely succeed in launching a company ...

  2. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...Trends: Most tools provide tabular HTML formatted output. Surprisingly only 2 tools provide FASTA output. ...success to technical expertise, thoughtful single guide RNA (sgRNA) design, and the abnormally low GC content...OMICtools. It must be confusing for a newcomer to decide on the right software to use. With so much choice...sgRNA design tools The goal of this post is to provide some insight into available CRISPR software tools...qualities of what determines good sgRNA design. Incidentally, the first software for sgRNA design (by Hsu...scoring algorithms and parameters. This tapestry of ideas is what makes choosing the right tool confusing ...functions are not entirely necessary but should be considered extremely useful, depending on design goals. ...

  3. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...resistance to pyrazinamide (PZA) (a drug commonly used to treat tuberculosis) and in identifying new drug ...wild lettuce. Aside from their use as food, infusions of L. taraxacifolia leaves are widely used to treat...Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and plasmids to...specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to these researchers. In the third round...control. In her current research, Sheen aims to identify new genes related to PZA-resistance by using different...using CRISPR on our blog). Her research should provide us with a better understanding of PZA action and...treat several diseases. Her goal is to identify and isolate pure plant compounds with antidiabetic properties...

  4. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...few minutes. The host could also set aside Breakout Rooms to divide the audience into small sessions and...To date, aside from doing science, he has assisted many researchers in gaining the confidence to launch... at the lab meeting, the team can discuss those ideas and build from those.   Figure 1: Virtual...focus on. Use collaborative cloud apps It is a good idea to use collaborative cloud tools such as Google ... and Sheets during Zoom lab meetings to chip in ideas and take minutes that can be accessed by anyone ..., where everyone stands up, disappears from the video, and prepares a coffee, or simply moves away from...I believe this is a great functionality that is widely used and could bring back a taste of that casual...

  5. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...Cas9, for which efficiency varies widely across guide RNAs, each guide tested with CasRx yielded >80% knockdown...Cas13d proteins they identified have little sequence similarity to previously identified Cas13a-c orthologs... to tap the wealth of CRISPR possibilities. To identify Cas13d, both groups used very general bioinformatic...independently process their own CRISPR arrays into guide RNAs. crRNA cleavage is retained in dCas13d and ...its low immunogenicity. But these studies also identified other advantages, including Cas13d-specific regulatory...produced very similar cleavage products even when guides were tiled across a target RNA, indicating that...Previous CRISPR exon-skipping approaches used two guide RNAs to remove a given exon from the genome, and...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...ongoing Plasmids 101 series. Plasmids 101 will provide you with an overview of general molecular biology...plasmid with an insert? Blue-white selection is a widely used method to do just that! (To read about other...lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate, IPTG binds...x-gal (5-bromo-4-chloro-3-indolyl-β-D-galacto-pyranoside) into galactose and an insoluble blue pigment...encoding this section of amino acids (called the α-peptide) to a lacZΔM15-mutant bacterial cell in trans complements...engineered a multiple cloning site (MCS) into the α-peptide (represented as an orange wedge in the figure on... your DNA (red) is cloned into this MCS, the α-peptide gets interrupted as shown in cell A, and thus will...

  7. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...relationships outside your organization. The mentor in your own workplace is able to: Help you identify specific... and become an insider faster A mentor that works elsewhere is able to: Help identify the skills you... you meet, the more potential mentors you will identify. Finding potential mentors is one of the many ...done this with people I work with and people from outside my work for almost 20 years. It is good to be a... this recent blog 5 Strategies for Finding Your Ideal Mentor. One of the best was to notify your network...family and friends know you well and might have good ideas for who you should connect with. Listen to our podcast...you will need to make a change Provide perspective on core values at another company/department Give more...

  8. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. What you might not...this kit have been used to purify a variety of peptides including antibiotic efflux pumps and ankyrin ...followed by leader sequences (encoding small leader peptides) downstream of which you can clone your gene of...interest. Translation of the standardized leader peptide prevents secondary structures in the mRNA encoding...from resulting in the production of a downstream peptide, the transcription terminators in this series are...plasmids from this kit include the production of a suicide switch in cyanobacteria, synthetic inverters in...biotechnologically important microalgae: intrinsic suicide switch implementation in cyanobacterium Synechocystis...

  9. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...demographics and the information is often confidential (see image below). Slide of information gathered by Postdoctoral... this very idea that postdocs are acquiring training to become professors that is provided as an explanation...transferrable to other environments. Slide from Paula Stephan’s Talk (All slides can be found here). Importantly...information does not, however, prevent us from identifying problems with the structure of the academic research... research workforce, and we are beginning to identify potential solutions. Keynote speaker Paula Stephan...masters programs to prepare scientists for positions outside of academia Require that postdoc salaries be paid...coming months and welcome guest bloggers who can provide additional advice to trainees navigating the waters...

  10. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    Published
    May 11, 2021, 1:15 p.m.
    ... be identified using antibodies and immunohistochemistry techniques. Thus, these proteins provide a versatile...to the plasma membrane.   Our studies provided evidence that some fluorescent proteins can be, in ...facilitate its detection. Fluorescent proteins are most widely used tags because they emit endogenous fluorescence...versatile method to identify the expression of transgenes of interest. Furthermore, fluorophore variants...red fluorescent proteins) allow simultaneous identification of more than one protein of interest. However...were still cytoplasmic. Use of ‘small’ tags to identify proteins Given the limited expression of hM4Di‐mCherry...proteins when the fluorescence is not essential to identify the expression of the opsin or chemogenetic receptor...

  11. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ... BioID platform for identifying protein-protein interactions, but RaPID identifies RNA-protein interactions...ligase BirA* from BioID and allows a researcher to identify proteins that bind an RNA motif of interest in... like cross-linking immunoprecipitation (CLIP) identify RNA-protein interactions by cross-linking RNAs...using proteins as bait has limited utility for identifying novel RNA-binding proteins that interact with...RNA of interest. Current labeling methods are not ideal for in vivo work. BirA* requires 16-18 hours to ...Repository for Affinity Purification (CRAPome), to identify highly biotinylated background proteins reported... not well characterized RNA motifs. This bait identified a new interaction: binding of the RC2H1 protein...

  12. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ... Addgene's resident blog editor, I could take the needed steps to continue where the guide left off - ...molecules can bind to a wide variety of targets, including proteins, peptides, amino acids, drugs, metal...I love Addgene's new Antibody Guide and not just because I helped create it. But while antibodies, as...its thorns and every affinity reagent has its downsides. These reduced-in-size options often have reduced...situations where a suitable antibody has been identified but a smaller or less immunogenic version is ...light chain regions, connected by a short linker peptide. The lack of constant region makes them less immunogenic...proteins formed by two Fab fragments bound by short peptide linkers. They are bispecific, with a lot of flexibility...

  13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    Published
    Aug. 20, 2019, 12:31 p.m.
    ...experimental design considerations used in all behavioral studies. General considerations for behavioral ...should be avoided as this will also increase stress levels and affect behavior. A fact to consider: Rodents...behavioral testing As a general consideration for behavioral testing in a laboratory, a high level of experimental...fluorescent light bulbs, for example) that are outside the humans’ audible spectrum. The experimental ...gene expression in a specific brain area, avoiding side effects from such modification in other areas. An... particles are smaller and may be preferable if wider diffusion is required. On the other hand, lentiviral...targeted to a specific area. Some other technical considerations for stereotaxic injections are: Vector titer...

  14. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...go-to providers of materials needed for optogenetics. There are several important considerations with ...optogentics to answer a research question. The flip side of the optogenetics coin is materials science-based...dental cement (a similar procedure as implanting a guide cannula). A fiber optic patch cable is then connected...materials required for each [1, 2]. This protocol [3] provides, in exquisite detail, the various steps required...able to penetrate brain tissue a short distance so ideal implant placement should be about 0.4-0.5mm above...stimulation. The scatter effect is also an important consideration when preparing the implant itself, which brings...least 85% of the initial laser output. The same considerations apply to the polishing and preparation of the...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...with the guide RNA.   Non-target strand The DNA strand that does not base pair with the guide RNA. Despite...hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com. Although...ABEs are base transition editors. They adjust the side groups of DNA bases and leave the core ring structure...some cases, the same repair pathways that were considered problematic in the development of CBEs and ABEs...structure. In order to bind DNA, Cas9 requires (1) a guide RNA, (2) a complementary DNA sequence, and (3) a...strands called an R-loop (Figure 2). This allows the guide RNA to base pair with the DNA target strand, leaving...non-target strand unpaired and accessible on the outside of the enzyme. Figure 2: CRISPR R-loop. ...

  16. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...knockdown, or other factors, consider using one of the alternative, albeit less ideal, verification approaches...the results across runs. If the results vary considerably, then the high degree of variability in the ...replicates are distinct samples that are treated identically, used to assess naturally occurring random biological...assays have their own unique controls that must be considered (Burry 2011). Perhaps the most critical are those...wild-type and knockout samples are treated with the identical protocols that the experimental samples undergo...with its antigen or a small epitope-containing peptide to block the antigen-binding site. The sample is...detectable, it is likely due to binding interactions outside of the antigen-binding site.      Secondary antibody...

  17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...industry, nonprofits, and publishing. The livestream video from the event can be found below along with a description...reproducibility “crisis” on our hands and instead provide a brief conceptual framework for the causes of ...protocols and use one another’s reagents with the confidence that they aren’t simply doing an experiment wrong...Luckily, many organizations have been working to provide tools that make it easier to share in-depth information...working with a plasmid from Addgene, you can be confident that you’re using the actual tool that you ordered...research needs. Other companies like NEB and ATCC can provide you with additional validated reagents, and, with...Protocols.io, JoVe, and Bio-protocol all work to provide more descriptive and interactive protocols that...

  18. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...designed small RNA, or guide RNA (gRNA). The gRNA conventionally contains a 20-nucleotide sequence that is ...Activators: A Practical Guide Additional Resources on Addgene.org Check Out Our CRISPR Guide Pages Find Validated...nuclease-null—in order to effect different perturbations. Consider, for instance, the challenge of attempting to ...interacts with a distinct gRNA that allows users to decide which Cas9 protein is directed to which target ... truncated such that it exhibits 15 or fewer nucleotides of complementarity to a target site, Cas9’s DNA...truncated gRNAs Importantly, the use of truncated guides has largely been demonstrated to result in decreased.... An additional benefit of the use of truncated guides is that they can be delivered into systems that...

  19. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ... about my interests and my ideas all the time. Once, I had this crazy idea that I needed help with: wouldn... you make the most of the opportunities you’re provided throughout your PhD program.   Find people who.... They didn’t always know the best direction to guide me because they had no experience with my career... open mind and open ears. Everyone I talked to provided new data. Every new experience taught me something...troubleshooting.” Trying out new experiments and new ideas is nothing new to a scientist. Framing my efforts...efforts in these comfortable terms helped guide me through the process. As I mentioned, my graduate office...disappointed in the lack of participation, but I decided to focus on what the experience taught me. The ...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...as well as highlight some considerations for their use. Then, we’ll provide information about commonly...decision tree can help guide your choice on which systemic capsid to use. Factors to consider include the delivery...goes beyond such restrictions, you may want to consider a systemic capsid for your AAV.   Systemic capsids... definition of systemic capsids. After some consideration, we offer this definition: A systemic capsid... after intravenous administration, distributing widely throughout the organism to target large or diffuse...commonly used and emerging capsids to help you decide which ones may be suitable for your experiment. Like all...and/or its intermediate engineered precursor. Considering systemic capsids for your research? While systemic...
Showing: 181 - 200 of 895 results