We narrowed to 465 results for: MEA;

...cell needs to be fixed and the cell membrane permeabilized in order for the antibodies to reach their intracellular...colocalization experiments in a scientific and meaningful way it is important to choose the appropriate...

...suggest retransforming the plasmid into a strain meant for DNA purification. It sounds like extra work,...harvesting and miniprepping at various time points. Measuring the optical density of the culture can indicate...

...nontrivial propensity to noncovalently dimerize. That means that your POI fused to GFP or another fluorescent...whorl formation assay, are practical real world measures of dimerization potential that will determine ...

...vectors do not have to exclusively be used as a means of detection; they are useful almost anytime you...Glycine and Proline residues found on the C-terminus meaning the upstream cistron will have a few additional...

... a stable, easily purified protein with easily measured activity, MDH is particularly amenable to in-class...tag, and compared their specific activities by measuring NADH production.   CUREs are beginning to...

...Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of antigens (... size of its DNA cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot be delivered...

...going: If your PTA has a general meeting and a means to request funding for events, consider reaching...as coming from networking at the time, but that means it was the best kind of networking! Since nearly...

... us to transform raw genomic coordinates into meaningful experiments. Table 1: An emerging family of "...periodically use next-generation sequencing to measure the relative proportion of guide RNAs (gRNAs) across...

...other viruses that cause disease in humans such as measles, mumps, and rabies. Research on Ebola virus has... luciferase. Firefly luciferase can readily be measured to quantify (indirectly) transcription performed...

...frameshift or 21+ bp indel. This score is a useful measure to determine how many of the contributing indels...frameshift or 21+ bp indel. This score is a useful measure for those who are interested in understanding how...

... natural selection of sequences that evolve a measurable property of a protein. As a proof of concept,... allow for sensitive and high spatiotemporal measurements of norepinephrine. These norepinephrine sensors...

...complexes, wash away unbound protein, elute and measure the target. However, the two different approaches...the additional benefit of permanent linkage. This means that the primary antibody will not co-elute with...

... with one of these tools, it doesn’t necessarily mean the gene is going to express well. If you do get...PMC3565066. 7. Quax, Tessa EF, et al. "Codon bias as a means to fine-tune gene expression." Molecular cell 59.2...

...plant sciences “transient transformation” usual means that neither a stable cell line or plant is regenerated...GUS and are therefore good temporal reporters to measure promoter activity, for example. Useful positive...

...transient transfection, the vector remains episomal, meaning it stays separate from the genome. One option for...replicate during the course of your experiment, meaning you don’t have to worry about vector replication...

...you remember everything? Write it all down! And I mean everything! The bare minimum should be taking notes...invaluable. With a little thought you can build meaningful relationships in your new lab, learn the required...

...think “scientist.” But leaving the bench does not mean you’re not a scientist anymore. Being a scientist...their entire career. Yet even success in academia means you’ll eventually end up as a PI working what is...

...it’s difficult to get data out of the ELN. This means that reproducibility is limited in two ways – only...external file storage resources remain live. This means that someone who wants to query and attempt to reproduce...

... a result, mesothelioma is often misdiagnosed, meaning that by the time it has caught it has usually started...in the body. Such misdiagnosis and late discovery mean that most mesothelioma patients have an extremely...

...Share science, video usurps the written word as the means for communicating research. Short videos of 3 to... helps researchers go beyond citation rates to measure the impact of their contribution. It offers a 5...