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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 461 results for: MEA;

Showing: 181 - 200 of 461 results

  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...vectors do not have to exclusively be used as a means of detection; they are useful almost anytime you...Glycine and Proline residues found on the C-terminus meaning the upstream cistron will have a few additional...

  4. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of antigens (... size of its DNA cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot be delivered...

  5. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...going: If your PTA has a general meeting and a means to request funding for events, consider reaching...as coming from networking at the time, but that means it was the best kind of networking! Since nearly...

  6. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ... a stable, easily purified protein with easily measured activity, MDH is particularly amenable to in-class...tag, and compared their specific activities by measuring NADH production.   CUREs are beginning to...

  8. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...transient transfection, the vector remains episomal, meaning it stays separate from the genome. One option for...replicate during the course of your experiment, meaning you don’t have to worry about vector replication...

  9. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...frameshift or 21+ bp indel. This score is a useful measure to determine how many of the contributing indels...frameshift or 21+ bp indel. This score is a useful measure for those who are interested in understanding how...

  10. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...challenging. The fast paced nature of projects often means working long hours both at home and at the office...and educational parts of the job. Is consulting meant for PhDs? Healthcare consultants are usually trained...

  11. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...experiments carried out on low passage cells and this means you can regularly discard the cells you are working... actual flasks of cells in the incubator). This means any other scientist who wishes to use these cells...

  12. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...think “scientist.” But leaving the bench does not mean you’re not a scientist anymore. Being a scientist...their entire career. Yet even success in academia means you’ll eventually end up as a PI working what is...

  13. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...it’s difficult to get data out of the ELN. This means that reproducibility is limited in two ways – only...external file storage resources remain live. This means that someone who wants to query and attempt to reproduce...

  14. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... with one of these tools, it doesn’t necessarily mean the gene is going to express well. If you do get...PMC3565066. 7. Quax, Tessa EF, et al. "Codon bias as a means to fine-tune gene expression." Molecular cell 59.2...

  15. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ... a result, mesothelioma is often misdiagnosed, meaning that by the time it has caught it has usually started...in the body. Such misdiagnosis and late discovery mean that most mesothelioma patients have an extremely...

  16. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...Share science, video usurps the written word as the means for communicating research. Short videos of 3 to... helps researchers go beyond citation rates to measure the impact of their contribution. It offers a 5...

  17. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...plant sciences “transient transformation” usual means that neither a stable cell line or plant is regenerated...GUS and are therefore good temporal reporters to measure promoter activity, for example. Useful positive...

  18. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...you remember everything? Write it all down! And I mean everything! The bare minimum should be taking notes...invaluable. With a little thought you can build meaningful relationships in your new lab, learn the required...

  19. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...complexes, wash away unbound protein, elute and measure the target. However, the two different approaches...the additional benefit of permanent linkage. This means that the primary antibody will not co-elute with...

  20. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... natural selection of sequences that evolve a measurable property of a protein. As a proof of concept,... allow for sensitive and high spatiotemporal measurements of norepinephrine. These norepinephrine sensors...
Showing: 181 - 200 of 461 results