We narrowed to 516 results for: PROC
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TypeBlog PostPublishedMay 20, 2021, 1:15 p.m....doesn’t, and trying all over again. Here’s that process! Figure 1: The Design-Build-Test-Learn...gear, and walking everyone through the recording process was going to be time consuming and difficult. Finding...in the future, but we were finally refining the process down to a place I could be happy with it. Cycle...
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Plasmids 101: Biotinylation
TypeBlog PostPublishedNov. 15, 2018, 1:50 p.m....These biotin dependent enzymes are essential for processes such as gluconeogenesis, lipogenesis, amino acid...energy transduction. Biotinylation is the general process by which biotin is added to a protein or macromolecule...biotinylation occurs naturally in cells and is the process by which biotin is covalently bonded to a specific... -
Selecting Your Plasmid Purification Kit
TypeBlog PostPublishedMay 27, 2025, 1:15 p.m....that can arise during the plasmid purification process. One is the possibility of damaging the plasmid...carrying over contaminants from the plasmid prep process into the final plasmid eluate. Possible contaminants...contaminants can sneak in from almost any step of the process and include things like genomic DNA, RNA, proteins... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon...inefficiency? Use CRISPR to make it a selectable process! As NHEJ is ineffective in bacteria, CRISPR-induced...collection of organisms, their genes, and metabolic processes, occupying the gut. Many human diseases have connections...properties of CRISPR-Cas systems is base editing. This process is generally accomplished by fusing an enzyme capable... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog PostPublishedJune 13, 2019, 12:19 p.m....steps in the viral production and quality control process to improve both the yield and quality of the viral...scientists with the means to study biological processes on the cellular level instead of having to work... -
Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More
TypeBlog PostPublishedNov. 15, 2016, 3:30 p.m..... Listen to this episode for insights into the process of getting an MD PhD, the inside scoop on developing...family. 3:56 - 7:04 : Alex describes the MD PhD process and explains why he loved the first 2 years of ... -
Newly Updated AAV Data Hub!
TypeBlog PostPublishedFeb. 7, 2023, 2:15 p.m....experiments - and we've updated our submission process so you can easily add your data, too! Using...should!) submit your data to the Data Hub! The process is easy with our new submission form - and no worries... -
The Addgene Antibody Data Hub
TypeBlog PostPublishedSept. 12, 2024, 1:15 p.m....antibody worked in your application. The submission process is easy, with simple, descriptive fields, drop-..., and can be saved at any point throughout the process, so you can work through it at your own pace. To... -
Grad School Advice Part 2: Building Community
TypeBlog PostPublishedOct. 6, 2016, 2:30 p.m....Improvements that can be made to the grad school process 22:41 - END : Niroshi and Ben's favorite advice...Future of Research Looks to Improve the Research Process Browse our Career Posts Resources on Addgene.... -
Why Do I Need an MTA Anyway?
TypeBlog PostPublishedMay 1, 2014, 8:23 p.m....scientific sharing. So we set out to streamline this process. We take pride in facilitating the exchange of ... and by whom. For Recipients: Streamlined Processing: When you place an order, Addgene generates the... -
Let There Be LITE Plasmids
TypeBlog PostPublishedNov. 21, 2013, 3:56 p.m....of optogenetic control of endogenous cellular processes." The LITE system can now be applied to unraveling...genetic and epigenetic players in normal biological processes and disease states. While LITE plasmids have clear... -
SciComm with the Experts at Science in the News Part 2
TypeBlog PostPublishedApril 27, 2017, 2:30 p.m....The SITN Editorial Process -Vini discusses the importance of the editorial process and peer-to-peer feedback... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m.... be expensive and time-consuming to repeat the process with these new photoconvertible proteins. With ...state after excitation at 561 nm. This imaging process worked well with the common GFP variants EGFP, ... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m....Homologous recombination is the process by which nearly all domains of life repair genomic damage, specifically...Researchers have long taken advantage of this natural process to integrate protein tags into the genomes of S... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog PostPublishedJune 8, 2023, 1:15 p.m....Knowledge-Base, seeking to create an iterative process that is constantly becoming better, with more data...spatial analysis of cells in complex tissues. Proceedings of the National Academy of Sciences, 117(52),... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m.... original publication. To help facilitate this process, we explain how to cite plasmids from Addgene right...much information as possible during the deposit process. Special collections pages In an effort to make... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....existing relationships as part of the job application process can dramatically increase the chances of being ...document. 6) Use your network! During the entire process, use whatever means you can to get your resume ... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog PostPublishedJune 10, 2025, 1:15 p.m....downstream from the Cas target. Conveniently, the process doesn’t cause double-stranded breaks and can deliver... CRISPR-Cas systems by Tn7-like transposons. Proceedings of the National Academy of Sciences of the United... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m....mark it as a target for other immune cells, in a process known as opsonization. In antibody-dependent complement... the pathogen the antibodies are bound to in a process known as phagocytosis. Other functions We’re still... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog PostPublishedNov. 29, 2016, 3:30 p.m....one step. Challenges in the LEGO science video process One major challenge when we started was creating...laboratory. When we started, the hardest part of the process was to embed personality in pieces of plastic, ...