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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 43 results for: PROC

Showing: 1 - 20 of 43 results

  1. Plasmids for Stem Cell Research

    Type
    Collection
    ...with transgene-free induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83...somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. ... temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek Fibroblasts...conversion of human fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8...self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32....

  2. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...improved genetically encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9...and Golgi Zn2+ with genetically encoded sensors. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. ... cells revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann...high-frequency release at Schaffer collateral synapses. Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115...in muscles and neurons of Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2019 Aug 1. Alexander Gottschalk..., the activity of specific genes and cellular processes, or other factors like environmental conditions...largely preserving spatial and temporal cellular processes. Note that many of the articles linked below include...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...mammalian cells by tetracycline-responsive promoters . Proc Natl Acad Sci U S A, 89 (12), 5547–5551. https:/...mutations yield expanded range and sensitivity . Proc Natl Acad Sci U S A, 97 (14), 7963–7968. https:/...

  4. Neurodegeneration Research Collection

    Type
    Collection
    ...understand its function and pathology. Mohanty et al. Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral...disease and implicate a wide range of cellular processes. The vast majority of cases (90-95%) of ALS are...al. Cell Rep Methods. 2022 Mar 28. Follow APP processing and dynamics using APP and Bace1 plasmids . Capitini...

  5. The Pleiades Promoter Project

    Type
    Collection
    ...MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci U S A. 2010 Sep 21;107(38):16589-94...

  6. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...CRISPR labeling of chromosomal loci in human cells. Proc Natl Acad Sci U S A . 112(10):3002-7. PMID: 25713381...arrays. Separate Cas proteins then express and process the CRISPR loci to generate CRISPR RNAs (crRNAs...-CRISPR RNA (pre-crRNA). (2) The pre-crRNA is processed into individual crRNAs by a special trans-activating...non-homologous end joining (NHEJ) , an error-prone process that produces insertions/deletions disrupting a...a DSB at the target genomic locus. Repair will proceed through NHEJ or HDR depending on the experimental...

  7. Antibody Guide

    Type
    Collection
    ...randomized reorganization process, creating a unique binding site. This process, known as V(D)J recombination...delaying a process through sequential rounds of validation. Validation of an antibody is a process separate...binding partners, location in the cell, and sample processing can hide or reveal epitopes on an antigen. Monoclonal...can interfere with the conjugate or conjugation process. Sodium azide, an antimicrobial agent, can block...proteins to detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody...available. Tissue samples may need additional processing steps, such as sonication. Denature proteins,...proteins from a complex mix. Overview of application Process the tissue or cell culture samples to extract proteins...

  8. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...guaranteed titer, and will give you the option to proceed with your order, or not, without a guaranteed titer...that email within 10 business days in order to proceed with any adjustments to the original order. Turnaround... by your institution. Our final step of the QC process, viral genome sequence verification, may not yet...days of ordering. We will give you the option to proceed with your order, or not, with a longer turnaround...this email within 10 business days in order to proceed with any adjustments to the original order. Experimental...thorough shipping documents and following import procedure, it is the responsibility of the importer to pay...

  9. AAV Packaged on Request

    Type
    Collection
    ...purification Addgene’s comprehensive quality control process: Titering Purity assessment Sterility testing Viral...plasmid on its material page. Request and Ordering Process AAV production will take approximately 6–8 weeks...information must be added before we can begin processing your order. MTA Approval 1+ business days, varies...from the providing scientist through an MTA. This process typically takes 1–4 days, depending on your institution...prep, and perform comprehensive quality control processes to make sure it is ready for your experiments....

  10. mTOR Pathway

    Type
    Collection
    ...fuel these energy-intensive processes. mTORC1 also blocks catabolic processes including autophagy and lysosome...regulates the switch from anabolic to catabolic processes. Active mTORC1 promotes protein and lipid synthesis...

  11. Distribution to Industry

    Type
    Collection
    ...more about Addgene's MTA process please visit our Help Center . The MTA Process: Once your order has been...approve the MTA documents, your order will be processed and shipped. Industry Distribution Policy A portion...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce size and...Editing in Monocot and Dicot Plants Plant PCR-based procedure to rapidly generate multiple sgRNA expression ...Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple functional gRNAs...

  13. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...15574 GAL 25Q+ProCFPp303 HTT CFP GAL1 Huntington's Susan Lindquist 15575 GAL 103Q+ProCFPp303 HTT CFP GAL1...15578 GAL HA25QDProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist 15579 GAL HA103QDProCFPp303 HTT HA... Huntington's Susan Lindquist 15570 GAL HA25Q+ProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist...GAL1 Huntington's Susan Lindquist 15584 GAL FLAG25QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's Susan Lindquist...Lindquist 15585 GAL FLAG46QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's Susan Lindquist 15586 GAL FLAG72QDProCFPp303...GAL1 Huntington's Susan Lindquist 15589 MET FLAG46QDProCFPp303 HTT FLAG, CFP MET1 Huntington's Susan Lindquist...Lindquist 15590 MET FLAG72QDProCFPp303 HTT FLAG, CFP MET1 Huntington's Susan Lindquist 15591 MET FLAG103QDProCFPp303...

  14. Plan Your Experiment

    Type
    Collection
    ...genetic manipulation to model a specific disease or process of interest. Do you want to: Generate complete ... method of delivery and the cell type. Before proceeding, we recommend asking labmates/colleagues, searching... genes modulate transcription and nascent RNA processing. Nature Structural & Molecular Biology , 30 (...

  15. Antibodies

    Type
    Collection
    ... our production process? At Addgene, we provide transparency in our production process by...

  16. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Addgene collection? Click here to start the deposit process and have your plasmids added to the list. You can...more information and assistance with the deposit process. Want more information on the wide variety of Cas...

  17. Mammalian RNAi Tools

    Type
    Collection
    ...mechanism. Since it is pre-processed, it can bypass the Dicer cleavage step and proceed to binding of RISC. ...

  18. Promega Plasmid Collection

    Type
    Collection
    ...product pages. Orders of the 20 µg DNA size are processed and shipped within 6–8 business days. For more...more information about the ordering process, check out our help center . Plasmid Collection Browse, sort...

  19. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...also kindly provided this protocol detailing the process for packaging these transfer vectors and generating...pAdx-CMV-LacZ is uniquely suited to simplify the cloning process as it can be used in blue-white screening and forms...

  20. Deisseroth INTRSECT Collection

    Type
    Collection
    ... present, the introns are excised during mRNA processing, producing a functional molecular tool (C,F)....Deisseroth Lab maintains a Standard Operating Procedure (Link opens in a new window) with general principles...
Showing: 1 - 20 of 43 results