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Showing: 1 - 20 of 523 results

  1. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...quality control (QC) processes to screen for and reduce these errors. These processes allow us to have a...developed a process for identifying, categorizing, and resolving a variety of QC issues. This process ensures...discrepancies. While Addgene’s QC process adds time to the deposit process, we believe this to be non-negotiable...research, these errors can affect experimental processes, results, and subsequent conclusions. Being vigilant... edit, and easy to share. However, the cloning process — including the propagation of plasmids in bacteria... [email protected] to get started! Addgene’s QC process Addgene prioritizes the sequence quality of the...provided, each deposit goes through the same QC process to ensure all plasmids pass our high-quality standards...

  3. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...We’ve talked a lot about the quality control process at Addgene by introducing our new sequencing partner...regularly use Snapgene for our quality control process because of its expansive feature library and useful...Identifying plasmid features While our quality control process for incoming plasmids can vary based on the contents...

  4. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ... issues.  The permanent residence “green card” process is a necessary and often overlooked part of career...start planning for your visa and or green card process well before addressing your career alternatives... benefit from the applicant thinking about the process early, keeping good records, and planning for the...organizations do not have the April 1 H-1B queue process or quota like industry, these organizations can...achievements. Participating in the scientific review process as a reviewer as proof of your judging the work...You can still assist your PI in the peer review process, but remember you cannot use it as documentary ...that you can take to prepare for the green card process Update your CV and bio sketch whenever you have...

  5. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...genomes. Nern A, Pfeiffer BD, Svoboda K, Rubin GM. Proc Natl Acad Sci USA. 2011 Aug 23;108(34):14198-203...Allak B, van Drunen E, Kanaar R, Berns A, Jonkers J. Proc Natl Acad Sci USA. 2001 Jul 31; 98(16): 9209–9214...

  6. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...enhance magnetic cancer cell capture. Fierer et al (Proc Natl Acad Sci U S A. 2014 Mar 17. PubMed) Peptide...through engineering a bacterial adhesin. Zakeri et al (Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7....protein-peptide interactions. In a study published in Proceedings of the National Academy of Sciences in March,...SpyLigase system for the first time? Howarth: The Proceedings of the National Academy of Sciences paper [published...

  7. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... gene expression: Implications for gene therapy. Proc Natl Acad Sci U S A, 95(19), 11377. https://doi....Undergoing Spontaneous Degeneration in Tissue Culture. Proc Soc Exp Biol Med. 84(3), 570–573. https://doi.org...encode proteins essential to initiating the whole process – such as the viral DNA polymerase – it makes sense...

  8. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ... “Ligand-Regulated Site-Specific Recombination.” Proc. Natl. Acad. Sci. USA 92(June): 5940–44. PubMed ...Using a Ligand-Dependent Chimeric Cre Recombinase.” Proc Natl Acad Sci U S A 92(15): 6991–95. PubMed PMID...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5547-51.... hybrid derived from the trp and lac promoters.” Proc Natl Acad Sci U S A. 80(1) (1983): 21-5. PubMed ...over the TetO elements, allowing transcription to proceed (pJKR-H-tetR from the Church lab uses this system...Response Element (TRE)), allowing transcription to proceed. When tetracycline or one of its derivatives is...linked in groups of 6), allowing transcription to proceed. When cumate is added, it binds cTA, resulting ...

  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...piggyBac transposase tools for genome engineering.,” Proc. Natl. Acad. Sci. U. S. A., vol. 110, no. 25, pp...provides a simple tool for conditional cell ablation.,” Proc. Natl. Acad. Sci. U. S. A., vol. 105, no. 38, pp...to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, as it relies on...incorporated, and used during the differentiation process. Moreover, a splinkerette-PCR assay may be used...

  11. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7....

  12. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...topoisomerase I in Escherichia coli is sequence specific."  Proc Natl Acad Sci U S A.  1991 Nov 15;88(22):10104-8...restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique relies on the...insert, the magic of TOPO cloning happens. Basic procedure Let’s break down the steps needed for TOPO cloning... everything you need for the next step before proceeding. Pre-warming your antibiotic-containing plate...

  13. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... for real-time multicolor luminescence imaging.” Proc Natl Acad Sci U S A 112(14) (2015): 4352-6. PMID...activation, you can’t use fluorescence to monitor processes directly affected by light. Long-term light exposure...

  14. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...genomics of biotechnologically important yeasts. Proc. Natl. Acad. Sci., 113, 9882-9887. DOI: 10.1073/...

  15. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34): p. 14234-9. ... temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012. 109(27): p. E1848-57....cell type, bypassing the lengthy processes of reprogramming. The process was first observed in the regenerating...during the reprogramming process as they are propagated in vitro as part of the process. This opens up a wide...damaged cells. Here, I will discuss the two major processes by which an adult somatic cell is converted to...regeneration and repair and situations where one process is favored over the other. Cell conversion happens...different cell type. Transdifferentiation In this process, differentiated adult somatic cells are converted...

  16. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...immunohistochemical reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115...would become busy and potentially detract from the process being depicted, we can’t forget that those other...plasmids through Addgene, don’t hesitate to start the process! Addgene depositors who study chromatin The PIs...

  18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...with the endogenous tRNA-processing system. Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...in rice plants. Yang was intrigued by the tRNA processing machinery present in almost every living organism...gRNAs with any 5’ leading nucleotide could be processed, a distinct advantage over other methods that ...is its universality; robust and efficient tRNA processing is common to prokaryotes and eukaryotes. Though...

  19. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...CRISPR labeling of chromosomal loci in human cells.” Proc. Natl. Acad. Sci. USA 112(10) (2015): 3002-7. PubMed...

  20. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...protein expressed by recombinant vaccinia virus. Proc Natl Acad Sci U S A. 1991;88(20):8972-6. Moks T,...
Showing: 1 - 20 of 523 results