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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 577 results for: SIM

Showing: 181 - 200 of 577 results

  1. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...postdocs. Unfortunately, even when universities have simple postdoc numbers sorted out there can be gaps in...important take-away from this session was the idea that simply satisfying a percent requirement for diversity ...difficult question but not one that should be abandoned simply because it is difficult and is key to the continued...

  2. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP...-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers H. Cell. 2010 Oct 1;143(1):134-44. ...Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods. 2011 Mar;8(3):253-9. doi: 10.1038...

  3. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...cells? Immunoblotting or simply the western blot, or western, is one of the simplest methods to detect the...could contain unintended cross-reactive proteins. Similarly, if you vary the experimental conditions from ...

  4. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...the go-to choice for studying mammalian biology. Similar to any model, mice are not without their problems...manipulate the mouse genome using CRISPR/Cas9. Similar to the human genome, the mouse genome is made up... finally, we have a technology, CRISPR, that is simple in concept, straightforward to use, and robust ...

  5. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...I changed jobs. I highly recommend taking on a similar position in your field of interest before you dive... solution attempted was more meetings, but this simply seemed to create more bureaucracy. As with any ...anticipated and there can be a glut of individuals with similar skill sets. This is not to say that all commercial...

  6. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ... cell from the clinic. The 2nd generation is similar to the first one with the exception that the HIV...must be chosen carefully. Often researchers want a simple set-up where the gene of interest is expressed ...experience in transfection, CaCl2 transfection is a simple and cheap technique that should work well. Quickly...

  7. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...pitfalls of accepting alternative facts. Because, similar to scientists, the legacy of each government and..., challenged us. I worried the march would be a simple, unquestioning celebration of science, at a time...research scientist would be a career that could simply cease to exist. But then November hit and panic...

  8. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...lysing cells and removing cellular debris look very similar. However, keep in mind that kit components (i.e... Figure 2: Plasmid isolation workflows are similar across kits. Lyse your bacteria to release the ...can be more flexible in this situation as you can simply add more beads to your sample. But, you’ll need...

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...likely to express the target at a robust level, similar to that in samples of interest. If you are working...   If your protein of interest is expressed at similar levels in your cell lines, you can consider a cell...transiently overexpressed in CHO cells and displayed similar labeling patterns on different primary blood leukocytes...

  10. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...audience blindly receiving them; it results in highly similar content and the absence of iterative design; it...published 1 Jan 2013, no longer available), is a simple catalogue of reported sgRNA designs. The second...The third, WGE (published 15 Sep 2015), is more similar to EENdb than CrisprGE, but is also a good example...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...protein that enables imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin ...mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring the activities of CaMKII and RhoA...PhoCl between an enzyme and an inhibitor domain similarly allows for control of enzymatic activity. In addition...

  12. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ... of the best ways to learn about a new field is simply to know where to look and how to start. There is...  About the author Niko McCarty is the editor of Asimov Press and is designing a genetic engineering curriculum...

  13. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...structures demonstrating “the structure is strikingly similar to the design model at atomic resolution” (Kuhlman... design is possible. Although Top7 was designed simply to be stable and novel, the principles that created...

  14. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...in the biological sciences and techniques that simplify and expedite research. We recognize, however, ... blog! To sign up to write for the Addgene blog simply email us. We can’t guarantee that all those who...

  15. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ...that within that article you’ll find new plasmids. Simply click on the badge to be taken directly to the ...an account on PubMed. Once you have an account, simply click on your account name in the top right corner...

  16. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...application. The submission process is easy, with simple, descriptive fields, drop-down boxes when appropriate...can work through it at your own pace. To submit, simply click "Contribute Data."    Figure 6: Contributing...

  17. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...ladders are not next to empty wells. Most people will simply load an extra ladder or technical replicate (ie... leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget to start it...blocking time! Fill the time with smaller tasks, or simply enjoy some well-deserved down time while waiting...

  18. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support Specialists... Placing an order on Addgene’s website is quite simple, even if you’re using a PO number. Follow these...

  20. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...DePace’s yearly planning meetings revolve around two simple worksheets, each of which is filled out separately...Extracurricular Activities for a Strong Science Career Path 7 Simple Strategies to Resolve Conflict With Difficult Supervisors...
Showing: 181 - 200 of 577 results