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We narrowed to 359 results for: Sele

Showing: 181 - 200 of 359 results
  1. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...reasons. Exposure to antibacterial drugs provides a selection pressure, killing off bacteria that are susceptible...stop a treatment early, you may be un-wittingly selecting for less susceptible bacteria that will be more...
  2. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...variables within an experiment, both the design and selection of the positive control plasmids should be highly... Expression Level of Gene X   The design and selection of proper experimental controls is not a trivial...
  3. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...chloramphenicol resistance cassette for positive selection and a p15A origin of replication, making it incompatible...Your strain may require additional antibiotic selection or a special growth media, or if your plasmid ...
  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...necessary to set up a sequence of gates to be able to select and precisely measure your cells of interest. In...                                                                                                        B.      Figure 5: (A) A gate to select the negative live population from my live-dead ...
  5. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...Thermo Fisher Scientific's Biotinylation Reagent Selection Tool. AviTag and BirA Proteins can also be biotinylated...Photobiotinylation Photobiotin can be used to non-selectively label DNA and RNA. Photobiotin acetate consists...
  6. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...intended edit is the biggest determinant when selecting a type of donor. ssDNA templates (referred to ...large insertions such as fluorescent proteins or selection cassettes. This class of template should have ...
  7. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...a unique and powerful high-throughput screen to select variants based on performance metrics specifically... was identified from a high-throughput screen, selected for increased voltage response, brightness, and...
  8. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ... into the final translated products. Negative selection markers built into all plasmids in the kit help...studies monitoring sequence dynamics under various selective pressures: Miller, Corwin, et al. "Adaptation...
  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...untagged or add a versatile N- or C-terminal HA tag. Selectable and stable: A puromycin resistance gene makes... infected with 96 h lentiviral supernatant and selected with puromycin. Puromycin-resistant cells were...
  10. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...mouse embryonic stem cell (ESC) line, enrich and select for cells that had successfully incorporated the...eggs, circumventing the need for enrichment and selection offered by mouse ESCs. For example, we often screen...
  11. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...effective and accurate way possible it’s important to select the right test for the job (and, uh, gentle reminder...run it.) You don’t need to be a mathematician to select the right statistical test, just do your homework...
  12. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...skills who fulfill a long-term function. When a select group of people are responsible for a group of ...of criticism or judgment - nobody likes to feel useless, and excess people who are not contributing will...
  13. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...experiments, care still needs to be taken when selecting which particular AAVs to use for your research...’s value greatly. Applying this mantra to AAV selection, there are three important factors related to ...
  14. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...marker (Fluorescent protein or selection marker) allowing for selection of the transduced cells. In this...
  15. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...identify which insertions were positive or negative selected for during the experiment (Sandoval-Villegas et...and host range are important to consider when selecting a transposon for a mutagenesis screen. For example...
  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...that there is no universal scoring system for selecting a gRNA, as the method of producing the guide (...phenotype is due to an on-target effect. Conclusions Selection of gRNAs for an experiment needs to balance maximizing...
  17. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...broken down broadly into three main steps: 1) Selection and expression of your opsin of choice in the ...features of each of these steps. Today we’ll discuss selection and expression of your opsin, but keep you eyes...
  18. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have..., with a choice of either hygromycin or Basta selection. Yang Lab Single Transcript Multiplexing Plasmids...
Showing: 181 - 200 of 359 results