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We narrowed to 213 results for: ada.2

Showing: 181 - 200 of 213 results
  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...cellular responses in addition to antibody response 2) very high immunogenicity 3) and long-lasting immune...which target complementary mRNA sequence for degradation. Viral introduction of shRNA plasmids can induce...
  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication ...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...
  3. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...PMID: 20543841. PubMed Central PMCID: PMC2920597. 2. Nathans, Daniel. "Puromycin inhibition of protein...the combined effect of protein synthesis and degradation. These processes control the abundance of proteins...
  4. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ... scientific communication ecosystem Figure 2: The scientific ecosystem, enriched by open access...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...
  5. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ... would release them into the cytosol. Fig. 2: Endogenous sources of duplexed RNA DNA:RNA hybrids...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...
  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ..., N. and Hamilton, D. 1981. PubMed PMID: 6276558. 2. Site-specific DNA recombination in mammalian cells...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...
  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...types within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should...the antibody in the same tube.     Figure 2: Schematic showing the use of an orthogonal approach...monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., 2024.   Once an antibody...
  8. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...strain (which will be covered in more detail in part 2 of this blog series) controls the cell types in which...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...
  9. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ... more from the authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression... of various G-protein-coupled receptors. Image adapted from Ino et al. 2022, under CC-BY license.   Find...activation by shift in cellular localization. Image adapted from Farahani et al. 2022, under CC-BY license....responses are highly resistant to pH changes. Image adapted from Koveal et al. 2022, under CC-BY license.   ...
  10. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ...15.4%) 46 (59%) of these students were in the last 2 years of their degree. Students filled in a pre-program...MasScience, ECUSA, IMFAHE and Bostonadas. Follow her on twitter @Bostonadas.         Rocío López-Diego MD...mindedness, and the ability to accept feedback and adapt their mentoring styles to the mentee’s personality...
  11. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...positive cells (Martin et al., 2019). Figure 2: Editing efficiencies using base editing reporters...reporter, and neomycin resistance reporter. Image adapted from Katti et al., 2020. As a proof of concept...Gaudelli et al., 2017). GO is thus a flexible and adaptable tool to identify and enrich for base-editing events...
  12. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...to the break room for a sip of water.  Fig 2: Blugene likes to spark creativity in out on the open...your problem. In a study outlined in Originals by Adam Grant, a creativity expert at Stanford conducted... with the group (because they will fight more adamantly for their solution), selecting a devil’s advocate...
  13. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...more tips on using social media as a scientist here. 2) Public engagement - Participate in science festivals...to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your own citizen...
  14. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...overview of these new variants:   Figure 2: Inner mitochondrial membranes imaged with structured...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...
  15. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...PMID: 27151215. PubMed Central PMCID: PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery...are quickly cleared from the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR...
  16. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...of these issues are not particularly new. Chapter 2, “The Professional Scientist,” addresses the challenges...difficulties in dealing with climate change, the degradation of the U.S. environment, and a decline in the...
  17. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next time is ...formulated the presentation as follows (which could be adapted for any topic--not just CRISPR): Who am I? Where...
  18. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...interesting things to do, feel free to crowdsource! 2. Free food generally gets people to show up If you...fashion. 9. Ask for help My grandma used the old adage “many hands make light work” all the time. She was...
Showing: 181 - 200 of 213 results