We narrowed to 213 results for: ada.2

...acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes...mitochondrial (mito-mCherry-1xFLAG) localization. Adapted from Liu et al. 2021. Anti-FLAG and anti-HA...

...3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication ...designed for plant expression, PTGs will likely be adaptable to many different biological systems. Although...

... would release them into the cytosol. Fig. 2: Endogenous sources of duplexed RNA DNA:RNA hybrids...Transcription, translation, and prone to degradation – those are the words that describe RNA! Double ...

... scientific communication ecosystem Figure 2: The scientific ecosystem, enriched by open access...asapbio.org/preprints-and-covid-19 Ettinger CL, Sadanandappa MK, Görgülü K, Coghlan KL, Hallenbeck KK, Puebla...

...PMID: 20543841. PubMed Central PMCID: PMC2920597. 2. Nathans, Daniel. "Puromycin inhibition of protein...the combined effect of protein synthesis and degradation. These processes control the abundance of proteins...

..., N. and Hamilton, D. 1981. PubMed PMID: 6276558. 2. Site-specific DNA recombination in mammalian cells...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...

...types within a sample to expected labeling (Figure 2). The panel of antibodies used in this approach should...the antibody in the same tube.     Figure 2: Schematic showing the use of an orthogonal approach...monoclonal antibody, ** = recombinant antibody. Figure adapted from Biddle et al., 2024.   Once an antibody...

...main decisions to make: 1. Which modification? and 2. Which viral vector should be used? Modifying gene...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

...strain (which will be covered in more detail in part 2 of this blog series) controls the cell types in which...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...

...details below) can also be adapted to a competitive format. Figure 2: Overview of using Competitive...

... more from the authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression... of various G-protein-coupled receptors. Image adapted from Ino et al. 2022, under CC-BY license.   Find...activation by shift in cellular localization. Image adapted from Farahani et al. 2022, under CC-BY license....responses are highly resistant to pH changes. Image adapted from Koveal et al. 2022, under CC-BY license.   ...

...15.4%) 46 (59%) of these students were in the last 2 years of their degree. Students filled in a pre-program...MasScience, ECUSA, IMFAHE and Bostonadas. Follow her on twitter @Bostonadas.         Rocío López-Diego MD...mindedness, and the ability to accept feedback and adapt their mentoring styles to the mentee’s personality...

...positive cells (Martin et al., 2019). Figure 2: Editing efficiencies using base editing reporters...reporter, and neomycin resistance reporter. Image adapted from Katti et al., 2020. As a proof of concept...Gaudelli et al., 2017). GO is thus a flexible and adaptable tool to identify and enrich for base-editing events...

...to the break room for a sip of water.  Fig 2: Blugene likes to spark creativity in out on the open...your problem. In a study outlined in Originals by Adam Grant, a creativity expert at Stanford conducted... with the group (because they will fight more adamantly for their solution), selecting a devil’s advocate...

...more tips on using social media as a scientist here. 2) Public engagement - Participate in science festivals...to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your own citizen...

...overview of these new variants:   Figure 2: Inner mitochondrial membranes imaged with structured...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

...PMID: 27151215. PubMed Central PMCID: PMC4938170. 2. Zuris, John A., et al. "Cationic lipid-mediated delivery...are quickly cleared from the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR...

...of these issues are not particularly new. Chapter 2, “The Professional Scientist,” addresses the challenges...difficulties in dealing with climate change, the degradation of the U.S. environment, and a decline in the...

...would help them grasp some of the key concepts (Fig. 2). One thing I will likely change for next time is ...formulated the presentation as follows (which could be adapted for any topic--not just CRISPR): Who am I? Where...

...interesting things to do, feel free to crowdsource! 2. Free food generally gets people to show up If you...fashion. 9. Ask for help My grandma used the old adage “many hands make light work” all the time. She was...