... involved in developing tools and solutions to research problems. This movement calls itself the Open ...and is part of a bigger movement in a variety of research sectors to provide open source technologies and...and spaces where interested parties can do research. This interest manifested itself in the creation of...collaboratively to solve, we want to focus our research on worm locomotion, but our worms are constantly...automated microscope  to keep our worms and our research goals in focus. Developing these skills relies...submit our new strains back to the CGC for other researchers to use. Although we haven’t yet made our final...University of Victoria. After a decade at Microsoft Research working on speech recognition, he returned to ...

...by guest blogger Fredrik Wermeling, leader of a research group at the Centrum for Molecular Medicine (CMM... different gRNAs. It has been suggested that researchers should use ~400X more cells than gRNAs in a screen...are addressing a well-defined, hypothesis driven research question. Examples of custom CRISPR screens Differentially...analyses like RNAseq are getting lower and lower, the research community is generating a massive amount of descriptive...such data. These targeted screens can allow a researcher to test which of the differentially expressed...embedded. These were kindly donated by six different research constellations: Doench/Root (2), Zhang (3), Wu...screen! Final note I am very grateful to the researchers letting us use their reference libraries thereby...

...show you how Drosophila can be used to push your research in new and exciting directions. Drosophila are...the fourth and Y chromosomes are miniscule, so researchers generally do not manipulate them. Essentially...accessible resource out there to help keep your research moving. Below, you’ll find some great introductory... resources. As you dive deeper into Drosophila research, you’ll find more resources that may be more tailored...look up information about your fly gene using the search box in the upper right hand corner. They have video...Drosophila reagents are generously shared among the research community. They maintain many fly stocks that ...stocks are available at Bloomington, but you can search for various fly reagents on the Bloomington website...

...While much of CRISPR research has focused on genome editing, numerous discoveries have been made using...But dCas9 does not cleave the DNA. Much of the research using dCas9 has focused on transcriptional activation...antibody targeting the 3xFLAG tag, allowed these researchers to isolate dCas9 with the bound genomic locus...flavors to this methodology. Using this technique, researchers have begun to make important discoveries in various... region, other forms of enChIP, and additional research conducted in the Hodaka Fujii Laboratory, please...enChIP) using CRISPR." Biochemical and biophysical research communications 439.1 (2013): 132-136. PubMed PMID...the analysis of bacterial genome functions." BMC research notes 11.1 (2018): 387. PubMed PMID: 29898790....

...a little over half of our orders coming from researchers outside the USA, having some materials available...data and resources to increase accessibility to researchers around the globe. Addgene’s educational resources...become our collection pages. These pages allowed researchers to easily access and compare their options for...expression types, and more, all of which help a researcher understand if a material is right for their experiment...2023, we had over 900 posts, which were read by researchers all over the world — and we really do mean all...educational resources’ value is feedback from the researchers who use them. The best part of any conference...crowdsourcing experimental help. We love it when a researcher reaches out to ask if they can help us create...

...depending on the part of the company involved: Research & Development (R&D) teams at pharma companies ...companies carry out preclinical and clinical research to discover and test the best new therapeutics. Consultants...among physicians and scientists about clinical research, drugs, the diseases they can treat, and diagnostic...client might need to get a large amount of market research done to inform a go/no-go decision in a very short...PhD, an MD, or someone trained in any aspect of research in biology or medicine you will bring an essential...global health organizations, investment and equity research firms, biotech start-ups, venture capital firms...consultants. I have even seen consultants go back to research and bench science, with a newfound appreciation...

...to share curated information about AAV usage in research applications. Scientists around the world can ...Reports receive a DOI for citation, crediting researchers when their data is used by the community to drive...in materials sharing, information sharing, and research reproducibility while expanding our suite of materials... materials and services to support more research and innovation around the world. As always, we seek to...opening up science to help create new knowledge and research opportunities. I have spent the last 9 months ...same is Addgene’s commitment to supporting the research community through sharing science and distributing...

...over three decades. Widely used across various research applications, they have become indispensable tools...and organisms. They can be used for a variety of research purposes, like creating stable cell lines, delivering...vectors work with regards to integration, and how researchers have modified them to prevent this integration... DNA… forever!  This can be an advantage for researchers looking to generate long-lasting changes in cells...genome (Figure 1). IDLVs were first discovered by researchers investigating HIV integrase mutants in the early... choice for certain types of clinical or basic research applications. Considerations for IDLV use If you...you know how IDLVs could be useful in your own research, we wish you the best of luck with any experiments...

...Morgan Model organisms are vital tools used by researchers around the globe. These organisms share many ...most preferred mammalian model organism of many researchers: the mouse (Mus musculus). Mice have many advantages... human diseases and have successfully helped researchers develop a variety of therapies. For example, ...fly. The fruit fly has been used in scientific research for over a century but was brought to the forefront...Morgan, who is known as the “father” of Drosophila research. Morgan discovered that genes were found within...is a commonly used model organism in scientific research. Yes, the same kind we use in breads and other...molecular biology reference Find plasmids for your research ...

...within a cell,” “Does this disease change the architecture of my cells,” or “How does this mutation impact...about IF, direct and indirect. For direct IF, researchers use a single antibody to visualize their target...their results and corresponding protocols. Other researchers may also have tested an antibody in IF and will...organism that is underrepresented in the biomedical research community. You may have to take some risks on ...provide you not only answers to your most pressing research questions, but also stunning images (am I the ...results as artwork at home?). Take your time to research antibodies, protocols, and appropriate controls...Additional Resources on the Addgene blog Learn about research applications of antibodies in Introduction to ...

...latently infected cells. To truly cure HIV-1, researchers need to vanquish these hidden viral reservoirs...“shock and kill” the HIV-infected reservoir, researchers have previously used histone deacetylase (HDAC...could permit some viral protein production, so researchers are also interested in precisely excising the... were used for proof-of-concept studies, but researchers believe that using CRISPR/Cas9 could improve ...represent exciting advances in preclinical HIV-1 research that should be pursued in animal models, and it...benefit many others and accelerate the pace of research. We at Addgene hope to see the problem of in vivo...updated CRISPR Guide! Find CRISPR Plasmids for Your Research Browse gRNA Design Tools  ...

... discovery. Over 150,000 plasmids empowering researchers worldwide. Countless breakthroughs enabled by...characterized antibodies, and our contributions to key research papers are already inspiring higher standards ...as DNA or bacterial stabs — ‌and reinventing our search experience so you can find exactly what you need... evolving rapidly, and with it, the needs of researchers worldwide. Through our growing community on Bluesky...incredible journey. Together, we're not just sharing research materials — we're accelerating the future of science...critical to enhance reproducibility in biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife...

... Find Other Kits for Your Research Find Pooled Libraries for Your Research View our Hot Plasmid Pages... scalable manner, Wood said. Thankfully, the researchers didn’t have to discover new ways to activate ...library, obtained the appropriate cDNAs from other researchers, and cloned them into lentiviral vectors to generate...lentiviral expression vector and attached a DNA barcode to each to make the library usable for pooled screens... these available as widely as possible.” The researchers have plans to expand the library to include activators...

...expand the toolbox for researchers looking to study norepinephrine, the Patriarchi lab continues to work...sensors was developed, named GRABNE, which helped researchers visualize norepinephrine (Feng et al., 2019).... and were only available as green probes. The Patriarchi Lab aimed to overcome these limitations by developing...nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with this blog post. References...Benke, D., Weber, B., Bohacek, J., Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for...

...for jobs posted online as one part of their job search. This is especially true of scientists seeking ...Follow job boards and check them regularly Use your research skills to determine which job boards list positions...clearly describe that the roles do not involve bench research. I am always surprised at how many applicants ...diffrent types of positions (for example, bench research vs. project managment) your resume should also...post for additioanal advice for the science career search.  You might also enjoy the other posts in the Addgene... job search...

...collection of plasmids is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent...to either the N-or the C-terminus. Even if a researcher’s gene of interest isn’t in the ORF collection...in the Davidson lab for the last four years. “Researchers can make their own target if it is not already...can essentially cut and paste quite quickly. Researchers should keep in mind that a very simple sequential...deposit the tools he and his team created with the research community at large, "[Fluorescent proteins are...been very adamant that we distribute to as many researchers as we could, and Addgene is a wonderful vehicle...

..., I moved to Albion six years ago to work as a research associate after having earned a PhD in Developmental...the University of Paris VII. During my years in research, I developed several new plasmids, including: ... believe that a lot of European students and researchers are craving, like I was, for a better way to ...Biology. She has worked in a range of labs - cancer research, microbiology, virology, and genetics - before...scientists together from all over the world to exchange research materials in an easy way. She has a passion for...she can help the scientists in Europe with their research.   Europe, we are all geared up to answer your...

...Choosing a Research Project Finding and Joining Your Dream Lab Advice for Choosing a Research Project Advice... to get advice on choosing a lab, picking your research topic, starting a lab, and more. Before we start...others out, they'll help you too; both with your research and with getting published.” Connie Cepko 1....interested in leaving academia should present their research at conferences to obtain a “crash-course in all...tuned for the follow up blogs on "Choosing Your Research Project," “Advice for Starting Your Lab,” “Fun...

...biomedical research". The family entered the biomedical field’s spotlight after researchers discovered...animals have impacted humans through biomedical research. The Camelid family is composed of camels, llamas... llamas and alpacas (Figure 1). At the time, researchers were already on a quest for smaller forms of ...experiments, due to their simple structure    sdAbs in research These advantages have contributed to sdAbs becoming...variable domains (aka nanomice), which gives researchers the “best of both worlds” - a familiar lab model...

...Plasmids from a variety of research labs and hopes that these will help researchers build their knowledge ...efficiently in humans. This is why we cannot stop searching for novel solutions to treat rare diseases like...causes, and potential cures. We encourage any researchers working on ACVR1 or any of the pathways that ...about Addgene's Previous Work with Rare Disease Researchers Check Out Potentiall HIV Treatments with CRIPSR... Find ACVR1 Plasmids Find Plasmids for Disease Research on OurSpecial Collections Page ...