We narrowed to 284 results for: cki

1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...

2. CRISPR 101: Non-Homologous End Joining

   Type

   Blog Post

   Published

   April 16, 2015, 3:45 p.m.

   ...Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing...

3. Dos & Don'ts When Publishing a Scientific Manuscript

   Type

   Blog Post

   Published

   May 22, 2014, 4:02 p.m.

   ...journals as possible at the same time. Take your time picking the appropriate journal and explain in your cover...

4. Kiran Musunuru on the Newest TALEN Genome-Editing System

   Type

   Blog Post

   Published

   Dec. 10, 2013, 3:13 p.m.

   ... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...

5. Addgene: The First Twenty Years

   Type

   Blog Post

   Published

   Dec. 19, 2024, 2:15 p.m.

   ...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...

6. Antibodies 101: Fab Fragments

   Type

   Blog Post

   Published

   Sept. 5, 2024, 1:15 p.m.

   ...fragment. But this bivalency may cause problems for blocking purposes, since the fragment could bind not only...

7. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ... experiment! As a starting point, we recommend checking the literature and our AAV data hub to see if ...

8. The time and cost required to make a plasmid

   Type

   Blog Post

   Published

   March 23, 2023, 1:15 p.m.

   ... the traditional PCR/digest/ligate methods and picking the right one can save you time if you’re working...

9. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   Published

   Aug. 5, 2021, 12:15 p.m.

   ...surface display vectors to improve expression and trafficking of these biosensors.   Figure 1: Schematic...

10. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...between them, could affect the properties or trafficking of the fusion protein (Kimple et al., 2013). ...

11. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ... interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also produces green fluorescence...

12. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...in some applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...

13. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...and racks meant we had fewer errors in our LIMS tracking system. Though you might not have to communicate...

14. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...and systematically mutated it. Using a robotic picking approach to select cells with variant characteristics...

15. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However...

16. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ... postdocs were at institutions focused only on tracking hours, and 22% of postdocs were at institutions...

17. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA and...

18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ... our biosensor repository as we find ourselves lacking several crucial biosensors that would allow us ...

19. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    Published

    April 14, 2022, 1:15 p.m.

    ...nanobodies, are derived from a camelid antibody lacking a light chain, called a heavy chain antibody (Muyldermans...

20. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...their own work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and...