We narrowed to 245 results for: construct

1. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...guidance on how to make your own gene-tagging constructs below. Read on to find out how these plasmids...

2. A Look at Addgene's QC Process

   Type

   Blog Post

   Published

   May 6, 2025, 1:15 p.m.

   ...catalog containing over 150,000 sequence-verified constructs to share with scientists across the globe. This...

3. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ... for cells stably and highly expressing these constructs can be achieved by fluorescence-activated cell...

4. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...detailed information on cloning procedures used to construct the deposited plasmids. Molecules: We synthesized...

5. Plasmids 101: Protein Expression

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   Blog Post

   Published

   June 7, 2018, 1:17 p.m.

   ...modifications for proper protein function. Most DNA constructs designed for mammalian expression utilize viral...

6. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   Blog Post

   Published

   May 24, 2018, 1:20 p.m.

   ...circular plasmid via PCR. Typically, to make these constructs, developmental biologists have to extract RNA...

7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ...at the plasma membrane in living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate...

8. Corralling and Cornering the Goldilocks Antibody

   Type

   Blog Post

   Published

   Aug. 9, 2022, 1:15 p.m.

   ...sequencing to turn mAb clones into expression constructs to be conveniently modified and engineered.   ...

9. CRISPR in the Clinic

   Type

   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ... CRISPR therapy. They engineered a CRISPR-Cas construct targeting the diseased allele specifically. Early-stage...

10. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...terminator may be desired for multi-cistronic constructs where high termination efficiency is necessary...

11. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...Central PMCID: PMC3138296. 2. Sparta DR, et al. Construction of implantable optical fibers for long-term ...

12. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...sites (Geertsma, 2013). Once the phagemids are constructed, they are used to produce a phage library consisting...

13. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...FRET Find protocols and tips for making plasmid constructs Resources on Addgene.org Find fluorescent proteins...

14. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...vectors and DNA modules for assembly of CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized...

15. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...CRISPR/Cas9 induced break. Learn more about these constructs and the cell lines they’ve been used to create...

16. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...and invite you to make any fluorescent protein constructs you create in your own work available to the ...

17. The Scientific Conference Poster Session: Tips for Success

    Type

    Blog Post

    Published

    Aug. 29, 2019, 12:50 p.m.

    ... they can digest what you’re saying, provide constructive criticism, and ask thought-provoking questions...

18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ... a proof of principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...PGK promoter) and U6 promoter-driven guide RNA constructs, pAdSh.U6.gRNAS1 and pAdSh.U6.gRNAGFP, and also...

20. Addgene's Cancer Collection Pages Connect the Research Community

    Type

    Blog Post

    Published

    Sept. 6, 2016, 2:30 p.m.

    ...Its major appeal is that it enables the simple construction of vectors to study the function of any of these...